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Anti-SFRP1 antibody [EPR7003] (ab126613) is a rabbit monoclonal antibody that is used to detect SFRP1 in Western Blot, IHC-P. Suitable for Human samples.



- Specificity confirmed with SFRP1 knockout cell line validation


Images

Western blot - Anti-SFRP1 antibody [EPR7003] (AB126613), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (AB126613), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR7003] (AB126613), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR7003] (AB126613), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (AB126613), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

Alternative names

FRP, FRP1, SARP2, SFRP1, Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2

Recommended products

Anti-SFRP1 antibody [EPR7003] (ab126613) is a rabbit monoclonal antibody that is used to detect SFRP1 in Western Blot, IHC-P. Suitable for Human samples.



- Specificity confirmed with SFRP1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR7003
Purification technique
Affinity purification Protein A
Dissociation constant
3.01 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-SFRP1 antibody [EPR7003] (ab126613) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of SFRP1?


Anti-SFRP1 [EPR7003] (ab126613) specifically detects a band for SFRP1 (UniProt: Q8N474) at a molecular weight of 35kDa.

Trusted by the scientific community


Anti-SFRP1 [EPR7003] (ab126613) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-SFRP1 antibody [EPR7003] (ab126613) has been confirmed by Western blot testing in SFRP1 Knockout A549 cell line, Human SFRP1 knockout A549 cell line ab277906.



Other related products


We have a range of other formats of antibody clone [EPR7003] also available for your convenience:
ab126613, Carrier free - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free ab240023, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-SFRP1 antibody [EPR7003] ab320404, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-SFRP1 antibody [EPR7003] ab320405, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-SFRP1 antibody [EPR7003] ab320406, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-SFRP1 antibody [EPR7003] ab320407, PE - PE Anti-SFRP1 antibody [EPR7003] ab320408, APC - APC Anti-SFRP1 antibody [EPR7003] ab320409, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-SFRP1 antibody [EPR7003] ab321003



Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SFRP1 also known as Secreted Frizzled-Related Protein 1 is a protein that functions mechanically by modulating Wnt signaling pathways. This protein weighs approximately 35 kiloDaltons. SFRP1 acts as a modulator by binding to Wnt ligands preventing them from interacting with their cell surface receptors. It is expressed in various tissues including the kidney retina lung and heart.

Biological function summary

The secreted glycoprotein SFRP1 plays a role in cellular processes such as cell growth differentiation and apoptosis. As a member of the soluble frizzled-related proteins it acts to inhibit Wnt signaling pathways which are critical in maintaining cellular homeostasis. SFRP1 commonly interacts in the extracellular environment where it forms part of a regulatory complex that includes other extracellular matrix components to achieve its function.

Pathways

The role SFRP1 plays is important in the Wnt signaling pathway and the regulation of Β-catenin activity. These pathways are involved in embryonic development and tissue regeneration. SFRP1 directly interacts with other proteins like frizzled receptors and Wnt ligands to maintain balance in signaling. This modulation further influences gene expression patterns and cellular behavior.

Associated diseases and disorders

An imbalance or alteration in SFRP1 activity is associated with cancer and osteoporosis. Its underexpression or lack of function can lead to abnormal cellular proliferation which is linked to tumorogenesis. In cancer SFRP1's interaction with the Wnt signaling pathway and its related proteins such as Β-catenin highlights its role in maintaining normal cell function. Abnormal regulation also impacts bone density where SFRP1's regulatory function on Wnt S signaling may affect bone formation and lead to osteoporosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution

    All lanes: MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysates at 15 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/5000 dilution

    Lane 1: Human fetal kidney lysates at 15 µg

    Lane 2: Human fetal heart lysates at 15 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 36 kDa

  • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)

    All lanes: Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution

    Lane 1: Human heart lysate at 10 µg

    Lane 2: MDA-MB-435 lysate at 10 µg

    Lane 3: A375 lysate at 10 µg

    Secondary

    All lanes: Goat Anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 35 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] (ab126613)

    Unpurified ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    OI-RD Scanning - Anti-SFRP1 antibody [EPR7003] (ab126613)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613)

    ab126613 was shown to react with SFRP1 in wild-type A549 cells in Western blot with loss of signal observed in SFRP1 knockout cell line Human SFRP1 knockout A549 cell line ab277906. Wild-type A549 and SFRP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab126613 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR7003] (ab126613) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Human SFRP1 knockout A549 cell line (Human SFRP1 knockout A549 cell line ab277906)

    Lane 1: Wild-type A549 lysate at 20 µg

    Lane 2: SFRP1 Knockout A549 lysate at 20 µg

    Observed band size: 35 kDa

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Product protocols

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