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AB254733

Anti-SFRS11 antibody

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(2 Publications)

Rabbit Polyclonal SFRS11 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SRSF11 aa 100-300.

View Alternative Names

SFRS11, SRSF11, Serine/arginine-rich splicing factor 11, Arginine-rich 54 kDa nuclear protein, p54

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)

Paraffin-embedded human lymph node tissue stained for SFRS11 using ab254733 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)

Paraffin-embedded human prostate tissue stained for SFRS11 using ab254733 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-SFRS11 antibody (AB254733)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SFRS11 antibody (AB254733)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling SFRS11 using ab254733 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)

Paraffin-embedded human pancreas tissue stained for SFRS11 using ab254733 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS11 antibody (AB254733)

Paraffin-embedded human endometrium tissue stained for SFRS11 using ab254733 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-SFRS11 antibody (AB254733)
  • WB

Supplier Data

Western blot - Anti-SFRS11 antibody (AB254733)

All lanes:

Western blot - Anti-SFRS11 antibody (ab254733) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

SFRS11 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SRSF11 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

Q05519

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SFRS11 protein also known as splicing factor arginine/serine-rich 11 functions mechanically as a splicing factor involved in the regulation of pre-mRNA processing. It has a molecular mass of approximately 54 kDa. SFRS11 expresses in various tissues with higher levels in the brain and testis. This protein belongs to the serine/arginine-rich (SR) family and contains RNA recognition motifs that facilitate its interaction with target RNA sequences.
Biological function summary

This splicing factor plays an essential role in the regulation of gene expression by modulating alternative splicing events. SFRS11 can influence the inclusion or exclusion of specific RNA sequences during mRNA formation. It may work as part of a spliceosomal complex where its role in the splicing process is critical for generating diverse mRNA isoforms. This regulatory capacity impacts diverse cellular processes like cell differentiation and apoptosis.

Pathways

Splicing factors such as SFRS11 are integral to both the splicing pathway and the wider RNA processing network. Within these pathways SFRS11 interacts with other splicing proteins like SF3B1 and SRSF2 which coordinate to ensure accurate and efficient spliceosome assembly and function. These interactions contribute to the regulation of genetic expression patterns in response to cellular signals and stress conditions.

Aberrant SFRS11 function associates with certain cancers and neurodegenerative diseases. Improper alternative splicing due to SFRS11 mutations or misregulation may lead to the production of oncogenic mRNA isoforms linking it to cancer progression. Related proteins like SRPK1 a kinase regulating SR proteins including SFRS11 are often implicated in these pathologies highlighting the significance of this splicing factor in maintaining normal cellular function and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May function in pre-mRNA splicing.
See full target information SRSF11
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:4535-4547 PubMed37750019

2023

Tetraspanin 1 regulates papillary thyroid tumor growth and metastasis through c-Myc-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihua Han,Changming Xie,Bo Liu,Yan Wang,Rui Pang,Wen Bi,Rinan Sheng,Guoqing He,Lingyu Kong,Jiawei Yu,Zhaoming Ding,Lili Chen,Jinliang Jia,Jiewu Zhang,Chunlei Nie

Clinical and translational medicine 12:e1113 PubMed36394206

2022

Alternative splicing of HSPA12A pre-RNA by SRSF11 contributes to metastasis potential of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Jie Pan,Fu-Chun Huo,Meng-Jie Kang,Bo-Wen Liu,Meng-Di Wu,Dong-Sheng Pei
View all publications
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Product promise

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