JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB317408

Anti-SF3A3 antibody [S06-3I7]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Monoclonal SF3A3 antibody. Suitable for WB and reacts with Human, Rat, Chinese hamster samples. Immunogen corresponding to Synthetic Peptide within Human SF3A3.

View Alternative Names

SAP61, SF3A3, Splicing factor 3A subunit 3, SF3a60, Spliceosome-associated protein 61, SAP 61

1 Images
Western blot - Anti-SF3A3 Antibody [S06-3I7] (AB317408)
  • WB

Supplier Data

Western blot - Anti-SF3A3 Antibody [S06-3I7] (AB317408)

All lanes:

Western blot - Anti-SF3A3 Antibody [S06-3I7] (ab317408) at 1/1000 dilution

Lane 1:

HeLa cell lysate

Lane 2:

CHO-K1 cell lysate

Lane 3:

C6 cell lysate

Lane 4:

Jurkat cell lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S06-3I7

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Chinese hamster

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SF3A3.

Q12874

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chinese hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3A3 also known as Splicing Factor 3A Subunit 3 plays an important role in pre-mRNA splicing. It has a mass of approximately 60 kDa. SF3A3 is a component of the splicing machinery and localizes predominantly to the nucleus where it is involved in the assembly of the spliceosome. The gene encoding SF3A3 is expressed in a wide range of tissues indicating its fundamental role in the splicing process across different cell types.
Biological function summary

SF3A3 is part of the U2 small nuclear ribonucleoprotein (snRNP) complex which is important for the second step of the splicing process. This complex recognizes the pre-mRNA branch site and participates in the assembly of the spliceosome a complex that excises introns and joins exons to produce mature mRNA. The activity of SF3A3 is essential for the accurate splicing of precursor mRNA transcripts impacting gene expression regulation.

Pathways

SF3A3 plays a role in the mRNA processing pathway and the spliceosome pathway. These pathways are vital for gene expression regulation and the generation of functional RNA products. Within the spliceosome pathway SF3A3 is associated with other splicing factors such as SF3B1 which also plays a role in splicing regulation and influences the assembly and function of the spliceosome complex.

SF3A3 has been linked to cancer and myelodysplastic syndromes. Mutations or dysregulation of SF3A3 and its associated splicing factors like SF3B1 can lead to aberrant splicing which may contribute to oncogenesis and disease progression. Understanding SF3A3's role in these disorders could provide insights into therapeutic targets for treatment strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310, PubMed : 8022796). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 10882114, PubMed : 11533230, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed : 10882114, PubMed : 11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed : 29360106, PubMed : 30315277).
See full target information SF3A3

Additional targets

Sf3a2
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB172634

Anti-SF3B1 antibody [EPR11986]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (AB172634)

  • 1
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB128868

Anti-SF3A1 antibody [EPR7666]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A1 antibody [EPR7666] (AB128868)

  • 0
  • 0
Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com