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AB285112

Anti-SFRP4 antibody [EPR23591-229] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal SFRP4 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

FRPHE, SFRP4, Secreted frizzled-related protein 4, sFRP-4, FrpHE

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Sandwich ELISA - Anti-SFRP4 antibody [EPR23591-229] - BSA and Azide free (Detector) (AB285112)
  • sELISA

Lab

Sandwich ELISA - Anti-SFRP4 antibody [EPR23591-229] - BSA and Azide free (Detector) (AB285112)

Sandwich ELISA of ab253839 with the capture antibody ab285006 dilution at 2 µg/mL and detector antibody ab285112 dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23591-229

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab285112 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human SFRP4. The recommended pair for sandwich ELISA is:
Capture: ab285006, Human SFRP4 Capture Antibody (unconjugated)
Detector: ab285112, Human SFRP4 Detector Antibody (unconjugated)
The reference range value is 0.31 - 20 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRP4 also known as Secreted Frizzled Related Protein 4 is a 40 kDa protein involved in modulating Wnt signaling pathways. It is a member of the secreted frizzled-related proteins (sFRPs) that act as Wnt antagonists. SFRP4 has a cysteine-rich domain homologous to the Wnt-binding site on frizzled proteins. Expressed in many tissues including the heart lung and kidney SFRP4 plays a role in various physiological processes by influencing cell signaling.
Biological function summary

SFRP4 regulates cell growth and differentiation. It interacts with Wnt ligands preventing them from binding to their receptors. SFRP4 does not typically function as part of a protein complex acting instead as an antagonist on its own. By inhibiting Wnt activity SFRP4 influences embryonic development tissue homeostasis and even cancer progression. The impact of SFRP4 varies depending on the tissue-specific context reflecting its complex biological roles.

Pathways

SFRP4 is significant in the Wnt signaling pathway and the β-catenin independent pathway. By interacting with Wnt ligands SFRP4 can prevent the activation of canonical Wnt/β-catenin signaling a pathway involved in cell fate determination and stem cell renewal. It also influences the non-canonical pathways where the Wnt signaling does not involve β-catenin impacting processes like cell movement and polarity. In these pathways SFRP4 interacts with proteins such as Disheveled and Axin modulating their activities.

The disruption of SFRP4 expression links to osteoarthritis and breast cancer. Abnormal SFRP4 levels can result in improper Wnt signaling contributing to these conditions. In osteoarthritis SFRP4 imbalance affects cartilage integrity while in breast cancer altered SFRP4 expression may influence tumor growth and progression. Related proteins such as β-catenin in cancer or MMPs in osteoarthritis highlight the importance of SFRP4 in disease susceptibility and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed : 12952927).
See full target information SFRP4
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Product promise

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