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AB183483

Anti-SF3B4 antibody [3A1]

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(2 Publications)

Mouse Monoclonal SF3B4 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SF3B4.

View Alternative Names

SAP49, SF3B4, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, Spliceosome-associated protein 49, SAP 49

1 Images
Western blot - Anti-SF3B4 antibody [3A1] (AB183483)
  • WB

Supplier Data

Western blot - Anti-SF3B4 antibody [3A1] (AB183483)

All lanes:

Western blot - Anti-SF3B4 antibody [3A1] (ab183483) at 1/2000 dilution

All lanes:

HeLa lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3A1

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SF3B4.

Q15427

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab183483 is affinity purified from tissue culture supernatant.
Storage buffer
Preservative: 0.06% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3B4 also known as SAP49 is a component of the spliceosome complex involved in pre-mRNA splicing. This protein weighs around 49 kDa and is expressed in various human tissues with especially high levels in the liver kidney and brain. SF3B4 functions by binding to pre-mRNA substrates and plays an essential role in recognizing splice sites an important step in the splicing process. SF3B4 along with other SF3B family members forms part of the U2 small nuclear ribonucleoprotein (snRNP) complex contributing to the accurate selection and removal of introns from pre-mRNA.
Biological function summary

SF3B4 participates in the precise editing and maturation of pre-mRNA during gene expression. As part of the U2 snRNP complex it collaborates with other factors in the assembly of the spliceosome. This complex ensures the precursor mRNA is properly spliced to produce mature mRNA closely linking SF3B4’s activity to efficient genetic information processing. SF3B4's function is especially important in ensuring the fidelity of the splicing mechanism which affects both mRNA stability and translational efficiency.

Pathways

The activity of SF3B4 is importantly tied to the RNA splicing and mRNA processing pathways. It interacts with other critical proteins like SF3B1 and SF3B2 which together facilitate the correct assembly and function of the spliceosome. This involvement in splicing pathways affects gene expression regulation an essential component of cellular differentiation and development. Disruption in SF3B4 or its associated proteins can lead to defective mRNA splicing impacting multiple downstream biological processes.

SF3B4’s function correlates with several splicing-related pathologies such as Nager syndrome and various cancers. Nager syndrome a rare congenital disorder involves mutations in SF3B4 that disrupt normal splicing processes. Additionally expression changes in SF3B4 have connections with oncogenesis and its interaction with other SF3B proteins contributes to aberrant splicing patterns often observed in cancerous cells. These interactions highlight its potential as a therapeutic target in disease treatment and management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006). Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).
See full target information SF3B4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:29 PubMed40045239

2025

CDC20 protects the heart from doxorubicin-induced cardiotoxicity by modulating CCDC69 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Feng,Ningning Zhang,Liang Wang,Xumin Guan,Yunpeng Xie,Yun-Long Xia

Bioengineered 13:2194-2206 PubMed35030977

2022

Enabled homolog (ENAH) regulated by RNA binding protein splicing factor 3b subunit 4 (SF3B4) exacerbates the proliferation, invasion and migration of hepatocellular carcinoma cells via Notch signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guoming Deng,Yufeng Luo,Yaoming Zhang,Jinfeng Zhang,Zongyun He
View all publications
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Product promise

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