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AB240170

Anti-SF3B1 antibody [EPR11986] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SF3B1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

SAP155, SF3B1, Splicing factor 3B subunit 1, Pre-mRNA-splicing factor SF3b 155 kDa subunit, Spliceosome-associated protein 155, SF3b155, SAP 155

8 Images
Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).  Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervical adenocarcinoma epithelial cell) labeling SF3B1 with ab172634 at 1/200 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody. Confocal image showing nuclear staining in Hela cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling SF3B1 (red) with ab172634 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue labeling SF3B1 with ab172634 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human colon tissue labeling SF3B1 with ab172634 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

Immunofluorescence analysis of HeLa cells labeling SF3B1 with ab172634 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).

Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).  Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) labeling SF3B1 with ab172634 at 1/200 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. Nuclear counterstain was DAPI. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain antibody. Confocal image showing nuclear staining in NIH/3T3 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Western blot - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • WB

Lab

Western blot - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).  Blocking/Diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1-4 : 1 second Lane 5-6 : 10 seconds In Western blot, anti- H3 antibody (ab176842) loading control staining at 1/100000 dilution and anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SF3B1 antibody [EPR11986] (<a href='/en-us/products/primary-antibodies/sf3b1-antibody-epr11986-ab172634'>ab172634</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) nuclear fraction at 20 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) cytoplasmic fraction at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) nuclear fraction at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) cytoplasmic fraction at 20 µg

Lane 5:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) nuclear fraction at 20 µg

Lane 6:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cytoplasmic fraction at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 145 kDa

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Western blot - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)
  • WB

Lab

Western blot - Anti-SF3B1 antibody [EPR11986] - BSA and Azide free (AB240170)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172634).  Blocking/Diluting buffer and concentration : 5% NFDM/TBST Exposure time : Lane 1-3 : 3.25 seconds Lane 4 : 15 seconds

All lanes:

Western blot - Anti-SF3B1 antibody [EPR11986] (<a href='/en-us/products/primary-antibodies/sf3b1-antibody-epr11986-ab172634'>ab172634</a>) at 1/1000 dilution

Lane 1:

RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 146 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11986

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

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Anti-SF3B1 antibody [EPR11987(B)]

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Immunocytochemistry/ Immunofluorescence - Anti-SF3B1 antibody [EPR11987(B)] (AB170854)

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

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Primary Antibodies

AB172614

Anti-U2AF35/U2AF1 antibody [EPR12649(B)]

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Immunocytochemistry/ Immunofluorescence - Anti-U2AF35/U2AF1 antibody [EPR12649(B)] (AB172614)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab240170 is the carrier-free version of ab172634.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3B1 also known as Splicing Factor 3B subunit 1 plays an important role in the mRNA splicing process. This protein participates in the splicing machinery as part of the spliceosome contributing to the removal of introns from pre-mRNA. SF3B1 has a molecular weight of approximately 160 kDa. It is ubiquitously expressed yet shows a high level of activity in tissues with rapid cell division such as the bone marrow and lymphatic tissue reflecting its central involvement in gene expression regulation.
Biological function summary

SF3B1 is an essential component of the spliceosomal complex specifically the U2 snRNP component. It helps establish the branchpoint recognition complex ensuring accurate RNA splicing necessary for producing functional mRNA molecules. This process is important for the expression of diverse protein-coding genes affecting multiple aspects of cellular function and identity. The protein interacts with other spliceosomal proteins like SF3A and U2AF to guide the precise removal of non-coding sequences enabling proper translation into proteins.

Pathways

SF3B1 is involved in the mRNA processing pathway and influences the cell cycle pathway. During mRNA processing SF3B1 aids in assembling the spliceosome facilitating the excision of introns. The protein interacts with components like SF3B2 in coordinating splicing with other RNA processing events. In the cell cycle pathway SF3B1 indirectly affects gene expression and stability influencing cell cycle progression and serving as a regulatory node by modulating the splicing of key regulators active during cell division.

Several studies associate SF3B1 mutations with certain types of cancer including myelodysplastic syndrome and chronic lymphocytic leukemia. These mutations often result in aberrant splicing events leading to altered gene expression profiles that can promote tumorigenesis. Within these disease contexts SF3B1 abnormalities can influence interactions with related proteins like SRSF2 contributing to misregulated splicing patterns of genes that drive cancerous transformations and affect patient prognosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 12234937, PubMed : 27720643, PubMed : 32494006, PubMed : 34822310, PubMed : 36104565). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 32494006, PubMed : 34822310, PubMed : 36104565). Within the 17S U2 SnRNP complex, SF3B1 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 36104565). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932). Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity).
See full target information SF3B1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 13:860898 PubMed35401227

2022

Perftoran Inhibits Hypoxia-Associated Resistance in Lung Cancer Cells to Carboplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Amira M Gamal-Eldeen,Amani A Alrehaili,Afaf Alharthi,Bassem M Raafat
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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