Rabbit Polyclonal SFT2D2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SFT2D2 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SFT2D2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95562
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex.
Alternative names
UNQ512/PRO1027, SFT2D2, Vesicle transport protein SFT2B, SFT2 domain-containing protein 2
Recommended products
Rabbit Polyclonal SFT2D2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SFT2D2 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SFT2D2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O95562
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SFT2D2 also known as Suppressor of Fused (Sof1) in yeast is a protein involved in membrane trafficking and sorting within cells. It has a mass of approximately 21 kDa and is localized in the membranes of the Golgi apparatus and cytoplasmic vesicles. Expression of SFT2D2 occurs in various tissues with high levels observed in the liver and kidney. The protein possesses transmembrane domains that allow it to facilitate movement of vesicles between organelles.
- Biological function summary
SFT2D2 plays a role in the Golgi apparatus' function and vesicular transport processes. It is part of a larger protein complex that helps mediate vesicle fusion and cargo transport. This protein ensures accurate sorting and delivery of proteins and lipids which are essential for maintaining cellular homeostasis. It is especially vital in regulating the processing and maturation of proteins that transit through the secretory pathway.
- Pathways
SFT2D2 functions in the intracellular trafficking and secretory pathways. It interacts with the SNARE protein family to perform its role in vesicle docking and fusion. Additionally SFT2D2 has relevance in the secretory pathway where it contributes to the optimal functioning of the endoplasmic reticulum (ER) to Golgi transport. This coordination with proteins such as Syntaxin 5 ensures seamless progression of synthesized proteins to their destinations.
- Associated diseases and disorders
Alterations in SFT2D2 expression or structure might relate to metabolic dysfunctions including liver diseases. Disruption to SFT2D2 pathways also indicates a potential link to neurodegenerative disorders due to impaired vesicular trafficking. Relatedly improper association with SNARE proteins can exacerbate these conditions highlighting the importance of SFT2D2 in disease pathogenesis. Understanding its role could aid in discovering therapeutic targets in related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SFT2D2 antibody (ab236899)
All lanes: Western blot - Anti-SFT2D2 antibody (ab236899) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse kidney tissue lysate
Lane 3: Mouse thymus tissue lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SFT2D2 antibody (ab236899)
MCF7 (Human breast adenocarcinoma cell line) cells stained for SFT2D2 (green) using ab236899 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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