AB236414

Anti-SFRS9/SRSF9 antibody [OTI5G7]

Name: Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414) | Abcam
Brand: Abcam Limited
SKU: AB236414
Price: 525 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal SFRS9/SRSF9 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SRSF9.

View Alternative Names

SFRS9, SRP30C, SRSF9, Serine/arginine-rich splicing factor 9, Pre-mRNA-splicing factor SRp30C

12 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human lung carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human breast adenocarcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human pancreas carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human kidney carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human lymph node tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human pancreas tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human thyroid carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human prostate tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human colon adenocarcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human kidney tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Paraffin-embedded human prostate carcinoma tissue stained for SFRS9/SRSF9 using ab236414 at 1/150 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Lanes 1, 2 and 4:

Western blot - Anti-SFRS9/SRSF9 antibody [OTI5G7] (ab236414) at 1/500 dilution

Lane 3:

Anti-HSP90AB1

Lane 1:

Wild-type HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lanes 2 and 4:

SFRS9/SRSF9 knockout HEK-293T whole cell lysate at 10 µg

Lane 3:

Wild-type HEK-293T whole cell lysate at 10 µg

Predicted band size: 26 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI5G7

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SRSF9.

Q13242

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Perform heat-induced epitope retrieval by Tris-EDTA, pH 8.0.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Clone OTI5G7 (formerly 5G7).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Purified from cell culture supernatant.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFRS9 also known as SRSF9 or SRp30c is a serine/arginine-rich splicing factor critical for splicing regulation in pre-mRNA processing. Its molecular mass is approximately 28 kDa. SFRS9 is widely expressed in various tissues including the brain liver and heart. This protein belongs to the SR protein family characterized by their role in spliceosome assembly and exon selection during RNA splicing.

Biological function summary

SFRS9 contributes to the regulation of alternative splicing which increases the diversity of the proteome. It does not work alone but within the spliceosome complex where it interacts with other splicing factors. This activity helps determine which exons are included or excluded in the final mRNA affecting protein variety and cellular function. By controlling splicing events SFRS9 can influence gene expression and cellular responses to environmental cues.

Pathways

SFRS9 serves as a significant player in the RNA processing pathway. It helps modulate gene expression post-transcriptionally by governing the splicing fate of pre-mRNA transcripts. Its role intersects with pathways like the mRNA surveillance pathway where it ensures correct mRNA synthesis and processing. SFRS9's activity is also associated with other splicing factors such as SF2/ASF in regulating alternative splicing.

SFRS9 has connections to cancer and neurodegenerative disorders. Aberrations in alternative splicing patterns regulated by SFRS9 may lead to oncogenic transformations and neuronal dysfunction. In cancer its altered splicing activity can result in tumor progression. Interactions between SFRS9 and proteins involved in splicing like hnRNPs may have contributions in abnormal splicing patterns linked with these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10.

See full target information SRSF9

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer biology & therapy 25:2427415 PubMed39530604

2024

A positive feedback loop of SRSF9/USP22/ZEB1 promotes the progression of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Ming Hu,Jie Min,Xin Li

Animal bioscience 38:303-315 PubMed39210798

2024

shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shu Zhen Jiang,Li Bo Huang,Wei Ren Yang

NAR cancer 4:zcab052 PubMed35047826

2022

Comprehensive profiling of mRNA splicing indicates that GC content signals altered cassette exon inclusion in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett T Graham,Saravana P Selvanathan,Stefan K Zöllner,Emily Stahl,Adam Shlien,Natasha J Caplen,Aykut Üren,Jeffrey A Toretsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SFRS9/SRSF9 antibody [OTI5G7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Western blot - Anti-SFRS9/SRSF9 antibody [OTI5G7] (AB236414)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise