Rat Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--western-blot-img142443.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27822.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21055.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24579.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42154.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (AB255859)")
Publications
Filter by
- Molecular neurodegeneration 11:672016Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Pigoni et. al.PubMed 27716410
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Not recommended | Not recommended |
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity.
Alternative names
Kiaa4158, Sez6, Seizure protein 6, SEZ-6, Brain-specific receptor-like protein C, BSRP-C
Recommended products
Rat Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 14E5
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255859 is the carrier-free version of Anti-Sez6 antibody [14E5] ab252863.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sez6 also known as seizure-related gene 6 protein plays an important role in the nervous system. It has a mass of about 110 kDa and is mostly expressed in the brain. Sez6 is part of a family of proteins that includes Sez6L and Sez6L2. This protein contains multiple complement control protein (CCP) domains which are important for its function in modulating neural activities.
- Biological function summary
Sez6 modulates synaptic transmission by regulating AMPA receptor trafficking at the synapse. This protein participates in the complex of proteins involved in synaptic maintenance and plasticity. Through its effect on synaptic receptors Sez6 influences calcium influx and neurotransmitter release important processes for proper neural signaling and cognitive functions.
- Pathways
Sez6 influences synaptic signaling and neuronal plasticity pathways. It particularly affects the glutamatergic signaling pathway altering the function of AMPA receptors. Sez6 interacts with other synaptic proteins like PSD-95 and glutamate receptors which mediate communication between neurons and adjust synaptic strength.
- Associated diseases and disorders
Sez6 has been linked to neurodevelopmental disorders such as autism spectrum disorder. It is also associated with epilepsy as disruptions in synaptic regulation could lead to seizures. The connection to these disorders often involves altered interactions with proteins like NRXN and NLGN which are vital for synaptic formation and maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--western-blot-img142443.jpg "Western blot - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)")
Western blot - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed was in consistent with the literature (PMID: 27716410).
Exposure time: 180 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sez6 antibody [14E5] ab252863).
All lanes: Western blot - Anti-Sez6 antibody [14E5] (Anti-Sez6 antibody [14E5] ab252863) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rat IgG (H+L), HRP) (Goat Anti-Rat IgG H&L (HRP) ab205720) at 1/10000 dilution
Predicted band size: 107 kDa
Observed band size: 170 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img27822.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)
Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling Sez6 with Anti-Sez6 antibody [14E5] ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the rat hippocampus. The section was incubated with Anti-Sez6 antibody [14E5] ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sez6 antibody [14E5] ab252863).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21055.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Sez6 with Anti-Sez6 antibody [14E5] ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the rat cerebrum. The section was incubated with Anti-Sez6 antibody [14E5] ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sez6 antibody [14E5] ab252863).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24579.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling Sez6 with Anti-Sez6 antibody [14E5] ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the mouse hippocampus. The section was incubated with Anti-Sez6 antibody [14E5] ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sez6 antibody [14E5] ab252863).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-14e5-bsa-and-azide-free-ab255859--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42154.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [14E5] - BSA and Azide free (ab255859)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Sez6 with Anti-Sez6 antibody [14E5] ab252863 at 1/500 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining on the mouse cerebrum. The section was incubated with Anti-Sez6 antibody [14E5] ab252863 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Sez6 antibody [14E5] ab252863).
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