Rabbit Polyclonal SFRP2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SFRP2 aa 200 to C-terminus.
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- Cancer discovery 12:2906-29292022Epigenome Programming by H3.3K27M Mutation Creates a Dependence of Pediatric Glioma on SMARCA4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Mo,Shoufu Duan,Xu Zhang,Xu Hua,Hui Zhou,Hong-Jian Wei,Jun Watanabe,Nicholas McQuillan,Zhenyi Su,Wei Gu,Cheng-Chia Wu,Christopher R Vakoc,Rintaro Hashizume,Kenneth Chang,Zhiguo ZhangPubMed 36305747
- PloS one 16:e02608642021Downregulation of SFRP2 facilitates cancer stemness and radioresistance of glioma cells via activating Wnt/β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuansheng Wu,Xiaofeng Yin,Wenbo Zhao,Wenli Xu,Laizhao ChenPubMed 34852024
- Developmental cell 56:95-110.e102020Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroaki Kasashima,Angeles Duran,Anxo Martinez-Ordoñez,Yuki Nakanishi,Hiroto Kinoshita,Juan F Linares,Miguel Reina-Campos,Yotaro Kudo,Antoine L'Hermitte,Masakazu Yashiro,Masaichi Ohira,Fei Bao,Daniele V F Tauriello,Eduard Batlle,Maria T Diaz-Meco,Jorge MoscatPubMed 33207226
- Clinical cancer research : an official journal of the American Association for Cancer Research 26:5709-57192020sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMitchell E Fane,Brett L Ecker,Amanpreet Kaur,Gloria E Marino,Gretchen M Alicea,Stephen M Douglass,Yash Chhabra,Marie R Webster,Andrea Marshall,Richard Colling,Olivia Espinosa,Nicholas Coupe,Neera Maroo,Leticia Campo,Mark R Middleton,Pippa Corrie,Xiaowei Xu,Giorgos C Karakousis,Ashani T WeeraratnaPubMed 33097493
- Science advances 6:eaba16852020Dermal exosomes containing miR-218-5p promote hair regeneration by regulating β-catenin signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiqi Hu et. al.PubMed 32832660
- Nature cell biology 22:289-2962020Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Montagner et. al.PubMed 32094692
- Journal of cellular biochemistry 120:18378-183872019Overexpression of LINC00261 inhibits non-small cell lung cancer cells progression by interacting with miR-522-3p and suppressing Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingli Shi et. al.PubMed 31190356
- BioMed research international 2019:87454872019Gossypol Promotes Wnt/-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinqian Liang et. al.PubMed 31139657
- Journal of molecular cell biology 10:302-3152018miR-221/222 activate the Wnt/β-catenin signaling to promote triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanhong Liu,Zifeng Wang,Zukai Liu,Shuo Shi,Zhaoran Zhang,Jiawei Zhang,Haifan LinPubMed 30053090
- Nature communications 8:158702017Simultaneous overactivation of Wnt/β-catenin and TGFβ signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunchao Cai et. al.PubMed 28627514
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SFRP2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96HF1
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocolusing 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Dog, Zebrafish | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Alternative names
FRP2, SARP1, FKSG12, UNQ361/PRO697, SFRP2, Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1
Recommended products
Rabbit Polyclonal SFRP2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SFRP2 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SFRP2 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96HF1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SFRP2 also known as Secreted Frizzled-Related Protein 2 is a protein that primarily functions as a modulator of the Wnt signaling pathway. It is a secreted glycoprotein with an approximate mass of 35 kDa. SFRP2 contains a cysteine-rich domain that is homologous to the Wnt-binding site of Frizzled proteins. This protein is widely expressed in various tissues including the heart lung and kidneys. Its primary role involves regulating the activity of Wnt proteins by direct binding which impacts cell signaling processes.
- Biological function summary
SFRP2 plays a significant role in the regulation of cellular proliferation migration and differentiation. It often functions as a part of extracellular signaling complexes influencing tissue development and repair. SFRP2 has been implicated in controlling the balance between cell growth and apoptosis particularly in stem cell environments and developing organ systems. Its activity impacts embryonic development contributing to the formation and patterning of tissues by modulating the Wnt signaling cascade.
- Pathways
Scientists have extensively studied SFRP2 in the context of the Wnt signaling and angiogenesis pathways. In the Wnt signaling pathway SFRP2 intervenes by sequestering Wnt ligands and preventing them from activating Frizzled receptors thereby modulating downstream β-catenin activities. The protein shows interaction with other Wnt pathway components like DKK1 which also modulates Wnt signaling. In the angiogenesis pathway SFRP2's involvement affects vascular formation and stability linking it to proteins such as VEGF a critical regulator of blood vessel growth.
- Associated diseases and disorders
Researchers have linked SFRP2 to cancer and cardiac conditions. In cancer abnormal SFRP2 expression has been associated with tumor progression and metastasis often through its interaction with other Wnt pathway proteins like β-catenin and DKK1 altering cell survival and division. In cardiac disorders altered levels of SFRP2 impact heart remodeling and fibrosis connecting to proteins such as TGF-β involved in fibrotic processes. Scientists continue to investigate SFRP2's role in these conditions to develop targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-SFRP2 antibody - C-terminal (ab137560)
12% SDS PAGE
All lanes: Western blot - Anti-SFRP2 antibody - C-terminal (ab137560) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: Hela whole cell lysate at 30 µg
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SFRP2 antibody - C-terminal (ab137560)
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling SFRP2 with ab137560 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP2 antibody - C-terminal (ab137560)
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labelling SFRP2 with ab137560 at 1/500 dilution.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-SFRP2 antibody - C-terminal (ab137560)
SFRP2 western blot using anti-SFRP2 antibody - C-terminal ab137560. Publication image and figure legend from Cai, J., Fang, L., et al., 2017, Nat Commun, PubMed 28627514.
ab137560 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137560 please see the product overview.
miR-128-3p targets Axin1, SFRP2, WIF1, SMURF2 and PP1c in NSCLC cells.(a) Targetscan tool showing schematic representation of putative binding sites for miR-128-3p in 3′-UTRs of Axin1, SFRP2, WIF1, SMURFS and PP1c. (b) WB analysis of the protein levels of Axin1, SFRP2, WIF1, SMURF2 and PP1c in the indicated cells. (c) By immunoprecipitation against Ago1, RIP analysis reveals the interaction of miR-128-3p with the 3′-UTRs of Axin1, SFRP2, WIF1, SMURF2 or PP1c mRNA to form miRNP complexes. IgG immunoprecipitation, as well as the interaction of miR-128-3p with GAPDH and 5s rRNA, were used as negative controls. (d) Luciferase assay of pGL3-Axin1-3′-UTR, pGL3-SFRP2-3′-UTR, pGL3-SMURF2-3′-UTR, pGL3-PP1c-3′-UTR or pGL3-WIF1-3′-UTR reporters in the indicated cells, co-transfected with increasing amounts (20 and 50 nM) of the indicated oligonucleotides. The sequence of the miR-128-3p mutant is shown. (e) Effects of restoredexpression of Axin1, SFRP2, WIF1, SMURFS and PP1c in miR-128-3p-overexpressing cells on luciferase activities of the TOP/FOP reporter and TGF-β reporter. (f) Effects of restored expression of miR-128-3p target genes on cell migration and self-renewal measured by Transwell migration assay and sphere formation assay, respectively, in the indicated NSCLC cells. Error bars represent mean±s.d. derived from three independent experiments. A two-tailed Student’s t-test was used for statistical analysis (*P<0.05, **P<0.01).
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