Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)

Rabbit Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for WB, IHC-P, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (AB314234), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	I-ELISA	IP	Flow Cyt	ICC/IF	IHC-Fr
Human	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Tested	Tested	Expected	Not recommended	Not recommended	Not recommended	Not recommended
Recombinant fragment - Human	Not recommended	Not recommended	Tested	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Recombinant fragment - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human, Recombinant fragment - Human	Dilution info -	Notes -

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

See full target information Seizure protein 6 homolog

Function

May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity (By similarity).

Alternative names

Seizure protein 6 homolog, SEZ-6, hSEZ-6, SEZ6

Recommended products

Rabbit Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for WB, IHC-P, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR28518-55
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab314234 is the carrier-free version of Anti-Sez6 antibody [EPR28518-55] ab314233.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Sez6 also known as seizure-related gene 6 protein plays an important role in the nervous system. It has a mass of about 110 kDa and is mostly expressed in the brain. Sez6 is part of a family of proteins that includes Sez6L and Sez6L2. This protein contains multiple complement control protein (CCP) domains which are important for its function in modulating neural activities.

Biological function summary

Sez6 modulates synaptic transmission by regulating AMPA receptor trafficking at the synapse. This protein participates in the complex of proteins involved in synaptic maintenance and plasticity. Through its effect on synaptic receptors Sez6 influences calcium influx and neurotransmitter release important processes for proper neural signaling and cognitive functions.

Pathways

Sez6 influences synaptic signaling and neuronal plasticity pathways. It particularly affects the glutamatergic signaling pathway altering the function of AMPA receptors. Sez6 interacts with other synaptic proteins like PSD-95 and glutamate receptors which mediate communication between neurons and adjust synaptic strength.

Associated diseases and disorders

Sez6 has been linked to neurodevelopmental disorders such as autism spectrum disorder. It is also associated with epilepsy as disruptions in synaptic regulation could lead to seizures. The connection to these disorders often involves altered interactions with proteins like NRXN and NLGN which are vital for synaptic formation and maintenance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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10 product images

Western blot - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
Sez6 Western blot staining using rabbit Anti-Sez6 antibody
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle, liver, spleen (PMID:7723619).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Sez6 antibody [EPR28518-55] (Anti-Sez6 antibody [EPR28518-55] ab314233) at 1/1000 dilution
Lane 1: Human hippocampus tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
Lane 3: Human liver tissue lysate at 20 µg
Lane 4: Human spleen tissue lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 170 kDa, 140 kDa
Exposure time: 70s
Western blot - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
Sez6 Western blot staining using rabbit Anti-Sez6 antibody
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: heart, lung, liver, spleen (PMID:7723619).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Sez6 antibody [EPR28518-55] (Anti-Sez6 antibody [EPR28518-55] ab314233) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse hippocampus tissue lysate at 20 µg
Lane 3: Mouse cerebellum tissue lysate at 20 µg
Lane 4: Mouse heart tissue lysate at 20 µg
Lane 5: Mouse lung tissue lysate at 20 µg
Lane 6: Mouse liver tissue lysate at 20 µg
Lane 7: Mouse spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 170 kDa, 140 kDa
Exposure time: 70s
Western blot - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
Sez6 Western blot staining using rabbit Anti-Sez6 antibody
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: skeletal muscle (PMID:7723619).
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Sez6 antibody [EPR28518-55] (Anti-Sez6 antibody [EPR28518-55] ab314233) at 1/1000 dilution
Lane 1: Rat brain tissue lysate at 20 µg
Lane 2: Rat cerebellum tissue lysate at 20 µg
Lane 3: Rat skeletal muscle tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 170 kDa, 140 kDa
Exposure time: 125s
Indirect ELISA - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Sez6 antibody [EPR28518-55] ab314233 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Sez6.
Antigen concentration: 1000 ng/ml
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human cardiac muscle. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse cerebrum. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human astrocytoma. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sez6 antibody [EPR28518-55] - BSA and Azide free (ab314234)
This data was developed using Anti-Sez6 antibody [EPR28518-55] ab314233, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-55] ab314233 at 1/500 (1.032 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum. The section was incubated with Anti-Sez6 antibody [EPR28518-55] ab314233 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.