Anti-SF2 antibody [EPR8239] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SF2 with unpurified ab129108 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labelling SF2 with purified ab129108 at a dilution of 1/70 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour^® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling SF2 with purified ab129108 at 1/150. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Counterstained with DAPI.

Control - primary antibody (1/150), secondary antibody ab150120 an Alexa Fluor^® 594-conjugate goat anti-mouse IgG (1/500).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebral cortex tissue labelling SF2 with purified ab129108 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer, pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

• WB

Lab

Western blot - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SF2 antibody [EPR8239] (<a href='/en-us/products/primary-antibodies/sf2-antibody-epr8239-ab129108'>ab129108</a>) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

293T cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa

false

• WB

Lab

Western blot - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

This data was developed using ab129108, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SF2 antibody [EPR8239] (<a href='/en-us/products/primary-antibodies/sf2-antibody-epr8239-ab129108'>ab129108</a>) at 1/10000 dilution

All lanes:

Mouse spleen tissue at 20 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 28 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-SF2 antibody [EPR8239] - BSA and Azide free (AB248306)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.