Skip to main content

Rabbit Recombinant Monoclonal SFRS7/SRSF7 antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Recombinant fragment - Human, Human, Mouse samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (AB322406), expandable thumbnail
  • Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (AB322406), expandable thumbnail
  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (AB322406), expandable thumbnail
  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (AB322406), expandable thumbnail
  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (AB322406), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PIP
Human
Tested
Tested
Not recommended
Tested
Mouse
Tested
Tested
Tested
Tested
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Recombinant fragment - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Species

Mouse

Dilution info

1/30

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Required for pre-mRNA splicing. Can also modulate alternative splicing in vitro. Represses the splicing of MAPT/Tau exon 10. May function as export adapter involved in mRNA nuclear export such as of histone H2A. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. RNA-binding is semi-sequence specific.

Alternative names

Rabbit Recombinant Monoclonal SFRS7/SRSF7 antibody. Suitable for WB, ICC/IF, IHC-P, IP and reacts with Recombinant fragment - Human, Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR28621-501

Purification technique

Affinity purification Protein A

Specificity

Unsuitable for human IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    SFRS7/SRSF7 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab322406 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322406 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lane 2: ab322406 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322406 in Neuro-2a whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

    All lanes: Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406) at 1/30 dilution

    All lanes: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    SFRS7/SRSF7 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab322406 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322406 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 2: ab322406 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322406 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

    All lanes: Immunoprecipitation - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 3s

  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human SRSF3.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406) at 1/1000 dilution

    Lane 1: No-tag human SFRS7/SRSF7 fragment at 10 ng

    Lane 2: His-tagged human SRSF3 fragment at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa, 20 kDa

    Exposure time: 26s

  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406) at 1/1000 dilution

    All lanes: Mouse testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa, 124 kDa

    Exposure time: 180s

  • Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HL-60, EL4.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 5: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 30 kDa, 36 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling SFRS7/SRSF7 with ab322406 at 1/1000 (0.53 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing nuclear staining in Neuro-2a cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: EL4.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling SFRS7/SRSF7 with ab322406 at 1/1000 (0.53 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing nuclear staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: HL-60.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Immunohistochemical analysis of paraffin-embedded (A) Neuro-2a (mouse neuroblastoma neuroblast) cell pellet; (B) EL4 (mouse lymphoma T lymphocyte) cell pellet (low expression) tissue labeling SFRS7/SRSF7 with ab322406 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on (A) Neuro-2a cell pellet and weak staining on (B) EL4 cell pellet.

    The section was incubated with ab322406 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling SFRS7/SRSF7 with ab322406 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse testis.

    The section was incubated with ab322406 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS7/SRSF7 antibody [EPR28621-501] (ab322406)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SFRS7/SRSF7 with ab322406 at 1/500 (1.06 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with ab322406 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com