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Knockout Tested Rabbit Recombinant Monoclonal SFRP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (AB240023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (AB240023), expandable thumbnail
  • OI-RD Scanning - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (AB240023), expandable thumbnail
  • Western blot - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (AB240023), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWB
Human
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

Alternative names

FRP, FRP1, SARP2, SFRP1, Secreted frizzled-related protein 1, FRP-1, sFRP-1, Secreted apoptosis-related protein 2, SARP-2

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal SFRP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7003
Purification technique
Affinity purification Protein A
Dissociation constant
3.01 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240023 is the carrier-free version of Anti-SFRP1 antibody [EPR7003] ab126613.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SFRP1 also known as Secreted Frizzled-Related Protein 1 is a protein that functions mechanically by modulating Wnt signaling pathways. This protein weighs approximately 35 kiloDaltons. SFRP1 acts as a modulator by binding to Wnt ligands preventing them from interacting with their cell surface receptors. It is expressed in various tissues including the kidney retina lung and heart.

Biological function summary

The secreted glycoprotein SFRP1 plays a role in cellular processes such as cell growth differentiation and apoptosis. As a member of the soluble frizzled-related proteins it acts to inhibit Wnt signaling pathways which are critical in maintaining cellular homeostasis. SFRP1 commonly interacts in the extracellular environment where it forms part of a regulatory complex that includes other extracellular matrix components to achieve its function.

Pathways

The role SFRP1 plays is important in the Wnt signaling pathway and the regulation of β-catenin activity. These pathways are involved in embryonic development and tissue regeneration. SFRP1 directly interacts with other proteins like frizzled receptors and Wnt ligands to maintain balance in signaling. This modulation further influences gene expression patterns and cellular behavior.

Associated diseases and disorders

An imbalance or alteration in SFRP1 activity is associated with cancer and osteoporosis. Its underexpression or lack of function can lead to abnormal cellular proliferation which is linked to tumorogenesis. In cancer SFRP1's interaction with the Wnt signaling pathway and its related proteins such as β-catenin highlights its role in maintaining normal cell function. Abnormal regulation also impacts bone density where SFRP1's regulatory function on Wnt S signaling may affect bone formation and lead to osteoporosis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling SFRP1 with Purified Anti-SFRP1 antibody [EPR7003] ab126613 at 1:50 dilution (8 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SFRP1 antibody [EPR7003] ab126613).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023)

    Unpurified Anti-SFRP1 antibody [EPR7003] ab126613, at 1/50 dilution, staining SFRP1 in Formalin-fixed, Paraffin-embedded Human heart muscle tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-SFRP1 antibody [EPR7003] ab126613).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023), expandable thumbnail

    OI-RD Scanning - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023), expandable thumbnail

    Western blot - Anti-SFRP1 antibody [EPR7003] - BSA and Azide free (ab240023)

    Anti-SFRP1 antibody [EPR7003] ab126613 was shown to react with SFRP1 in wild-type A549 cells in Western blot with loss of signal observed in SFRP1 knockout cell line Human SFRP1 knockout A549 cell line ab277906. Wild-type A549 and SFRP1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-SFRP1 antibody [EPR7003] ab126613 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-SFRP1 antibody [EPR7003] (Anti-SFRP1 antibody [EPR7003] ab126613) at 1/1000 dilution

    Lanes 1 - 2: Western blot - Human SFRP1 knockout A549 cell line (Human SFRP1 knockout A549 cell line ab277906)

    Lane 1: Wild-type A549 lysate at 20 µg

    Lane 2: SFRP1 Knockout A549 lysate at 20 µg

    Observed band size: 35 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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