AB157117

Anti-SF3B4 antibody

Name: Anti-SF3B4 antibody (ab157117) | Abcam
Brand: Abcam Limited
SKU: AB157117
Price: 535 USD
Availability: InStock

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(7 Publications)

Rabbit Polyclonal SF3B4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SF3B4 aa 350 to C-terminus.

View Alternative Names

SAP49, SF3B4, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, Spliceosome-associated protein 49, SAP 49

2 Images

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Immunoprecipitation - Anti-SF3B4 antibody (AB157117)

Immunoprecipitation analysis of whole cell lysate from 293T cells (1 mg for IP; 20% of IP loaded), labeling SF3B4 with ab157117 at 6 µg/mg lysate (lane 2). Immunoprecipitation of SF3B4 was also tested using an alternative Rabbit anti-SF3B4 (lane 1), or control IgG (lane 3). For blotting immunoprecipitated SF3B4, ab157117 was used at 1 µg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-SF3B4 antibody (ab157117)

Predicted band size: 44 kDa

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Western blot - Anti-SF3B4 antibody (AB157117)

All lanes:

Western blot - Anti-SF3B4 antibody (ab157117) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 44 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SF3B4 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15427

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SF3B4 also known as SAP49 is a component of the spliceosome complex involved in pre-mRNA splicing. This protein weighs around 49 kDa and is expressed in various human tissues with especially high levels in the liver kidney and brain. SF3B4 functions by binding to pre-mRNA substrates and plays an essential role in recognizing splice sites an important step in the splicing process. SF3B4 along with other SF3B family members forms part of the U2 small nuclear ribonucleoprotein (snRNP) complex contributing to the accurate selection and removal of introns from pre-mRNA.

Biological function summary

SF3B4 participates in the precise editing and maturation of pre-mRNA during gene expression. As part of the U2 snRNP complex it collaborates with other factors in the assembly of the spliceosome. This complex ensures the precursor mRNA is properly spliced to produce mature mRNA closely linking SF3B4’s activity to efficient genetic information processing. SF3B4's function is especially important in ensuring the fidelity of the splicing mechanism which affects both mRNA stability and translational efficiency.

Pathways

The activity of SF3B4 is importantly tied to the RNA splicing and mRNA processing pathways. It interacts with other critical proteins like SF3B1 and SF3B2 which together facilitate the correct assembly and function of the spliceosome. This involvement in splicing pathways affects gene expression regulation an essential component of cellular differentiation and development. Disruption in SF3B4 or its associated proteins can lead to defective mRNA splicing impacting multiple downstream biological processes.

SF3B4’s function correlates with several splicing-related pathologies such as Nager syndrome and various cancers. Nager syndrome a rare congenital disorder involves mutations in SF3B4 that disrupt normal splicing processes. Additionally expression changes in SF3B4 have connections with oncogenesis and its interaction with other SF3B proteins contributes to aberrant splicing patterns often observed in cancerous cells. These interactions highlight its potential as a therapeutic target in disease treatment and management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed : 10882114, PubMed : 12234937, PubMed : 27720643, PubMed : 32494006). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed : 12234937, PubMed : 32494006). Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA (PubMed : 12234937, PubMed : 27720643). Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed : 12234937). May also be involved in the assembly of the 'E' complex (PubMed : 10882114). Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs (PubMed : 15146077, PubMed : 33509932).

See full target information SF3B4

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 21:622-629 PubMed39467623

2024

SF3B4 Regulates Cellular Senescence and Suppresses Therapy-induced Senescence of Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seungyeon Yang,Minbeom Ko,Soojung Claire Hur,Eun Kyung Lee,Seung Min Jeong

Cell reports 40:111266 PubMed36001976

2022

SF3B1 facilitates HIF1-signaling and promotes malignancy in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Simmler,Cédric Cortijo,Lisa Maria Koch,Patricia Galliker,Silvia Angori,Hella Anna Bolck,Christina Mueller,Ana Vukolic,Peter Mirtschink,Yann Christinat,Natalie R Davidson,Kjong-Van Lehmann,Giovanni Pellegrini,Chantal Pauli,Daniela Lenggenhager,Ilaria Guccini,Till Ringel,Christian Hirt,Kim Fabiano Marquart,Moritz Schaefer,Gunnar Rätsch,Matthias Peter,Holger Moch,Markus Stoffel,Gerald Schwank

Molecules and cells 45:718-728 PubMed35996826

2022

SF3B4 Depletion Retards the Growth of A549 Non-Small Cell Lung Cancer Cells via UBE4B-Mediated Regulation of p53/p21 and p27 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hyungmin Kim,Jeehan Lee,Soon-Young Jung,Hye Hyeon Yun,Jeong-Heon Ko,Jeong-Hwa Lee

Nucleic acids research 50:8262-8278 PubMed35871302

2022

Cooperative engagement and subsequent selective displacement of SR proteins define the pre-mRNA 3D structural scaffold for early spliceosome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Kaushik Saha,Gourisankar Ghosh

Molecular cancer research : MCR 20:1574-1588 PubMed35852380

2022

Intronic Cis-Element DR8 in hTERT Is Bound by Splicing Factor SF3B4 and Regulates hTERT Splicing in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron L Slusher,Jeongjin J Kim,Mark Ribick,Jesse Pollens-Voigt,Armand Bankhead,Phillip L Palmbos,Andrew T Ludlow

Oncology letters 23:160 PubMed35399327

2022

GPAA1 promotes the proliferation, invasion and migration of hepatocellular carcinoma cells by binding to RNA-binding protein SF3B4.

Applications

Unspecified application

Species

Unspecified reactive species

Song Ge,Qiang Zhang,Xiaoyong Yang

Medicine and science in sports and exercise 54:931-943 PubMed35135999

2022

Acute Exercise Regulates hTERT Gene Expression and Alternative Splicing in the hTERT-BAC Transgenic Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron L Slusher,Jeongjin Jj Kim,Mark Ribick,Andrew T Ludlow

View all publications

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Product promise

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Anti-SF3B4 antibody

Immunoprecipitation - Anti-SF3B4 antibody (AB157117)

Western blot - Anti-SF3B4 antibody (AB157117)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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