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AB240192

Anti-SFT antibody [EPR13000(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SFT antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

SFT, UBC5A, UBCH5, UBCH5A, UBE2D1, Ubiquitin-conjugating enzyme E2 D1, (E3-independent) E2 ubiquitin-conjugating enzyme D1, E2 ubiquitin-conjugating enzyme D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human prostate tissue labeling SFT with ab176561 at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)

Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling SFT (red) with ab176561 at a 1/10 dilution, or negative control rabbit IgG (green)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human thyroid gland carcinoma tissue labeling SFT with ab176561 at a 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)
  • IP

Supplier Data

Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] - BSA and Azide free (AB240192)

Western blot analysis on immunoprecipitation pellet from Human skeletal muscle lysate labeling SFT with ab176561 at 1/10 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176561).

All lanes:

Immunoprecipitation - Anti-SFT antibody [EPR13000(B)] (<a href='/en-us/products/primary-antibodies/sft-antibody-epr13000b-ab176561'>ab176561</a>)

Predicted band size: 17 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13000(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IP, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab240192 is the carrier-free version of ab176561.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein SFT also known as surfactant protein or SP plays a role in reducing surface tension in the lungs. It's a glycoprotein with a known mass of around 32 kDa. SFT mainly gets expressed in the alveolar type II cells in the lungs where it's secreted into the lung's extracellular lining. Its primary function involves aiding in lung stability and preventing alveolar collapse during respiration.
Biological function summary

Surfactant proteins contribute to innate immune defense and lung homeostasis. SFT is a component of the surfactant complex which includes other proteins and lipids. This protein acts at the air-liquid interface in the alveoli and engages in host defense functions. SFT activates alveolar macrophages and binds to a range of pathogens enhancing their clearance from the respiratory tract. It helps regulate inflammatory responses and maintain the balance of surfactant components in the lungs.

Pathways

SFT plays an integral role in the pulmonary surfactant metabolism pathway and the immune response pathway. In the pulmonary surfactant metabolism pathway it interacts with proteins like ABCA3 and lipids like phosphatidylcholine to ensure proper surfactant composition and recycling. In the immune response pathway SFT partners with complement proteins and defensins supporting pathogen clearance and preventing lung infections.

SFT relates primarily to respiratory distress syndrome (RDS) and pulmonary alveolar proteinosis (PAP). In RDS an insufficient amount or dysfunction of surfactant proteins including SFT leads to alveolar collapse and impaired gas exchange. In PAP the protein's accumulation affects surfactant homeostasis disrupting lung function. These diseases tie SFT to other proteins like GM-CSF and ABCA3 which influence surfactant production and regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins (PubMed : 22496338). In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed : 20061386). Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1 (PubMed : 18042044, PubMed : 18359941). Ubiquitinates STUB1-associated HSP90AB1 in vitro (PubMed : 18042044). Lacks inherent specificity for any particular lysine residue of ubiquitin (PubMed : 18042044). Essential for viral activation of IRF3 (PubMed : 19854139). Mediates polyubiquitination of CYP3A4 (PubMed : 19103148).
See full target information UBE2D1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 204:3400-3415 PubMed32358019

2020

Suppression of Mll1-Complex by Stat3/Cebpβ-Induced miR-21a/21b/181b Maintains the Accumulation, Homeostasis, and Immunosuppressive Function of Polymorphonuclear Myeloid-Derived Suppressor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqian Zhang,Xu Huang,Enlin Wang,Yugang Huang,Rongcun Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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