Rabbit Polyclonal SF2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 30 publications.
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- Cells 12:2023The Fibrotic Effects of LINC00663 in Human Hepatic Stellate LX-2 Cells and in Bile Duct-Ligated Cholestasis Mice Are Mediated through the Splicing Factor 2-Fibronectin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Chu,Linan Bao,Yun Teng,Bo Yuan,Lijie Ma,Ying Liu,Hui KangPubMed 36672150
- Nucleic acids research 50:5493-55122022Exonic splicing code and protein binding sites for calcium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReuben J Pengelly,Dara Bakhtiar,Ivana Borovská,Jana Královičová,Igor VořechovskýPubMed 35474482
- Developmental cell 57:624-637.e42022SRSF1 governs progenitor-specific alternative splicing to maintain adult epithelial tissue homeostasis and renewal.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingsheng Yu,Oscar Cazares,Alison D Tang,Hyun-Yi Kim,Tomas Wald,Adya Verma,Qi Liu,Mary Helen Barcellos-Hoff,Stephen N Floor,Han-Sung Jung,Angela N Brooks,Ophir D KleinPubMed 35202586
- The Journal of biological chemistry 297:1013062021Phosphorylation regulates arginine-rich RNA-binding protein solubility and oligomerization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSean R Kundinger,Eric B Dammer,Luming Yin,Cheyenne Hurst,Sarah Shapley,Lingyan Ping,Sohail Khoshnevis,Homa Ghalei,Duc M Duong,Nicholas T SeyfriedPubMed 34673031
- Journal of translational medicine 19:2102021CCL21 activation of the MALAT1/SRSF1/mTOR axis underpins the development of gastric carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianmei Fu,Xiaohong Tan,Huaming Tang,Jijiang LiuPubMed 34001131
- Molecular therapy. Nucleic acids 23:1304-13222021Exosomal LINC00174 derived from vascular endothelial cells attenuates myocardial I/R injury via p53-mediated autophagy and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiang Su,Xiang-Wei Lv,Yu-Li Xu,Ru-Ping Cai,Ri-Xin Dai,Xi-Heng Yang,Wei-Kun Zhao,Bing-Hui KongPubMed 33717651
- Cell death & disease 12:1152021LINC01152 upregulates MAML2 expression to modulate the progression of glioblastoma multiforme via Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianheng Wu et. al.PubMed 33483471
- Cancer immunology research :2020Tumor cell-derived TGFβ1 Attenuates Antitumor Immune Activity of T cells via Regulation of PD-1 mRNA.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPengfei Wu et. al.PubMed 32999004
- Aging 12:17694-177122020Preclinical studies show using enzalutamide is less effective in docetaxel-pretreated than in docetaxel-naïve prostate cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangyi Lin,Fu-Ju Chou,Jieyang Lu,Wanying Lin,Matthew Truong,Hao Tian,Yin Sun,Jie Luo,Rachel Yang,Yuanjie Niu,Rosa Nadal,Emmanuel S Antonarakis,Carlos Cordon-Cardo,Deepak Sahasrabudhe,Chi-Ping Huang,Shuyuan Yeh,Gonghui Li,Chawnshang ChangPubMed 32920545
- Molecular biology reports 47:3031-30402020TIMP1 intron 3 retention is a marker of colon cancer progression controlled by hnRNPA1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarion Flodrops et. al.PubMed 32200451
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Chicken | Predicted | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted | Predicted |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Pig, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Pig, Zebrafish | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Pig, Zebrafish | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken, Pig, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
Alternative names
ASF, SF2, SF2P33, SFRS1, OK/SW-cl.3, SRSF1, Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1
Recommended products
Rabbit Polyclonal SF2 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 30 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab129108.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-SF2 antibody (ab38017)
SF2 is extensively phosphorylated on serine residues in the RS domain (SwissProt).
ab38017 is targeted against all isoforms of the SF2 protein.
All lanes: Western blot - Anti-SF2 antibody (ab38017) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Western blot - Jurkat whole cell lysate (Jurkat whole cell lysate ab7899) at 10 µg
Lane 3: Western blot - A-431 whole cell lysate (A-431 whole cell lysate ab7909) at 10 µg
Lane 4: Western blot - HEK-293 whole cell lysate (HEK-293 whole cell lysate ab7902) at 10 µg
Lane 5: Hep G2 whole cell lysate (ab7900) at 10 µg
Lane 6: MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody (ab38017)
ICC/IF image of ab38017 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38017, 5μg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
This image is courtesy of an anonymous customer reviewWestern blot - Anti-SF2 antibody (ab38017)
All lanes: Western blot - Anti-SF2 antibody (ab38017) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: 293T whole cell lysate at 20 µg
SecondaryAll lanes: Alexa Fluor® conjugated goat anti-rabbit antibody at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 28 kDa
Observed band size: 34 kDa, 50 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody (ab38017)
IHC image of SF2 staining in Human normal spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38017, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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