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Rabbit Recombinant Monoclonal SEZ6 antibody. Suitable for IP, ICC/IF, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (AB314452), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (AB314452), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (AB314452), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (AB314452), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (AB314452), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPICC/IFIHC-FrI-ELISAIHC-PFlow Cyt
Human
Tested
Not recommended
Expected
Expected
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Expected
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Expected
Not recommended
Not recommended
Recombinant fragment - Human
Not recommended
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Human, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant fragment - Human
Dilution info
1000 ng/mL
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity (By similarity).

Alternative names

Seizure protein 6 homolog, SEZ-6, hSEZ-6, SEZ6

Recommended products

Rabbit Recombinant Monoclonal SEZ6 antibody. Suitable for IP, ICC/IF, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28518-83
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human ICC.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Sez6 also known as seizure-related gene 6 protein plays an important role in the nervous system. It has a mass of about 110 kDa and is mostly expressed in the brain. Sez6 is part of a family of proteins that includes Sez6L and Sez6L2. This protein contains multiple complement control protein (CCP) domains which are important for its function in modulating neural activities.

Biological function summary

Sez6 modulates synaptic transmission by regulating AMPA receptor trafficking at the synapse. This protein participates in the complex of proteins involved in synaptic maintenance and plasticity. Through its effect on synaptic receptors Sez6 influences calcium influx and neurotransmitter release important processes for proper neural signaling and cognitive functions.

Pathways

Sez6 influences synaptic signaling and neuronal plasticity pathways. It particularly affects the glutamatergic signaling pathway altering the function of AMPA receptors. Sez6 interacts with other synaptic proteins like PSD-95 and glutamate receptors which mediate communication between neurons and adjust synaptic strength.

Associated diseases and disorders

Sez6 has been linked to neurodevelopmental disorders such as autism spectrum disorder. It is also associated with epilepsy as disruptions in synaptic regulation could lead to seizures. The connection to these disorders often involves altered interactions with proteins like NRXN and NLGN which are vital for synaptic formation and maintenance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Sez6 Immunocytochemistry/ Immunofluorescence staining of mouse primary neuron using rabbit Anti-Sez6 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Sez6 Immunocytochemistry/ Immunofluorescence staining of rat primary neuron using rabbit Anti-Sez6 antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized rat primary neuron cells labelling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse heart (PMID: 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

  • Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Sez6 was immunoprecipitated from 0.35 mg Mouse hippocampus tissue lysate with ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Mouse hippocampus tissue lysate
    Lane 2: ab314452 IP in Mouse hippocampus tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314452 in mouse hippocampus tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    The IP experiment was performed by ab314452 using mouse hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (Anti-Sez6 antibody [EPR28518-55] ab314233)(1:1000 dilution).

    All lanes: Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452) at 1/30 dilution

    All lanes: Mouse hippocampus tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Sez6 was immunoprecipitated from 0.35 mg Human hippocampus tissue lysate with ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Human hippocampus tissue lysate
    Lane 2: ab314452 IP in Human hippocampus tissue lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314452 in human hippocampus tissue lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    The IP experiment was performed by ab314452 using human hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (Anti-Sez6 antibody [EPR28518-55] ab314233)(1:1000 dilution).

    All lanes: Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (ab314452) at 1/30 dilution

    All lanes: Human hippocampus tissue lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] (ab314452), expandable thumbnail

    Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] (ab314452)

    Indirect ELISA analysis of ab314452 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
    Antigen: Human Sez6.
    Antigen concentration: 1000 ng/ml

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Product protocols

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