Name: Anti-SF3B1 antibody [EPR11986]
SKU: ab172634
Rating: 1 (1 reviews)

Rabbit Recombinant Monoclonal SF3B1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Related conjugates and formulations

ab202926
Conjugated
HRP Anti-SF3B1 antibody [EPR11986]
ab240170
Carrier free
Anti-SF3B1 antibody [EPR11986] - BSA and Azide free
ab320190
Conjugated
Alexa Fluor® 488 Anti-SF3B1 antibody [EPR11986]
ab320191
Conjugated
Alexa Fluor® 647 Anti-SF3B1 antibody [EPR11986]
ab320192
Conjugated
Alexa Fluor® 555 Anti-SF3B1 antibody [EPR11986]
ab320193
Conjugated
Alexa Fluor® 594 Anti-SF3B1 antibody [EPR11986]
ab320194
Conjugated
PE Anti-SF3B1 antibody [EPR11986]
ab320195
Conjugated
APC Anti-SF3B1 antibody [EPR11986]
ab321172
Conjugated
Alexa Fluor® 750 Anti-SF3B1 antibody [EPR11986]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (AB172634), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Not recommended	Tested	Tested	Tested
Mouse	Expected	Not recommended	Tested	Tested	Expected
Rat	Expected	Not recommended	Tested	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200 - 1/500	Notes -
Species Mouse	Dilution info 1/200	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

See full target information SF3B1

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:12234937, PubMed:27720643, PubMed:32494006, PubMed:34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed:12234937). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed:15146077, PubMed:33509932). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).

Alternative names

SAP155, SF3B1, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Spliceosome-associated protein 155, SF3b155, SAP 155

Recommended products

Rabbit Recombinant Monoclonal SF3B1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 14 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR11986
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SF3B1 also known as Splicing Factor 3B subunit 1 plays an important role in the mRNA splicing process. This protein participates in the splicing machinery as part of the spliceosome contributing to the removal of introns from pre-mRNA. SF3B1 has a molecular weight of approximately 160 kDa. It is ubiquitously expressed yet shows a high level of activity in tissues with rapid cell division such as the bone marrow and lymphatic tissue reflecting its central involvement in gene expression regulation.

Biological function summary

SF3B1 is an essential component of the spliceosomal complex specifically the U2 snRNP component. It helps establish the branchpoint recognition complex ensuring accurate RNA splicing necessary for producing functional mRNA molecules. This process is important for the expression of diverse protein-coding genes affecting multiple aspects of cellular function and identity. The protein interacts with other spliceosomal proteins like SF3A and U2AF to guide the precise removal of non-coding sequences enabling proper translation into proteins.

Pathways

SF3B1 is involved in the mRNA processing pathway and influences the cell cycle pathway. During mRNA processing SF3B1 aids in assembling the spliceosome facilitating the excision of introns. The protein interacts with components like SF3B2 in coordinating splicing with other RNA processing events. In the cell cycle pathway SF3B1 indirectly affects gene expression and stability influencing cell cycle progression and serving as a regulatory node by modulating the splicing of key regulators active during cell division.

Associated diseases and disorders

Several studies associate SF3B1 mutations with certain types of cancer including myelodysplastic syndrome and chronic lymphocytic leukemia. These mutations often result in aberrant splicing events leading to altered gene expression profiles that can promote tumorigenesis. Within these disease contexts SF3B1 abnormalities can influence interactions with related proteins like SRSF2 contributing to misregulated splicing patterns of genes that drive cancerous transformations and affect patient prognosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] (ab172634)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
All lanes: Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution
Lane 1: HeLa lysate at 10 µg
Lane 2: Jurkat lysate at 10 µg
Lane 3: K562 lysate at 10 µg
Lane 4: Ramos lysate at 10 µg
Predicted band size: 146 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] (ab172634)
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time:
Lane 1-4: 1 second
Lane 5-6: 10 seconds

In Western blot, anti- H3 antibody (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) loading control staining at 1/100000 dilution and anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

Exposure time:
Lane 1-3: 3.25 seconds
Lane 4: 15 seconds
All lanes: Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) nuclear fraction at 20 µg
Lane 2: HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg
Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) nuclear fraction at 20 µg
Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) cytoplasmic fraction at 20 µg
Lane 5: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) nuclear fraction at 20 µg
Lane 6: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cytoplasmic fraction at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 145 kDa
Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST

Exposure time:
Lane 1-3: 3.25 seconds
Lane 4: 15 seconds
All lanes: Western blot - Anti-SF3B1 antibody [EPR11986] (ab172634) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 4: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 146 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervical adenocarcinoma epithelial cell) labeling SF3B1 with ab172634 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody.

Confocal image showing nuclear staining in Hela cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] (ab172634)
Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling SF3B1 with ab172634 at 1/200 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody.

Confocal image showing nuclear staining in NIH/3T3 cell line.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).