AB318186

Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal SFRP2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Human samples.

View Alternative Names

FRP2, SARP1, FKSG12, UNQ361/PRO697, SFRP2, Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1

2 Images

Supplier Data

Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

This data was developed using ab318185, the same antibody clone in a different buffer formulation.

Low expression : spleen.

Lanes 1-3 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 and lanes 4-5 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SFRP2 antibody [EPR26088-157] (<a href='/en-us/products/primary-antibodies/sfrp2-antibody-epr26088-157-ab318185'>ab318185</a>) at 1/1000 dilution

Lane 1:

Human eyeball tissue lysate at 30 µg with NFDM/TBST

Lane 2:

Human breast tissue lysate at 30 µg with NFDM/TBST

Lane 3:

Human spleen tissue lysate at 30 µg with NFDM/TBST

Lane 4:

Untreated Y79 (human retinoblastoma cell) whole cell lysate at 30 µg with NFDM/TBST

Lane 5:

Y79 treated with 300ng/ml Brefeldin A for 24 hours whole cell lysate at 30 µg with NFDM/TBST

Secondary

Lanes 1 - 3:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Lanes 4 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

This data was developed using ab318185, the same antibody clone in a different buffer formulation.

Dot blot analysis of SFRP2 using ab318185 at 1 : 1000 (0.501 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lane 1 : His-tagged human SFRP2 recombinant protein
Lane 2 : His-tagged human SFRP1 recombinant protein

Anti-6X His tag ® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 1000) as total protein control.

This antibody does not cross-react with human SFRP1.

All lanes:

Dot Blot - Anti-SFRP2 antibody [EPR26088-157] (<a href='/en-us/products/primary-antibodies/sfrp2-antibody-epr26088-157-ab318185'>ab318185</a>) at 1/1000 dilution

Lane 1:

His-tagged human SFRP2 recombinant protein

Lane 2:

His-tagged human SFRP1 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Unconjugated

Anti-SFRP2 antibody [EPR26088-157]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26088-157

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab318186 is the carrier-free version of ab318185.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRP2 also known as Secreted Frizzled-Related Protein 2 is a protein that primarily functions as a modulator of the Wnt signaling pathway. It is a secreted glycoprotein with an approximate mass of 35 kDa. SFRP2 contains a cysteine-rich domain that is homologous to the Wnt-binding site of Frizzled proteins. This protein is widely expressed in various tissues including the heart lung and kidneys. Its primary role involves regulating the activity of Wnt proteins by direct binding which impacts cell signaling processes.

Biological function summary

SFRP2 plays a significant role in the regulation of cellular proliferation migration and differentiation. It often functions as a part of extracellular signaling complexes influencing tissue development and repair. SFRP2 has been implicated in controlling the balance between cell growth and apoptosis particularly in stem cell environments and developing organ systems. Its activity impacts embryonic development contributing to the formation and patterning of tissues by modulating the Wnt signaling cascade.

Pathways

Scientists have extensively studied SFRP2 in the context of the Wnt signaling and angiogenesis pathways. In the Wnt signaling pathway SFRP2 intervenes by sequestering Wnt ligands and preventing them from activating Frizzled receptors thereby modulating downstream β-catenin activities. The protein shows interaction with other Wnt pathway components like DKK1 which also modulates Wnt signaling. In the angiogenesis pathway SFRP2's involvement affects vascular formation and stability linking it to proteins such as VEGF a critical regulator of blood vessel growth.

Researchers have linked SFRP2 to cancer and cardiac conditions. In cancer abnormal SFRP2 expression has been associated with tumor progression and metastasis often through its interaction with other Wnt pathway proteins like β-catenin and DKK1 altering cell survival and division. In cardiac disorders altered levels of SFRP2 impact heart remodeling and fibrosis connecting to proteins such as TGF-β involved in fibrotic processes. Scientists continue to investigate SFRP2's role in these conditions to develop targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

See full target information SFRP2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free

Western blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

Dot Blot - Anti-SFRP2 antibody [EPR26088-157] - BSA and Azide free (AB318186)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise