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Rabbit Recombinant Monoclonal SFRP1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

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Images

Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (AB267466), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (AB267466), expandable thumbnail
  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (AB267466), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (AB267466), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (AB267466), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Tested
Mouse
Tested
Tested
Not recommended
Not recommended
Expected
Rat
Expected
Tested
Not recommended
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

Alternative names

FRP, FRP1, SARP2, SFRP1, Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2

Recommended products

Rabbit Recombinant Monoclonal SFRP1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23092-253
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SFRP1 also known as Secreted Frizzled-Related Protein 1 is a protein that functions mechanically by modulating Wnt signaling pathways. This protein weighs approximately 35 kiloDaltons. SFRP1 acts as a modulator by binding to Wnt ligands preventing them from interacting with their cell surface receptors. It is expressed in various tissues including the kidney retina lung and heart.

Biological function summary

The secreted glycoprotein SFRP1 plays a role in cellular processes such as cell growth differentiation and apoptosis. As a member of the soluble frizzled-related proteins it acts to inhibit Wnt signaling pathways which are critical in maintaining cellular homeostasis. SFRP1 commonly interacts in the extracellular environment where it forms part of a regulatory complex that includes other extracellular matrix components to achieve its function.

Pathways

The role SFRP1 plays is important in the Wnt signaling pathway and the regulation of β-catenin activity. These pathways are involved in embryonic development and tissue regeneration. SFRP1 directly interacts with other proteins like frizzled receptors and Wnt ligands to maintain balance in signaling. This modulation further influences gene expression patterns and cellular behavior.

Associated diseases and disorders

An imbalance or alteration in SFRP1 activity is associated with cancer and osteoporosis. Its underexpression or lack of function can lead to abnormal cellular proliferation which is linked to tumorogenesis. In cancer SFRP1's interaction with the Wnt signaling pathway and its related proteins such as β-catenin highlights its role in maintaining normal cell function. Abnormal regulation also impacts bone density where SFRP1's regulatory function on Wnt S signaling may affect bone formation and lead to osteoporosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate with ab267466 at 1/30 dilution (2μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human kidney lysate 10μg

    Lane 2: ab267466 IP in human kidney lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267466 in human kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Predicted band size: 35 kDa

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

    Exposure time: 92 seconds.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

    Lane 1: Rat kidney lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab267466 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse kidney lysate 10μg

    Lane 2: ab267466 IP in mouse kidney lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267466 in mouse kidney lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 mins.

    All lanes: Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Predicted band size: 35 kDa

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).

    Negative control: MCF7 (PMID:15289865).

    Exposure time: 92 seconds.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

    Lane 1: Human breast lysate at 20 µg

    Lane 2: Human kidney lysate at 20 µg

    Lane 3: Human heart lysate at 20 µg

    Lane 4: SK-MEL-28 (human malignant melanoma) whole cell lysate at 20 µg

    Lane 5: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Mouse kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Exposure time: 114 seconds.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/5000 dilution

    All lanes: Recombinant human sFRP-1 protein, 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5μg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5μg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody.

    Negative control: MCF7 (PMID: 14968126).

  • Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466)

    ab267466 was shown to react with SFRP1 in wild-type A549 cells in Western blot with loss of signal observed in SFRP1 knockout cell line Human SFRP1 knockout A549 cell line ab277906. Wild-type A549 and SFRP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab267466 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Human SFRP1 knockout A549 cell line (Human SFRP1 knockout A549 cell line ab277906)

    Lane 1: Wild-type A549 lysate at 20 µg

    Lane 2: SFRP1 Knockout A549 lysate at 20 µg

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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