Rabbit Recombinant Monoclonal SFRP2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Human samples.
Images
![Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186), expandable thumbnail](https://content.abcam.com/products/images/sfrp2-antibody-epr26088-157-bsa-and-azide-free-ab318186--dot-blot-img432856.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)")
![Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186), expandable thumbnail](https://content.abcam.com/products/images/sfrp2-antibody-epr26088-157-bsa-and-azide-free-ab318186--western-blot-img432800.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | WB | IHC-P | IP | |
|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Recombinant full length protein - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant full length protein - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.
Alternative names
FRP2, SARP1, FKSG12, UNQ361/PRO697, UNQ361/PRO697, FKSG12, SARP1, FRP2, SFRP2, Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1
Recommended products
Rabbit Recombinant Monoclonal SFRP2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR26088-157
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
Notes
ab318186 is the carrier-free version of Anti-SFRP2 antibody [EPR26088-157] ab318185.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SFRP2 also known as Secreted Frizzled-Related Protein 2 is a protein that primarily functions as a modulator of the Wnt signaling pathway. It is a secreted glycoprotein with an approximate mass of 35 kDa. SFRP2 contains a cysteine-rich domain that is homologous to the Wnt-binding site of Frizzled proteins. This protein is widely expressed in various tissues including the heart lung and kidneys. Its primary role involves regulating the activity of Wnt proteins by direct binding which impacts cell signaling processes.
- Biological function summary
SFRP2 plays a significant role in the regulation of cellular proliferation migration and differentiation. It often functions as a part of extracellular signaling complexes influencing tissue development and repair. SFRP2 has been implicated in controlling the balance between cell growth and apoptosis particularly in stem cell environments and developing organ systems. Its activity impacts embryonic development contributing to the formation and patterning of tissues by modulating the Wnt signaling cascade.
- Pathways
Scientists have extensively studied SFRP2 in the context of the Wnt signaling and angiogenesis pathways. In the Wnt signaling pathway SFRP2 intervenes by sequestering Wnt ligands and preventing them from activating Frizzled receptors thereby modulating downstream β-catenin activities. The protein shows interaction with other Wnt pathway components like DKK1 which also modulates Wnt signaling. In the angiogenesis pathway SFRP2's involvement affects vascular formation and stability linking it to proteins such as VEGF a critical regulator of blood vessel growth.
- Associated diseases and disorders
Researchers have linked SFRP2 to cancer and cardiac conditions. In cancer abnormal SFRP2 expression has been associated with tumor progression and metastasis often through its interaction with other Wnt pathway proteins like β-catenin and DKK1 altering cell survival and division. In cardiac disorders altered levels of SFRP2 impact heart remodeling and fibrosis connecting to proteins such as TGF-β involved in fibrotic processes. Scientists continue to investigate SFRP2's role in these conditions to develop targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186), expandable thumbnail](https://content.abcam.com/products/images/sfrp2-antibody-epr26088-157-bsa-and-azide-free-ab318186--dot-blot-img432856.jpg "Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186)")
Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186)
This data was developed using Anti-SFRP2 antibody [EPR26088-157] ab318185, the same antibody clone in a different buffer formulation.
Dot blot analysis of SFRP2 using Anti-SFRP2 antibody [EPR26088-157] ab318185 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: His-tagged human SFRP2 recombinant protein
Lane 2: His-tagged human SFRP1 recombinant proteinAnti-6X His tag ® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) (1:1000) as total protein control.
This antibody does not cross-react with human SFRP1.
All lanes: Dot Blot - Anti-SFRP2 antibody [EPR26088-157] (Anti-SFRP2 antibody [EPR26088-157] ab318185) at 1/1000 dilution
Lane 1: His-tagged human SFRP2 recombinant protein
Lane 2: His-tagged human SFRP1 recombinant protein
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Dot blot analysis of SFRP2 using Anti-SFRP2 antibody [EPR26088-157] ab318185 at 1:1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Lane 1: His-tagged human SFRP2 recombinant protein
Lane 2: His-tagged human SFRP1 recombinant proteinAnti-6X His tag ® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) (1:1000) as total protein control.
This antibody does not cross-react with human SFRP1.
![Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186), expandable thumbnail](https://content.abcam.com/products/images/sfrp2-antibody-epr26088-157-bsa-and-azide-free-ab318186--western-blot-img432800.jpg "Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186)")
Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (ab318186)
This data was developed using Anti-SFRP2 antibody [EPR26088-157] ab318185, the same antibody clone in a different buffer formulation.
Low expression: spleen.
Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-SFRP2 antibody [EPR26088-157] (Anti-SFRP2 antibody [EPR26088-157] ab318185) at 1/1000 dilution
Lane 1: Human eyeball tissue lysate at 30 µg
Lane 2: Human breast tissue lysate at 30 µg
Lane 3: Human spleen tissue lysate at 30 µg
Lane 4: Untreated Y79 (human retinoblastoma cell) whole cell lysate at 30 µg
Lane 5: Y79 treated with 300ng/ml Brefeldin A for 24 hours whole cell lysate at 30 µg
SecondaryLanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 4 - 5: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 35 kDa, 36 kDa
Exposure time: 180s
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