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Rat Recombinant Monoclonal SEZ6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 3 publications.

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Images

Western blot - Anti-Sez6 antibody [14E5] (AB252863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (AB252863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (AB252863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (AB252863), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (AB252863), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Not recommended
Not recommended
Mouse
Tested
Tested
Rat
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity.

Alternative names

Kiaa4158, Sez6, Seizure protein 6, SEZ-6, Brain-specific receptor-like protein C, BSRP-C

Recommended products

Rat Recombinant Monoclonal SEZ6 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 3 publications.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
14E5
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sez6 also known as seizure-related gene 6 protein plays an important role in the nervous system. It has a mass of about 110 kDa and is mostly expressed in the brain. Sez6 is part of a family of proteins that includes Sez6L and Sez6L2. This protein contains multiple complement control protein (CCP) domains which are important for its function in modulating neural activities.

Biological function summary

Sez6 modulates synaptic transmission by regulating AMPA receptor trafficking at the synapse. This protein participates in the complex of proteins involved in synaptic maintenance and plasticity. Through its effect on synaptic receptors Sez6 influences calcium influx and neurotransmitter release important processes for proper neural signaling and cognitive functions.

Pathways

Sez6 influences synaptic signaling and neuronal plasticity pathways. It particularly affects the glutamatergic signaling pathway altering the function of AMPA receptors. Sez6 interacts with other synaptic proteins like PSD-95 and glutamate receptors which mediate communication between neurons and adjust synaptic strength.

Associated diseases and disorders

Sez6 has been linked to neurodevelopmental disorders such as autism spectrum disorder. It is also associated with epilepsy as disruptions in synaptic regulation could lead to seizures. The connection to these disorders often involves altered interactions with proteins like NRXN and NLGN which are vital for synaptic formation and maintenance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Sez6 antibody [14E5] (ab252863), expandable thumbnail

    Western blot - Anti-Sez6 antibody [14E5] (ab252863)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed was in consistent with the literature (PMID: 27716410).

    Exposure time: 180 seconds.

    All lanes: Western blot - Anti-Sez6 antibody [14E5] (ab252863) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution

    Predicted band size: 107 kDa

    Observed band size: 170 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

    Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the rat hippocampus. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the rat cerebrum. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the mouse hippocampus. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] (ab252863)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Sez6 with ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the mouse cerebrum. The section was incubated with ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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