Anti-SFRP2 antibody - C-terminal

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SFRP2 antibody - C-terminal (AB137560)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling SFRP2 with ab137560 at 1/200 dilution. Lower image is merged with DNA probe.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP2 antibody - C-terminal (AB137560)

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labelling SFRP2 with ab137560 at 1/500 dilution.

• WB

Supplier Data

Western blot - Anti-SFRP2 antibody - C-terminal (AB137560)

Samples were separated by 12% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-SFRP2 antibody - C-terminal (ab137560) at 1/500 dilution

Lane 1:

NT2D1 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 33 kDa

false

• WB

CiteAb

Western blot - Anti-SFRP2 antibody - C-terminal (AB137560)

SFRP2 western blot using anti-SFRP2 antibody - C-terminal ab137560. Publication image and figure legend from Cai, J., Fang, L., et al., 2017, Nat Commun, PubMed 28627514.

ab137560 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab137560 please see the product overview.

miR-128-3p targets Axin1, SFRP2, WIF1, SMURF2 and PP1c in NSCLC cells.(a) Targetscan tool showing schematic representation of putative binding sites for miR-128-3p in 3'-UTRs of Axin1, SFRP2, WIF1, SMURFS and PP1c. (b) WB analysis of the protein levels of Axin1, SFRP2, WIF1, SMURF2 and PP1c in the indicated cells. (c) By immunoprecipitation against Ago1, RIP analysis reveals the interaction of miR-128-3p with the 3'-UTRs of Axin1, SFRP2, WIF1, SMURF2 or PP1c mRNA to form miRNP complexes. IgG immunoprecipitation, as well as the interaction of miR-128-3p with GAPDH and 5s rRNA, were used as negative controls. (d) Luciferase assay of pGL3-Axin1-3'-UTR, pGL3-SFRP2-3'-UTR, pGL3-SMURF2-3'-UTR, pGL3-PP1c-3'-UTR or pGL3-WIF1-3'-UTR reporters in the indicated cells, co-transfected with increasing amounts (20 and 50 nM) of the indicated oligonucleotides. The sequence of the miR-128-3p mutant is shown. (e) Effects of restoredexpression of Axin1, SFRP2, WIF1, SMURFS and PP1c in miR-128-3p-overexpressing cells on luciferase activities of the TOP/FOP reporter and TGF-β reporter. (f) Effects of restored expression of miR-128-3p target genes on cell migration and self-renewal measured by Transwell migration assay and sphere formation assay, respectively, in the indicated NSCLC cells. Error bars represent mean±s.d. derived from three independent experiments. A two-tailed Student's t-test was used for statistical analysis (*p<0.05, **p<0.01).

false