Anti-SF3A3 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-SF3A3 antibody (AB224814)

SF3A3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224814 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab224814 at 0.4 μg/ml.

Lane 1 : ab224814 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SF3A3 antibody (ab224814)

Predicted band size: 59 kDa

false

• WB

Supplier Data

Western blot - Anti-SF3A3 antibody (AB224814)

All lanes:

Western blot - Anti-SF3A3 antibody (ab224814) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 59 kDa

true

Exposure time: 30s