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AB154167

Anti-SFRP4 antibody [EPR9389]

3

(1 Review)

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(24 Publications)

Rabbit Recombinant Monoclonal SFRP4 antibody. Suitable for ICC/IF, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 24 publications.

View Alternative Names

FRPHE, SFRP4, Secreted frizzled-related protein 4, sFRP-4, FrpHE

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (AB154167)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (AB154167)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SFRP4 with ab154167 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SFRP4 antibody [EPR9389] (AB154167)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SFRP4 antibody [EPR9389] (AB154167)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling SFRP4 with unpurified ab154167 at 1/130 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (AB154167)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP4 antibody [EPR9389] (AB154167)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SFRP4 with ab154167 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SFRP4 antibody [EPR9389] (AB154167)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SFRP4 antibody [EPR9389] (AB154167)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling SFRP4 with ab154167 at 1/500 (2.722 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue). Confocal image showing positive staining in A549 cell line. Negative control : T-47D. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Immunocytochemistry/ Immunofluorescence - Anti-SFRP4 antibody [EPR9389] (AB154167)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-SFRP4 antibody [EPR9389] (AB154167)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling SFRP4 with ab154167 at 1/200 (6.805 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed seondary antibody used at 1/1000 diluting (2 µg/ml)(Green). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was (Blue). Confocal image showing positive staining in RAW 264.7 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9389

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRP4 also known as Secreted Frizzled Related Protein 4 is a 40 kDa protein involved in modulating Wnt signaling pathways. It is a member of the secreted frizzled-related proteins (sFRPs) that act as Wnt antagonists. SFRP4 has a cysteine-rich domain homologous to the Wnt-binding site on frizzled proteins. Expressed in many tissues including the heart lung and kidney SFRP4 plays a role in various physiological processes by influencing cell signaling.
Biological function summary

SFRP4 regulates cell growth and differentiation. It interacts with Wnt ligands preventing them from binding to their receptors. SFRP4 does not typically function as part of a protein complex acting instead as an antagonist on its own. By inhibiting Wnt activity SFRP4 influences embryonic development tissue homeostasis and even cancer progression. The impact of SFRP4 varies depending on the tissue-specific context reflecting its complex biological roles.

Pathways

SFRP4 is significant in the Wnt signaling pathway and the β-catenin independent pathway. By interacting with Wnt ligands SFRP4 can prevent the activation of canonical Wnt/β-catenin signaling a pathway involved in cell fate determination and stem cell renewal. It also influences the non-canonical pathways where the Wnt signaling does not involve β-catenin impacting processes like cell movement and polarity. In these pathways SFRP4 interacts with proteins such as Disheveled and Axin modulating their activities.

The disruption of SFRP4 expression links to osteoarthritis and breast cancer. Abnormal SFRP4 levels can result in improper Wnt signaling contributing to these conditions. In osteoarthritis SFRP4 imbalance affects cartilage integrity while in breast cancer altered SFRP4 expression may influence tumor growth and progression. Related proteins such as β-catenin in cancer or MMPs in osteoarthritis highlight the importance of SFRP4 in disease susceptibility and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types (By similarity). SFRP4 plays a role in bone morphogenesis. May also act as a regulator of adult uterine morphology and function. May also increase apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling (By similarity). Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake (PubMed : 12952927).
See full target information SFRP4
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Publications (24)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 116:2774-2787 PubMed40706632

2025

Single-Cell Transcription Reveals the Fibroblast Heterogeneity and Neural Cells' Significance in Desmoid Fibromatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junfeng Wang,Cheng Zhou,Peng Huang,Haodong Li,Ping Xu,Yiming Zheng,Dahui Wang,Bo Ning

International journal of molecular sciences 26: PubMed40565036

2025

PKA-Mediated Phosphorylation of SFRP4 Promotes Wnt/β-Catenin Activation and Cancer Stemness in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yoo-Lim Jhe,Suji Lee,Youjin Jung,Jae-Ho Cheong

Nature medicine 31:502-513 PubMed39775038

2025

Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Muñoz-Blat,Raúl Pérez-Moraga,Nerea Castillo-Marco,Teresa Cordero,Ana Ochando,Sheila Ortega-Sanchís,Marcos Parras-Moltó,Rogelio Monfort-Ortiz,Elena Satorres-Perez,Blanca Novillo,Alfredo Perales,Matthew Gormley,Sofia Granados-Aparici,Rosa Noguera,Beatriz Roson,Susan J Fisher,Carlos Simón,Tamara Garrido-Gómez

Heliyon 10:e37168 PubMed39286067

2024

Multi-omic analyses identified SFRP4 as a novel biomarker in abnormal uterine bleeding with ovulatory dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxiu Zhao,Yifei Lv,Yizhou Huang,Tao Zhang,Yibing Lan,Chunming Li,Peiqiong Chen,Wenxian Xu,Linjuan Ma,Jianhong Zhou

Frontiers in molecular neuroscience 17:1398872 PubMed38993819

2024

protein expression is reduced in high grade astrocytomas which is not caused by the methylation of its promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Anja Kafka,Nives Pećina-Šlaus,Denis Drmić,Anja Bukovac,Niko Njirić,Kamelija Žarković,Antonia Jakovčević

Nature communications 15:1384 PubMed38360902

2024

Tissue-location-specific transcription programs drive tumor dependencies in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lijing Yang,Lei Tu,Shilpa Bisht,Yiqing Mao,Daniel Petkovich,Sara-Jayne Thursby,Jinxiao Liang,Nibedita Patel,Ray-Whay Chiu Yen,Tina Largent,Cynthia Zahnow,Malcolm Brock,Kathy Gabrielson,Kevan J Salimian,Stephen B Baylin,Hariharan Easwaran

Journal of pineal research 74:e12846 PubMed36428267

2022

Continuous light exposure influences luteinization and luteal function of ovary in ICR mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Long Li,Hai-Jing Zhu,Qin Zhang,Yong-Shi Li,Yan-Chu Li,Xie Feng,Rui-Ying Yuan,Qian-Qian Sha,Jun-Yu Ma,Shi-Ming Luo,Qing-Yuan Sun,Lei-Ning Chen,Xiang-Hong Ou

Aging 14:8167-8178 PubMed36084952

2022

Downregulation of senescence-associated secretory phenotype by knockdown of secreted frizzled-related protein 4 contributes to the prevention of skin aging.

Applications

Unspecified application

Species

Unspecified reactive species

Kento Takaya,Toru Asou,Kazuo Kishi

Pharmaceutics 14: PubMed35893786

2022

In Vivo Evaluation of an Antibody-Functionalized Lipoidal Nanosystem for Schistosomiasis Intervention.

Applications

Unspecified application

Species

Unspecified reactive species

Tayo A Adekiya,Pradeep Kumar,Pierre P D Kondiah,Yahya E Choonara

Cell reports 39:110575 PubMed35417710

2022

Brown adipose tissue involution associated with progressive restriction in progenitor competence.

Applications

Unspecified application

Species

Unspecified reactive species

Zan Huang,Zengdi Zhang,Zahra Moazzami,Ryan Heck,Ping Hu,Hezkiel Nanda,Kaiqun Ren,Zequn Sun,Alessandro Bartolomucci,Yan Gao,Dongjun Chung,Weiyun Zhu,Steven Shen,Hai-Bin Ruan
View all publications
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