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AB248195

Anti-SF3A1 antibody [EPR7666] - BSA and Azide free

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Rabbit Recombinant Monoclonal SF3A1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

SAP114, SF3A1, Splicing factor 3A subunit 1, SF3a120, Spliceosome-associated protein 114, SAP 114

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A1 antibody [EPR7666] - BSA and Azide free (AB248195)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A1 antibody [EPR7666] - BSA and Azide free (AB248195)

This data was developed using ab128868, the same antibody clone in a different buffer formulation.

ab128868, at a 1/50 dilution, staining SF3A1 in paraffin embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-SF3A1 antibody [EPR7666] - BSA and Azide free (AB248195)
  • WB

Unknown

Western blot - Anti-SF3A1 antibody [EPR7666] - BSA and Azide free (AB248195)

This data was developed using ab128868, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SF3A1 antibody [EPR7666] - BSA and Azide free (ab248195) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Lymph node lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7666

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab248195 is the carrier-free version of ab128868.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SF3A1 protein also known as SAP114 is a component of the spliceosomal subunit SF3a critical for pre-mRNA splicing. It is approximately 79 kDa in mass. SF3A1 is expressed in various tissues contributing to its broad functional role. The protein is central in assembling the active spliceosome complex facilitating the precise removal of introns from pre-mRNA through exon recognition. This mechanical role is vital for synthesizing mature mRNA molecules necessary for proper gene expression.
Biological function summary

SF3A1 operates as an integral part of the SF3a complex which also consists of SF3A2 and SF3A3. This complex associates with the U2 small nuclear ribonucleoprotein (snRNP) within the spliceosome. The role of SF3A1 in the assembly and stability of this complex is significant ensuring efficient splicing of pre-mRNA. By doing this SF3A1 contributes to the regulation of gene expression by allowing for the generation of multiple mRNA variants from single genes a process known as alternative splicing.

Pathways

SF3A1 plays an important role in the splicing of pre-mRNA an important step in the RNA processing pathway. This pathway ensures that non-coding regions are accurately removed and exons are joined to produce functional mRNA transcripts. SF3A1 works in conjunction with proteins like SF3B1 and U2AF both of which are also spliceosome components. Together these facilitate the selection of splice sites impacting the production of proteins within the cell by influencing mRNA variants that are translated.

SF3A1 alterations have been implicated in myelodysplastic syndromes (MDS) and certain cancers. Mutations or dysregulation of SF3A1 are associated with aberrant splicing patterns contributing to the pathology of these diseases. For instance in MDS SF3A1 mutations may cause ineffective hematopoiesis through improper mRNA processing. SF3A1 interacts with other spliceosomal proteins like U2AF1 where alterations can lead to similar splicing defects that exacerbate disease conditions. Understanding SF3A1's role in such pathologies opens prospects for therapeutic interventions targeting splicing mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006). Within the 17S U2 SnRNP complex, SF3A1 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed : 10882114, PubMed : 11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed : 29360106, PubMed : 30315277).
See full target information SF3A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com