Anti-SF3B3 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody (AB96683)

ab96683, at 1/100 dilution, staining SF3B3 in paraffin-embedded Saos-2 by Immunohistochemistry.

• WB

Unknown

Western blot - Anti-SF3B3 antibody (AB96683)

5% SDS-PAGE

All lanes:

Western blot - Anti-SF3B3 antibody (ab96683) at 1/3000 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 136 kDa

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• WB

CiteAb

Western blot - Anti-SF3B3 antibody (AB96683)

SF3B3 western blot using anti-SF3B3 antibody ab96683. Publication image and figure legend from Chakraborty, P., Huang, J. T. J., et al., 2018, Nat Commun, PubMed 30341290.

ab96683 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab96683 please see the product overview.

DHX9 helicase activity is required for R-loop formation and growth inhibition. a Expression of wild-type DHX9, but not a helicase-defective mutant (pGFP-DHX9dead), promotes R-loop formation in SFPQ-defective cells. Fluorescence intensity of S9.6 staining was measured for n > 50 cells. Statistical significance was determined using Mann–Whitney test (****p < 0.0001). b Expression of wild-type DHX9 but not a helicase-defective mutant confers impaired cell proliferation in SFPQ-depleted cells (siSFPQ8). Cell number was measured using a Casey Cell Counter 48 h after transfection with siRNA (time 0) and again after 3, 5, and 7 days. c RNA Pol II S2P was immunoprecipitated from Hela cell nuclear extracts and co-purification of different splicing factors was probed by western blot (as indicated). Duplicate samples were treated with RNaseA as indicated to demonstrate the requirement of RNA in these interactions

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