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Mouse Monoclonal SFPQ antibody. Suitable for ICC, IP, WB, RIA, ICC/IF, AP, IHC-Fr and reacts with Mouse, Human samples. Cited in 27 publications. Immunogen corresponding to Cell preparation containing Sfpq protein.


Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Sfpq protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q8VIJ6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCIPWBRIAICC/IFAPIHC-Fr
Human
Expected
Expected
Expected
Expected
Expected
Expected
Expected
Mouse
Expected
Expected
Expected
Expected
Expected
Expected
Expected

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(see Lee et al reference).

Species
Human
Dilution info
-
Notes

(see Lee et al reference).

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(see Lee et al reference, Carramolino et al reference).

Species
Human
Dilution info
-
Notes

(see Lee et al reference, Carramolino et al reference).

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(See Lee et al reference, Shav-Tal et al reference).

Species
Human
Dilution info
-
Notes

(See Lee et al reference, Shav-Tal et al reference).

Expected
Expected

Species
Mouse
Dilution info
-
Notes

(see Lee et al reference).

Species
Human
Dilution info
-
Notes

(see Lee et al reference).

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

Target data

Function

DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA (PubMed:25765647). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Functions as a transcriptional activator (PubMed:25765647). Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as a transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF1-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation (By similarity). Required for the assembly of nuclear speckles (PubMed:25765647). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).

Alternative names

Recommended products

Mouse Monoclonal SFPQ antibody. Suitable for ICC, IP, WB, RIA, ICC/IF, AP, IHC-Fr and reacts with Mouse, Human samples. Cited in 27 publications. Immunogen corresponding to Cell preparation containing Sfpq protein.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Sfpq protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q8VIJ6
Clone number
B92
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SFPQ also known as splicing factor proline and glutamine-rich (PSF) is a multifunctional protein serving in various cellular processes. The protein weighs approximately 76 kDa and is ubiquitously expressed across different tissues. It mainly localizes in the nucleus where it interacts with RNA and other proteins. SFPQ plays essential roles in RNA splicing and transcription regulation among others.

Biological function summary

SFPQ takes part in multiple cellular processes including RNA processing gene expression regulation and DNA repair. SFPQ forms complexes with other proteins such as NONO which enhances its ability in transcriptional control and RNA splicing. By binding to DNA and RNA SFPQ acts as a bridge that connects various molecular events important for cell function and survival.

Pathways

The protein is actively involved in the modulation of RNA metabolic pathways and signal transduction. In particular SFPQ participates in the regulation of alternative splicing processes which can influence the diversity of the proteome. Functionally related proteins like FUS show interactions with SFPQ within these pathways jointly affecting gene expression dynamics.

Associated diseases and disorders

Aberrations in SFPQ expression or function associate with neurodegenerative diseases and cancers. Mislocalization of SFPQ and its altered expression have been observed in conditions like amyotrophic lateral sclerosis (ALS) and certain leukemia types. In these disorders proteins like TDP-43 have shown associations with SFPQ contributing to the pathology through disrupted RNA processing and cellular stress responses.

Product promise

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