Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Sez6 was immunoprecipitated from 0.35 mg Human hippocampus tissue lysate with ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Human hippocampus tissue lysate Lane 2 : ab314452 IP in Human hippocampus tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314452 in human hippocampus tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. The IP experiment was performed by ab314452 using human hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (ab314233)(1 : 1000 dilution).

All lanes:

Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (<a href='/en-us/products/primary-antibodies/sez6-antibody-epr28518-83-ab314452'>ab314452</a>) at 1/30 dilution

All lanes:

Human hippocampus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : confocal image showing no staining on mouse heart (PMID : 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized rat primary neuron cells labelling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Sez6 with ab314452 at 1/50 (9.88 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control : confocal image showing no staining on mouse liver (PMID : 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Sez6 was immunoprecipitated from 0.35 mg Mouse hippocampus tissue lysate with ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse hippocampus tissue lysate Lane 2 : ab314452 IP in Mouse hippocampus tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314452 in mouse hippocampus tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. The IP experiment was performed by ab314452 using mouse hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (ab314233)(1 : 1000 dilution).

All lanes:

Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (<a href='/en-us/products/primary-antibodies/sez6-antibody-epr28518-83-ab314452'>ab314452</a>) at 1/30 dilution

All lanes:

Mouse hippocampus tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)

This data was developed using ab314452, the same antibody clone in a different buffer formulation. Indirect ELISA analysis of ab314452 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution. Antigen : Human Sez6. Antigen concentration : 1000 ng/ml