Anti-SF3B3 antibody [EPR18440]
- RabMAb
- Recombinant
- What is this?
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(8 Publications)
Rabbit Recombinant Monoclonal SF3B3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.
View Alternative Names
KIAA0017, SAP130, SF3B3, Splicing factor 3B subunit 3, Pre-mRNA-splicing factor SF3b 130 kDa subunit, STAF130, Spliceosome-associated protein 130, SF3b130, SAP 130
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SF3B3 with ab209402 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on Human colon is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SF3B3with ab209402 at 1/200 dilution (red) compared with a Rabbit IgG,monoclonal - Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunohistochemical analysis of paraffin-embedded Human breast cancer (ER+) tissue labeling SF3B3 with ab209402 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on ER+ breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunohistochemical analysis of paraffin-embedded Human breast cancer (ER-) tissue labeling SF3B3 with ab209402 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Weak nucleus staining on ER- breast cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling SF3B3 with ab209402 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on mouse cerebral cortex is observed. Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling SF3B3 with ab209402 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on RAW 264.7 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab209402 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling SF3B3 with ab209402 at 1/500 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on C6 cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (ab150120) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : ab209402 at 1/500 dilution followed by ab150120 at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3B3 antibody [EPR18440] (AB209402)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SF3B3 with ab209402 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Nucleus staining on rat kidney is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Lab
Western blot - Anti-SF3B3 antibody [EPR18440] (AB209402)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
We recommend not to boil the samples after lysis to get desired WB bands.
All lanes:
Western blot - Anti-SF3B3 antibody [EPR18440] (ab209402) at 1/10000 dilution
Lane 1:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates boiled at 20 µg
Lane 2:
HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates unboiled at 20 µg
Lane 3:
Jurkat (Human T cell leukemia T lymphocyte) whole lysate boiled at 20 µg
Lane 4:
Jurkat (Human T cell leukemia T lymphocyte) whole lysate unboiled at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution
Predicted band size: 136 kDa
Observed band size: 136 kDa
false
Exposure time: 5s
- WB
Supplier Data
Western blot - Anti-SF3B3 antibody [EPR18440] (AB209402)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-SF3B3 antibody [EPR18440] (ab209402) at 1/20000 dilution
Lane 1:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 136 kDa
Observed band size: 136 kDa
false
Exposure time: 8s
- WB
Supplier Data
Western blot - Anti-SF3B3 antibody [EPR18440] (AB209402)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1/2 : 4 seconds; Lane 3 : 1 second.
All lanes:
Western blot - Anti-SF3B3 antibody [EPR18440] (ab209402) at 1/2000 dilution
Lane 1:
Human fetal liver lysate at 10 µg
Lane 2:
Human fetal heart lysate at 10 µg
Lane 3:
Human fetal kidney lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 136 kDa
Observed band size: 136 kDa
false
- WB
Supplier Data
Western blot - Anti-SF3B3 antibody [EPR18440] (AB209402)
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : Lane 1/2/3 : 4 seconds : Lane 4/5/6/8/9 : 1 second; Lane 7 : 8 seconds.
All lanes:
Western blot - Anti-SF3B3 antibody [EPR18440] (ab209402) at 1/2000 dilution
Lane 1:
Mouse brain lysate at 10 µg
Lane 2:
Mouse heart lysate at 10 µg
Lane 3:
Mouse kidney lysate at 10 µg
Lane 4:
Mouse spleen lysate at 10 µg
Lane 5:
Rat brain lysate at 10 µg
Lane 6:
Rat kidney lysate at 10 µg
Lane 7:
Rat heart lysate at 10 µg
Lane 8:
C6 (Rat glial tumor cell line) whole cell lysate at 10 µg
Lane 9:
RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 136 kDa
Observed band size: 136 kDa
false
Related conjugates and formulations (1)
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Anti-SF3B3 antibody [EPR18440] - BSA and Azide free
Reactivity data
Product details
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SF3B3 participates in the assembly and stabilization of the functional spliceosomal complex. It is a part of the larger SF3b complex which is critical for recognizing the branch point sequence in RNA during splicing. This recognition influences the selection of splice sites and impacts the regulation of alternative splicing events. The SF3b complex works with the U2 small nuclear ribonucleoprotein (snRNP) during the splicing process to ensure accurate and efficient mRNA maturation.
Pathways
SF3B3 plays a significant role in the splicing pathway and impacts gene expression regulation through alternative splicing. It works alongside proteins such as SF3B1 and other splicing factors maintaining proper cellular function by influencing transcript variants. Another important pathway involving SF3B3 is RNA transport where it assists in the export of mature and correctly processed mRNA from the nucleus to the cytoplasm.
Product protocols
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Target data
Publications (8)
Recent publications for all applications. Explore the full list and refine your search
Journal of biochemical and molecular toxicology 38:e70062 PubMed39575578
2024
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eLife 13: PubMed39495216
2024
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Cancer gene therapy 30:375-387 PubMed36357564
2022
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Cells 10: PubMed33800128
2021
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Oncotarget 12:185-198 PubMed33613846
2021
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Journal of Crohn's & colitis : PubMed32333776
2020
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Annals of the New York Academy of Sciences 1462:128-138 PubMed31583714
2019
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Nature communications 8:1034 PubMed29044119
2017
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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