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AB73893

Anti-SFRS4/SRSF4 antibody

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(3 Publications)

Rabbit Polyclonal SFRS4/SRSF4 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF4.

View Alternative Names

SFRS4, SRP75, SRSF4, Serine/arginine-rich splicing factor 4, Pre-mRNA-splicing factor SRP75, SRP001LB

1 Images
Western blot - Anti-SFRS4/SRSF4 antibody (AB73893)
  • WB

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Western blot - Anti-SFRS4/SRSF4 antibody (AB73893)

All lanes:

Western blot - Anti-SFRS4/SRSF4 antibody (ab73893) at 1/500 dilution

Lane 1:

extracts from LoVo cells at 20 µg

Lane 2:

extracts from LoVo cells at 20 µg with immunizing peptide

Predicted band size: 57 kDa

Observed band size: 20 kDa,48 kDa,57 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SRSF4. The exact immunogen used to generate this antibody is proprietary information.

Q08170

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRS4 also known as SRSF4 or splicing factor SRp75 is a serine/arginine-rich splicing factor that plays a central role in pre-mRNA splicing. It has a molecular mass of approximately 75 kDa. SRSF4 contributes to the accurate assembly of spliceosomes by binding to specific RNA sequences through its RNA recognition motifs. This protein is expressed ubiquitously with higher expression levels observed in tissues like the brain and heart indicating its importance for proper cellular functions in these areas.
Biological function summary

Splicing is an important process in the regulation of gene expression and SRSF4 actively participates in this essential function by guiding the splicing machinery to the appropriate splice sites. SRSF4 is part of the highly conserved SR protein family which functions collectively as a complex to regulate alternative splicing events. Alternative splicing allows for the generation of multiple protein isoforms from a single gene significantly expanding the proteomic diversity.

Pathways

Alternative splicing regulated by SRSF4 integrates into the larger RNA processing and post-transcriptional modification network. SRSF4 is involved in the mRNA splicing pathway and interacts with various other splicing factors such as SRSF1 and U2AF. These interactions allow precise execution of splicing and efficient production of mature messenger RNA preparatory for translation processes downstream.

Mutations or dysregulation of SRSF4 have connections with several conditions including cancer and neurodegenerative diseases. Abnormal splicing patterns regulated by SRSF4 can lead to the production of oncogenic isoforms in various cancers. In neurodegenerative diseases its altered function might disturb neuronal alternative splicing patterns impacting protein diversity vital for neuronal functions. SRSF4 interacts with proteins like SRSF2 and hnRNPs in these pathological contexts influencing the disease progression through defective splicing mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10.
See full target information SRSF4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:14503 PubMed29109428

2017

Serial-omics of P53-/-, Brca1-/- Mouse Breast Tumor and Normal Mammary Gland.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne B Breitkopf,Mateus De Oliveira Taveira,Min Yuan,Gerburg M Wulf,John M Asara

Molecular reproduction and development 82:976-85 PubMed26308373

2015

Androgen receptor binding to an androgen-responsive element in the promoter of the Srsf4 gene inhibits its expression in mouse Sertoli cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Guo,Yuchi Li,Manling Luo,Shouren Lin,Jianbo Chen,Qian Ma,Yanli Gu,Zhimao Jiang,Yaoting Gui

PloS one 7:e51497 PubMed23284704

2012

Regulation of Mcl-1 by SRSF1 and SRSF5 in cancer cells.

Applications

WB

Species

Human

Hannah L Gautrey,Alison J Tyson-Capper
View all publications
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