AB137247

Anti-SFRS7/SRSF7 antibody

Name: Anti-SFRS7/SRSF7 antibody (ab137247) | Abcam
Brand: Abcam Limited
SKU: AB137247
Price: 520 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal SFRS7/SRSF7 antibody. Suitable for IHC-P, IP and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SRSF7 aa 50-150.

View Alternative Names

SFRS7, SRSF7, Serine/arginine-rich splicing factor 7, Splicing factor 9G8

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody (AB137247)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody (AB137247)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling SRSF7/SRSF7 with ab137247 at 1/5000 (0.2μg/ml) and 1/1000 (1μg/ml) . Detection : DAB.

Unknown

Immunoprecipitation - Anti-SFRS7/SRSF7 antibody (AB137247)

Immunoprecipitation with ab137247 at 6μg/mg lysate. 1 mg of HeLa whole cell lysate used for IP (20% of IP loaded). Subsequent WB detection used an anti-SFRS7/SRSF7 antibody which recognizes a downstream epitope at 1 μg/ml. Detected by chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-SFRS7/SRSF7 antibody (ab137247)

Predicted band size: 27 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human SRSF7 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

Q16629

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab137247 was affinity purified using an epitope specific to SRSF7/SRSF7 immobilized on solid support

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRS7 also known as SRSF7 is a member of the serine/arginine-rich family of proteins that functions as an essential splicing factor. It has a mass of approximately 27 kDa. SRSF7 shows expression in various tissues with notable presence in the nucleoplasm of cells. Mechanically SRSF7 has binding affinity for RNA sequences facilitating alternative splicing by interacting with pre-mRNA. This protein plays a significant role in post-transcriptional regulation influencing the maturation and diversity of mRNA transcripts.

Biological function summary

SRSF7 contributes to the regulation of gene expression and is involved in spliceosome formation though it is not strictly part of the spliceosomal core complex. It influences exon skipping and inclusion which affects various functional mRNA variants. SRSF7 enables cellular responses to changes in internal and external environments by altering gene expression patterns. This flexibility positions it as an important player in processes such as cell cycle regulation and apoptosis.

Pathways

SRSF7 operates within the general mRNA processing pathway and plays a part in the spliceosome pathway. SRSF7 interacts with other splicing factors like SRSF1 which promotes its functional diversity. These proteins collectively enable precise and correct splicing of mRNA which is critical in maintaining cellular homeostasis and responding to physiological changes.

Abnormalities in SRSF7 expression or function can relate to cancer and neurodegenerative disorders. Dysregulation of mRNA splicing where SRSF7 plays a role can drive oncogenic transformations in cells linking it to various forms of cancer. Also SRSF7 and splicing-related proteins such as hnRNPs are observed in dysfunctional states within neurodegenerative conditions suggesting splicing alterations contribute to disease pathogenesis. Understanding SRSF7's role allows for better comprehension of these disorders and potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.

See full target information SRSF7

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40649911

2025

Multi-Omics Integration: Predicting Progression and Optimizing Clinical Treatment of Hepatocellular Carcinoma Through Malignant-Cell-Related Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Qianwen Wang,Lingli Cheng,Honglin Yan,Jingping Yuan

Heliyon 10:e31972 PubMed38868058

2024

Downregulation of hsa_circTLK1 represses non-small cell lung cancer progression by regulating miR-876-3p/SRSF7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinzhe Dong,Hui Tian,Peng Ren,Yanxia Liu,Lin Wang

Biochimica et biophysica acta. Molecular basis of disease 1870:166983 PubMed38070581

2023

BRD9 status is a major contributor for cysteine metabolic remodeling through MST and EAAT3 modulation in malignant melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Hipólito,Renato Xavier,Cheila Brito,Ana Tomás,Isabel Lemos,Luís C Cabaço,Fernanda Silva,Abel Oliva,Duarte C Barral,João B Vicente,Luís G Gonçalves,Marta Pojo,Jacinta Serpa

Viruses 14: PubMed35062264

2021

The Thiazole-5-Carboxamide GPS491 Inhibits HIV-1, Adenovirus, and Coronavirus Replication by Altering RNA Processing/Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Subha Dahal,Ran Cheng,Peter K Cheung,Terek Been,Ramy Malty,Melissa Geng,Sarah Manianis,Lulzim Shkreta,Shahrazad Jahanshahi,Johanne Toutant,Rose Chan,Sean Park,Mark A Brockman,Mohan Babu,Samira Mubareka,Karen Mossman,Arinjay Banerjee,Scott Gray-Owen,Martha Brown,Walid A Houry,Benoit Chabot,David Grierson,Alan Cochrane

PLoS pathogens 16:e1008307 PubMed32069328

2020

An activator of G protein-coupled receptor and MEK1/2-ERK1/2 signaling inhibits HIV-1 replication by altering viral RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Raymond W Wong,Ahalya Balachandran,Peter K Cheung,Ran Cheng,Qun Pan,Peter Stoilov,P Richard Harrigan,Benjamin J Blencowe,Donald R Branch,Alan Cochrane

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SFRS7/SRSF7 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody (AB137247)

Immunoprecipitation - Anti-SFRS7/SRSF7 antibody (AB137247)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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