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Rabbit Recombinant Monoclonal SF2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (AB248619), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8240] - BSA and Azide free (AB248619), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (AB248619), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Expected
Tested
Tested
Mouse
Predicted
Not recommended
Expected
Predicted
Predicted
Rat
Predicted
Not recommended
Expected
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
-
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Alternative names

ASF, SF2, SF2P33, SFRS1, OK/SW-cl.3, SRSF1, Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1

Recommended products

Rabbit Recombinant Monoclonal SF2 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR8240
Purification technique
Affinity purification Protein A
Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Dissociation constant
5.7 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248619 is the carrier-free version of Anti-SF2 antibody [EPR8240] ab133689.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SF2 also known as Splicing Factor 2 or Alternative Splicing Factor 1 (ASF1) is a prototypical member of the SR protein family involved in mRNA splicing. It is approximately 33 kDa in mass. SF2 is expressed ubiquitously in the nucleus of eukaryotic cells where it plays a central role in the regulation of constitutive and alternative splicing processes.

Biological function summary

SF2 acts as an important regulator of gene expression by facilitating the splicing of pre-mRNA participating in spliceosome assembly. It interacts with other splicing factors and RNA to form spliceosomal complexes essential for splicing fidelity and efficiency. The precise modulation of splicing by SF2 allows for the generation of diverse protein isoforms from a single gene contributing to protein diversity within the cell.

Pathways

SF2 integrates into the RNA splicing pathway and is critical for splice site selection. It interacts with proteins such as U1 snRNP and U2AF65 influencing their binding to pre-mRNA. SF2 also plays roles in other key pathways like mRNA transport and translation thereby linking the splicing machinery with nuclear export and protein synthesis processes.

Associated diseases and disorders

Abnormalities in SF2 levels or functions have been linked to various cancers and spinal muscular atrophy (SMA). Cancer cells often show altered SF2 expression leading to splicing anomalies that promote tumorigenesis. Additionally SF2's interaction with proteins like hnRNP A1 influences disease mechanisms as splicing factor dysregulation can disrupt normal cellular processes and facilitate the progression of disorders such as SMA.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue sections labeling SF2 with Purified Anti-SF2 antibody [EPR8240] ab133689 at 1/100 dilution (4.16 μg/ml). Heat mediated antigen retrieval was performed perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SF2 antibody [EPR8240] ab133689)

  • Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SF2 with purified Anti-SF2 antibody [EPR8240] ab133689 at 1/50 dilution (8.3 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti sf2 antibody epr8240 immunocytochemistry hela human)

  • Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SF2 antibody [EPR8240] - BSA and Azide free (ab248619)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling SF2 with purified Anti-SF2 antibody [EPR8240] ab133689 at 1/40 dilution (10μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SF2 antibody [EPR8240] ab133689)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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