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Rabbit Recombinant Monoclonal SF2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.


Images

Western blot - Anti-SF2 antibody [EPR8239] (AB129108), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (AB129108), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] (AB129108), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (AB129108), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/800 - 1/1500
Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/800 - 1/1500
Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/800 - 1/1500
Notes

For unpurified use at 1/100 - 1/250.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Human
Dilution info
1/10000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/150 - 1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Alternative names

ASF, SF2, SF2P33, SFRS1, OK/SW-cl.3, SRSF1, Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1

Recommended products

Rabbit Recombinant Monoclonal SF2 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8239
Purification technique
Affinity purification Protein A
Dissociation constant
8 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SF2 also known as Splicing Factor 2 or Alternative Splicing Factor 1 (ASF1) is a prototypical member of the SR protein family involved in mRNA splicing. It is approximately 33 kDa in mass. SF2 is expressed ubiquitously in the nucleus of eukaryotic cells where it plays a central role in the regulation of constitutive and alternative splicing processes.

Biological function summary

SF2 acts as an important regulator of gene expression by facilitating the splicing of pre-mRNA participating in spliceosome assembly. It interacts with other splicing factors and RNA to form spliceosomal complexes essential for splicing fidelity and efficiency. The precise modulation of splicing by SF2 allows for the generation of diverse protein isoforms from a single gene contributing to protein diversity within the cell.

Pathways

SF2 integrates into the RNA splicing pathway and is critical for splice site selection. It interacts with proteins such as U1 snRNP and U2AF65 influencing their binding to pre-mRNA. SF2 also plays roles in other key pathways like mRNA transport and translation thereby linking the splicing machinery with nuclear export and protein synthesis processes.

Associated diseases and disorders

Abnormalities in SF2 levels or functions have been linked to various cancers and spinal muscular atrophy (SMA). Cancer cells often show altered SF2 expression leading to splicing anomalies that promote tumorigenesis. Additionally SF2's interaction with proteins like hnRNP A1 influences disease mechanisms as splicing factor dysregulation can disrupt normal cellular processes and facilitate the progression of disorders such as SMA.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Western blot - Anti-SF2 antibody [EPR8239] (ab129108)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-SF2 antibody [EPR8239] (ab129108) at 1/10000 dilution

    All lanes: Mouse spleen tissue at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] (ab129108)

    Intracellular Flow Cytometry analysis of HeLa cells labelling SF2 with purified ab129108 at a dilution of 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] (ab129108)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF2 with purified ab129108 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Counterstained with DAPI.

    Control - primary antibody (1/150), secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 an Alexa Fluor® 594-conjugate goat anti-mouse IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Western blot - Anti-SF2 antibody [EPR8239] (ab129108)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-SF2 antibody [EPR8239] (ab129108) at 1/10000 dilution

    Lane 1: HepG2 cell lysate at 20 µg

    Lane 2: Raji cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: 293T cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 28 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] (ab129108)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SF2 with unpurified ab129108 at 1/100.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SF2 antibody [EPR8239] (ab129108), expandable thumbnail

    OI-RD Scanning - Anti-SF2 antibody [EPR8239] (ab129108)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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