AB249286

Anti-SF3A3 antibody [EPR10020] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SF3A3 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples.

View Alternative Names

SAP61, SF3A3, Splicing factor 3A subunit 3, SF3a60, Spliceosome-associated protein 61, SAP 61

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin-fixed, paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

ab156873 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

ab156873 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

ab156873 showing +ve staining in Human cervical carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

ab156873 showing +ve staining in Human normal colon.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

ab156873 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

This data was developed using ab156873, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SF3A3 antibody [EPR10020] (<a href='/en-us/products/primary-antibodies/sf3a3-antibody-epr10020-ab156873'>ab156873</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 59 kDa

false

Unconjugated

Anti-SF3A3 antibody [EPR10020]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10020

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249286 is the carrier-free version of ab156873.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3A3 also known as Splicing Factor 3A Subunit 3 plays an important role in pre-mRNA splicing. It has a mass of approximately 60 kDa. SF3A3 is a component of the splicing machinery and localizes predominantly to the nucleus where it is involved in the assembly of the spliceosome. The gene encoding SF3A3 is expressed in a wide range of tissues indicating its fundamental role in the splicing process across different cell types.

Biological function summary

SF3A3 is part of the U2 small nuclear ribonucleoprotein (snRNP) complex which is important for the second step of the splicing process. This complex recognizes the pre-mRNA branch site and participates in the assembly of the spliceosome a complex that excises introns and joins exons to produce mature mRNA. The activity of SF3A3 is essential for the accurate splicing of precursor mRNA transcripts impacting gene expression regulation.

Pathways

SF3A3 plays a role in the mRNA processing pathway and the spliceosome pathway. These pathways are vital for gene expression regulation and the generation of functional RNA products. Within the spliceosome pathway SF3A3 is associated with other splicing factors such as SF3B1 which also plays a role in splicing regulation and influences the assembly and function of the spliceosome complex.

SF3A3 has been linked to cancer and myelodysplastic syndromes. Mutations or dysregulation of SF3A3 and its associated splicing factors like SF3B1 can lead to aberrant splicing which may contribute to oncogenesis and disease progression. Understanding SF3A3's role in these disorders could provide insights into therapeutic targets for treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310, PubMed : 8022796). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed : 10882114, PubMed : 11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed : 29360106, PubMed : 30315277).

See full target information SF3A3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SF3A3 antibody [EPR10020] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Western blot - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (AB249286)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise