Rabbit Polyclonal SFRS8 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SFSWAP.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SFSWAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q12872
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Gorilla | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-5 µg | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rhesus monkey, Gorilla, Orangutan | Dilution info - | Notes - |
Target data
Function
Plays a role as an alternative splicing regulator. Regulate its own expression at the level of RNA processing. Also regulates the splicing of fibronectin and CD45 genes. May act, at least in part, by interaction with other R/S-containing splicing factors. Represses the splicing of MAPT/Tau exon 10.
Alternative names
SFRS8, SWAP, SFSWAP, Suppressor of white apricot protein homolog
Recommended products
Rabbit Polyclonal SFRS8 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SFSWAP.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SFSWAP. The exact immunogen used to generate this antibody is proprietary information. Database link Q12872
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab72044 was affinity purified using an epitope specific to SFRS8 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SFRS8 also known as SR protein splicing factor 8 or SFRS8 is a serine/arginine-rich splicing factor involved in the regulation of alternative splicing in pre-mRNA. The protein is approximately 81 kDa and is expressed in various tissues including the brain heart and skeletal muscle. SFRS8 functions as a splicing regulator modulating the assembly of spliceosomes through its interaction with other splicing factors and components of the messenger ribonucleoprotein complexes.
- Biological function summary
SFRS8 plays key roles in the regulation of alternative splicing influencing the diversity of protein isoforms that cells can produce. It is often part of larger spliceosome complexes functioning with other serine/arginine-rich proteins to ensure accurate and efficient splicing of introns from pre-mRNAs. This regulatory capability is important for maintaining gene expression and cellular functions particularly in tissues with preferential expression like the nervous and muscular systems.
- Pathways
SFRS8 integrates within the spliceosomal and mRNA processing pathways. It coordinates actions with other serine/arginine-rich splicing factors like SRSF1 and SRSF9 which participate in the splicing enhancer complexes. These pathways are essential in post-transcriptional regulation where SFRS8 helps ensure proper exon inclusion or skipping. This regulation is especially significant in pathways leading to protein diversification and cellular differentiation processes.
- Associated diseases and disorders
SFRS8 impacts several disorders including cancer and spinal muscular atrophy. Aberrations in SFRS8 expression or function can lead to defective splicing and subsequent production of abnormal protein isoforms. In cancer altered SFRS8 activity can affect cell proliferation and survival through interactions with other factors like hnRNP proteins. Similarly in spinal muscular atrophy improper splicing can disrupt the function of SMN proteins closely tied to motor neuron degeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS8 antibody (ab72044)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SFRS8 with ab72044 at 1/200 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-SFRS8 antibody (ab72044)
Detection of SFRS8 by Western Blot of Immunprecipitate.
ab72044 at 1µg/ml staining SFRS8 in HeLa whole cell lysate immunoprecipitated using ab72044 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.All lanes: Immunoprecipitation - Anti-SFRS8 antibody (ab72044)
Predicted band size: 105 kDa
Western blot - Anti-SFRS8 antibody (ab72044)
All lanes: Western blot - Anti-SFRS8 antibody (ab72044) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 5 µg
Predicted band size: 105 kDa
Observed band size: 150 kDa
Exposure time: 3min
Downloads
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