Rabbit Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421153.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)")
![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421151.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)")
![Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunocytochemistry-immunofluorescence-img421139.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)")
![Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunocytochemistry-immunofluorescence-img421140.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)")
![Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunoprecipitation-img421051.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (AB314453)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ICC/IF | IHC-Fr | I-ELISA | IHC-P | Flow Cyt | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected | Not recommended | Not recommended |
| Mouse | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
| Rat | Expected | Tested | Expected | Expected | Not recommended | Not recommended |
| Recombinant fragment - Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
May play a role in cell-cell recognition and in neuronal membrane signaling. Seems to be important for the achievement of the necessary balance between dendrite elongation and branching during the elaboration of a complex dendritic arbor. Involved in the development of appropriate excitatory synaptic connectivity (By similarity).
Alternative names
Seizure protein 6 homolog, SEZ-6, hSEZ-6, SEZ6
Recommended products
Rabbit Recombinant Monoclonal SEZ6 antibody. Carrier free. Suitable for IP, ICC/IF, IHC-Fr, I-ELISA and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28518-83
- Purification technique
- Affinity purification Protein A
- Specificity
Unsuitable for human ICC.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314453 is the carrier-free version of Anti-Sez6 antibody [EPR28518-83] ab314452.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Sez6 also known as seizure-related gene 6 protein plays an important role in the nervous system. It has a mass of about 110 kDa and is mostly expressed in the brain. Sez6 is part of a family of proteins that includes Sez6L and Sez6L2. This protein contains multiple complement control protein (CCP) domains which are important for its function in modulating neural activities.
- Biological function summary
Sez6 modulates synaptic transmission by regulating AMPA receptor trafficking at the synapse. This protein participates in the complex of proteins involved in synaptic maintenance and plasticity. Through its effect on synaptic receptors Sez6 influences calcium influx and neurotransmitter release important processes for proper neural signaling and cognitive functions.
- Pathways
Sez6 influences synaptic signaling and neuronal plasticity pathways. It particularly affects the glutamatergic signaling pathway altering the function of AMPA receptors. Sez6 interacts with other synaptic proteins like PSD-95 and glutamate receptors which mediate communication between neurons and adjust synaptic strength.
- Associated diseases and disorders
Sez6 has been linked to neurodevelopmental disorders such as autism spectrum disorder. It is also associated with epilepsy as disruptions in synaptic regulation could lead to seizures. The connection to these disorders often involves altered interactions with proteins like NRXN and NLGN which are vital for synaptic formation and maintenance.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421153.jpg "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse heart (PMID: 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sez6 antibody [EPR28518-83] ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421151.jpg "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sez6 antibody [EPR28518-83] ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunocytochemistry-immunofluorescence-img421139.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse primary neuron cells labelling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.![Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunocytochemistry-immunofluorescence-img421140.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunocytochemistry/ Immunofluorescence - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized rat primary neuron cells labelling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain at 1/500 (4 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.![Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunoprecipitation-img421051.jpg "Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Sez6 was immunoprecipitated from 0.35 mg Mouse hippocampus tissue lysate with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse hippocampus tissue lysate
Lane 2: Anti-Sez6 antibody [EPR28518-83] ab314452 IP in Mouse hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Sez6 antibody [EPR28518-83] ab314452 in mouse hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The IP experiment was performed by Anti-Sez6 antibody [EPR28518-83] ab314452 using mouse hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (Anti-Sez6 antibody [EPR28518-55] ab314233)(1:1000 dilution).All lanes: Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (Anti-Sez6 antibody [EPR28518-83] ab314452) at 1/30 dilution
All lanes: Mouse hippocampus tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunoprecipitation-img421050.jpg "Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Sez6 was immunoprecipitated from 0.35 mg Human hippocampus tissue lysate with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human hippocampus tissue lysate
Lane 2: Anti-Sez6 antibody [EPR28518-83] ab314452 IP in Human hippocampus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Sez6 antibody [EPR28518-83] ab314452 in human hippocampus tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
The IP experiment was performed by Anti-Sez6 antibody [EPR28518-83] ab314452 using human hippocampus tissue lysate. The blot was probed by another anti-Sez6 antibody (Anti-Sez6 antibody [EPR28518-55] ab314233)(1:1000 dilution).All lanes: Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] (Anti-Sez6 antibody [EPR28518-83] ab314452) at 1/30 dilution
All lanes: Human hippocampus tissue lysate
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
![Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--indirect-elisa-img421171.jpg "Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-Sez6 antibody [EPR28518-83] ab314452 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
Antigen: Human Sez6.
Antigen concentration: 1000 ng/ml![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421152.jpg "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)
This data was developed using Anti-Sez6 antibody [EPR28518-83] ab314452, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Sez6 with Anti-Sez6 antibody [EPR28518-83] ab314452 at 1/50 (9.88 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 26698217). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-Sez6 antibody [EPR28518-83] ab314452 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
![Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunoprecipitation-img421050.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
![Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--indirect-elisa-img421171.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
![Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453), expandable thumbnail](https://content.abcam.com/products/images/sez6-antibody-epr28518-83-bsa-and-azide-free-ab314453--immunohistochemistry-frozen-sections-img421152.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Sez6 antibody [EPR28518-83] - BSA and Azide free (ab314453)")
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