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Rabbit Recombinant Monoclonal SFPQ antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat, Human samples.

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Images

Flow Cytometry - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358), expandable thumbnail
  • Western blot - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (AB246358), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Tested
Expected
Expected
Mouse
Tested
Expected
Expected
Tested
Rat
Tested
Expected
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

8 products for Alternative Version

Target data

Function

DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA (PubMed:25765647). The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Functions as transcriptional activator (PubMed:25765647). Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation (By similarity). Required for the assembly of nuclear speckles (PubMed:25765647). Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).

Alternative names

100 kDa DNA-pairing protein, Polypyrimidine tract-binding protein-associated-splicing factor, hPOMp100, PSF, PTB-associated-splicing factor, PSF, SFPQ

Recommended products

Rabbit Recombinant Monoclonal SFPQ antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Mouse, Rat, Human samples.

Alternative names

100 kDa DNA-pairing protein, Polypyrimidine tract-binding protein-associated-splicing factor, hPOMp100, PSF, PTB-associated-splicing factor, PSF, SFPQ

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR11847

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab246358 is the carrier-free version of Anti-SFPQ antibody [EPR11847] ab177149.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SFPQ also known as splicing factor proline and glutamine-rich (PSF) is a multifunctional protein serving in various cellular processes. The protein weighs approximately 76 kDa and is ubiquitously expressed across different tissues. It mainly localizes in the nucleus where it interacts with RNA and other proteins. SFPQ plays essential roles in RNA splicing and transcription regulation among others.

Biological function summary

SFPQ takes part in multiple cellular processes including RNA processing gene expression regulation and DNA repair. SFPQ forms complexes with other proteins such as NONO which enhances its ability in transcriptional control and RNA splicing. By binding to DNA and RNA SFPQ acts as a bridge that connects various molecular events important for cell function and survival.

Pathways

The protein is actively involved in the modulation of RNA metabolic pathways and signal transduction. In particular SFPQ participates in the regulation of alternative splicing processes which can influence the diversity of the proteome. Functionally related proteins like FUS show interactions with SFPQ within these pathways jointly affecting gene expression dynamics.

Associated diseases and disorders

Aberrations in SFPQ expression or function associate with neurodegenerative diseases and cancers. Mislocalization of SFPQ and its altered expression have been observed in conditions like amyotrophic lateral sclerosis (ALS) and certain leukemia types. In these disorders proteins like TDP-43 have shown associations with SFPQ contributing to the pathology through disrupted RNA processing and cellular stress responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Flow Cytometry - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    This data was developed using Anti-SFPQ antibody [EPR11847] ab177149, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling SFPQ with purified Anti-SFPQ antibody [EPR11847] ab177149 at 1/50 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Western blot - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    All lanes: Western blot - Anti-SFPQ antibody [EPR11847] (Anti-SFPQ antibody [EPR11847] ab177149) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lane 3: C2C12 (Mouse myoblasts myoblast) whole cell lysate

    Lane 4: Rat brain lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 76 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    This data was developed using Anti-SFPQ antibody [EPR11847] ab177149, the same antibody clone in a different buffer formulation.

    Immunocytochemistry analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling SFPQ with purified Anti-SFPQ antibody [EPR11847] ab177149 at 1/50 dilution (9.7 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    This data was developed using Anti-SFPQ antibody [EPR11847] ab177149, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling SFPQ with purified Anti-SFPQ antibody [EPR11847] ab177149 at 1/10000 dilution (0.05 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    This data was developed using Anti-SFPQ antibody [EPR11847] ab177149, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling SFPQ with purified Anti-SFPQ antibody [EPR11847] ab177149 at 1/10000 dilution (0.05 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFPQ antibody [EPR11847] - BSA and Azide free (ab246358)

    This data was developed using Anti-SFPQ antibody [EPR11847] ab177149, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue sections labeling SFPQ with purified Anti-SFPQ antibody [EPR11847] ab177149 at 1/10000 dilution (0.05 μg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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Product protocols

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