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AB56800

Anti-SF3B2 antibody [5D2]

0

(1 Review)

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(3 Publications)

Mouse Monoclonal SF3B2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SF3B2 aa 550-650.

View Alternative Names

SAP145, SF3B2, Splicing factor 3B subunit 2, Pre-mRNA-splicing factor SF3b 145 kDa subunit, Spliceosome-associated protein 145, SF3b145, SAP 145

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (AB56800)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B2 antibody [5D2] (AB56800)

SF3B2 antibody (ab56800) used in immunohistochemistry at 6ug/ml on formalin fixed and paraffin embedded human kidney.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (AB56800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SF3B2 antibody [5D2] (AB56800)

ab56800, at 60 μg/ml staining SF3B2 in HeLa Cells by immunocytochemistry. Immunofluorescence of ab56800 to SF3B2 on HeLa cell, performed with antibody concentration at 60 ug/ml.

This image was generated using the ascites version of the product.

Western blot - Anti-SF3B2 antibody [5D2] (AB56800)
  • WB

Unknown

Western blot - Anti-SF3B2 antibody [5D2] (AB56800)

SF3B2 antibody (ab56800) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-SF3B2 antibody [5D2] (ab56800)

Predicted band size: 98 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5D2

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SF3B2 aa 550-650. The exact immunogen used to generate this antibody is proprietary information.

Q13435

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SF3B2 protein also known as SAP145 is a component of the spliceosome complex important for RNA splicing. It has a significant molecular weight of approximately 145 kDa. SF3B2 expresses in various tissues throughout the human body indicating its importance in fundamental cellular activities. The protein mainly localizes to the nucleus where it interacts with other splicing factors to regulate the removal of introns from pre-mRNA.
Biological function summary

This protein plays a critical role in the pre-mRNA splicing process acting as part of the larger spliceosomal complex SF3b which is essential for accurate exon recognition and splice site selection. By ensuring the correct processing of genetic information SF3B2 influences gene expression and stability. It participates in the formation of the spliceosome's U2snRNP sub-complex which is necessary for splicing fidelity and overall cellular function.

Pathways

SF3B2 engages primarily in the mRNA splicing pathway which directly impacts gene expression and regulation. It associates with the splicing factor 3b complex which incorporates multiple proteins for efficient splicing. Its interaction with other spliceosomal proteins such as SF3B1 and U2AF facilitates the understanding of the exact splicing mechanism and highlights SF3B2's central role in these pathways.

Altered SF3B2 expression and function connect to cancer and myelodysplastic syndromes with SF3B1 mutations often observed in similar contexts. Researchers link spliceosomal dysfunctions caused by SF3B2 to aberrant splicing events contributing to oncogenesis and hematological disorders. As a result studies investigate SF3B2 as a potential target for therapeutic intervention focusing on restoring normal splicing activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006, PubMed : 34822310). Within the 17S U2 SnRNP complex, SF3B2 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).
See full target information SF3B2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 40:111266 PubMed36001976

2022

SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Simmler,Cédric Cortijo,Lisa Maria Koch,Patricia Galliker,Silvia Angori,Hella Anna Bolck,Christina Mueller,Ana Vukolic,Peter Mirtschink,Yann Christinat,Natalie R Davidson,Kjong-Van Lehmann,Giovanni Pellegrini,Chantal Pauli,Daniela Lenggenhager,Ilaria Guccini,Till Ringel,Christian Hirt,Kim Fabiano Marquart,Moritz Schaefer,Gunnar Rätsch,Matthias Peter,Holger Moch,Markus Stoffel,Gerald Schwank

The Journal of biological chemistry 293:10220-10234 PubMed29764937

2018

Cyclin-dependent kinase 1 (CDK1) and CDK2 have opposing roles in regulating interactions of splicing factor 3B1 with chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Tushar Murthy,Theresa Bluemn,Abhishek K Gupta,Michael Reimer,Sridhar Rao,Manoj M Pillai,Alex C Minella

The Journal of biological chemistry 288:355-67 PubMed23148228

2012

Re-evaluation of the role of calcium homeostasis endoplasmic reticulum protein (CHERP) in cellular calcium signaling.

Applications

WB

Species

Unspecified reactive species

Yaping Lin-Moshier,Peter J Sebastian,Leeann Higgins,Natalie D Sampson,Jane E Hewitt,Jonathan S Marchant
View all publications
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