AB230425

Anti-SFRP5 antibody

Name: Anti-SFRP5 antibody (ab230425) | Abcam
Brand: Abcam Limited
SKU: AB230425

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(9 Publications)

Rabbit Polyclonal SFRP5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human SFRP5 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FRP1B, SARP3, SFRP5, Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, Secreted apoptosis-related protein 3, FRP-1b, SARP-3

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP5 antibody (AB230425)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP5 antibody (AB230425)

Formalin-fixed, paraffin-embedded human colon tissue stained for SFRP5 with ab230425 at 5 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SFRP5 antibody (AB230425)

All lanes:

Western blot - Anti-SFRP5 antibody (ab230425) at 1/1000 dilution

All lanes:

mouse heart tissue lysate at 35 µg

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SFRP5 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q5T4F7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRP5 also known as Secreted Frizzled-Related Protein 5 is a protein with a mass of approximately 36 kDa. It belongs to the family of secreted frizzled-related proteins that modulate Wnt signaling pathways. This protein acts as a modulator for Wnt ligands by binding to them and preventing their interaction with Frizzled receptors on the cell surface. SFRP5 is particularly expressed in adipose tissues and the retina where it affects processes related to these tissues.

Biological function summary

SFRP5 plays a significant role in cellular growth and differentiation. It acts as an antagonist to the Wnt signaling pathway and helps to maintain tissue homeostasis. By forming a complex with Wnt ligands it regulates the signaling processes important for development and cell proliferation. The inhibitory effect of SFRP5 on Wnt signaling has implications in controlling inflammation and metabolic functions specifically in adipose tissue.

Pathways

SFRP5 is involved in the canonical Wnt signaling pathway and the non-canonical Wnt/planar cell polarity pathway. These pathways are essential in embryonic development and maintaining adult tissue function. SFRP5 interacts with proteins such as beta-catenin in the canonical pathway and influences their downstream effects. These interactions help regulate cellular adhesion and migration which are important during developmental processes and in tissue repair mechanisms.

SFRP5 has been linked to metabolic conditions such as obesity and diabetes. In obesity its regulatory effects on inflammation and metabolism in adipose tissue become significant. SFRP5 works with adiponectin another protein involved in metabolic pathways to influence these conditions. In diabetes changes in SFRP5 expression can impact insulin sensitivity contributing to disease progression. The modulation of Wnt signaling by SFRP5 also has potential implications in tumorigenesis although this area requires further research to fully understand the interactions involved.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.

See full target information SFRP5

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:32 PubMed39773456

2025

PRAF2 as a novel biomarker for breast cancer with machine learning and experimentation validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Zilin Bi,Hongguang Bo,Junyi Xu,Rui Sha,Zhaocai Yin,Changsheng Yu,Yufa Xu,Xiaomeng Shi,Wenbo Song,Bin Chen,Yabing Wang,Qian Zhang,Jianping Chen

iScience 27:111517 PubMed39759008

2025

The mesenteric adipokine SFRP5 alleviated intestinal epithelial apoptosis improving barrier dysfunction in Crohn's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofeng Zhang,Lugen Zuo,Xue Song,Wenjing Zhang,Zi Yang,Zhiyuan Wang,Yibing Guo,Sitang Ge,Lian Wang,Yueyue Wang,Zhijun Geng,Jing Li,Jianguo Hu

Open medicine (Warsaw, Poland) 19:20240934 PubMed38584843

2024

Secreted frizzled-related protein 5 protects against renal fibrosis by inhibiting Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Deng,Dongli Tian,Yahui Wang,Yu Bai,Zongli Diao,Wenhu Liu

JCI insight 8: PubMed36927955

2023

Loss-of-function variant in SPIN4 causes an X-linked overgrowth syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Julian C Lui,Jacob Wagner,Elaine Zhou,Lijin Dong,Kevin M Barnes,Youn Hee Jee,Jeffrey Baron

Bioengineered 13:11656-11667 PubMed35506262

2022

Secreted frizzled-related protein 5 promotes angiogenesis of human umbilical vein endothelial cells and alleviates myocardial injury in diabetic mice with myocardial infarction by inhibiting Wnt5a/JNK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nian Ding,Chenghong Zheng

Molecular medicine reports 24: PubMed34558644

2021

PPARγ attenuates hepatic inflammation and oxidative stress of non‑alcoholic steatohepatitis via modulating the miR‑21‑5p/SFRP5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiying Zhang,Fang Deng,Yuping Zhang,Xiaohong Zhang,Jianfei Chen,Youzhao Jiang

Kidney & blood pressure research 46:758-767 PubMed34469882

2021

Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Deng,Xue Han,Zongli Diao,Wenhu Liu

Experimental and molecular pathology 118:104591 PubMed33285209

2020

The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohui Shi,Min Xu,Xiaodong Chen,Jian Wang,Tianfeng Zhao,Dingjun Zha

Cell death & disease 11:378 PubMed32424151

2020

BCL-xL/BCL2L1 is a critical anti-apoptotic protein that promotes the survival of differentiating pancreatic cells from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Larry Sai Weng Loo,Andreas Alvin Purnomo Soetedjo,Hwee Hui Lau,Natasha Hui Jin Ng,Soumita Ghosh,Linh Nguyen,Vidhya Gomathi Krishnan,Hyungwon Choi,Xavier Roca,Shawn Hoon,Adrian Kee Keong Teo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SFRP5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRP5 antibody (AB230425)

Western blot - Anti-SFRP5 antibody (AB230425)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise