AB190087

Anti-SFTPA1 + SFTPA2 antibody

Name: Anti-SFTPA1 + SFTPA2 antibody (ab190087) | Abcam
Brand: Abcam Limited
SKU: AB190087
Price: 630 USD
Availability: InStock

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(3 Publications)

Goat Polyclonal Pulmonary surfactant-associated protein A1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human SFTPA1 aa 100-150.

View Alternative Names

COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA1, Pulmonary surfactant-associated protein A1, PSP-A, PSPA, SP-A, SP-A1, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein, Collectin-4

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody (AB190087)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody (AB190087)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human lung tissue labeling SFTPA1 + SFTPA2 with ab190087 at 5 μg/ml.

Supplier Data

Western blot - Anti-SFTPA1 + SFTPA2 antibody (AB190087)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-SFTPA1 + SFTPA2 antibody (ab190087) at 0.01 µg/mL

All lanes:

Human lung tissue lysate in RIPA buffer at 35 µg

Predicted band size: 26 kDa

Observed band size: 30 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SFTPA1 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q8IWL2

Specificity

ab190087 is expected to recognize all the reported isoforms of A1 (NP_005402.3; NP_001087239.2; NP_001158117.1; NP_001158118.1) and A2 ( NP_001092138.1), Reported variants represent identical protein: NP_001158116.1, NP_005402.3, NP_001158119.1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SFTPA1 and SFTPA2 also known as Surfactant Protein A1 and Surfactant Protein A2 are important components of the pulmonary surfactant system. These proteins have masses of approximately 26 kDa each. They are expressed in alveolar type II cells in the lungs and are secreted into the alveolar space. The primary function of SFTPA1 and SFTPA2 is to reduce surface tension in the alveoli preventing collapse during exhalation and supporting efficient gas exchange.

Biological function summary

Surfactant Proteins A1 and A2 play essential roles in the immune defense of the lungs. They operate as part of the surfactant protein complex which includes other proteins like SP-B SP-C and SP-D. They bind to various pathogens including bacteria and viruses promoting their clearance by enhancing phagocytosis by alveolar macrophages. This immune function is vital for maintaining pulmonary health and protecting against infections.

Pathways

These proteins function within the innate immune response and inflammation pathways in the lung. In these pathways SFTPA1 and SFTPA2 interact with toll-like receptors (TLRs) and CD14 binding to pattern recognition receptors (PRRs) to initiate defense reactions against pathogens. Their action complements other immune proteins like Mannose-Binding Lectin (MBL) and is linked with receptor-mediated pathways that modulate inflammation and immunity in the respiratory system.

Improperly functioning Surfactant Proteins A can lead to respiratory distress syndromes such as neonatal respiratory distress syndrome and acute respiratory distress syndrome. Mutations or deficiencies in these proteins have association with interstitial lung disease. Changes or dysregulation of SFTPA1 and SFTPA2 levels can alter lung immunity creating a link with inflammatory responses and diseases influenced by SP-D and related surfactant proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (By similarity).. (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule (PubMed : 17158455, PubMed : 21203928). Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages (PubMed : 21123169).. (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen (PubMed : 15845487, PubMed : 25139904). When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen (PubMed : 25139904).

See full target information SFTPA1

Additional targets

SFTPA2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 16:1658389 PubMed40964620

2025

Blood-spinal cord barrier alterations in a mouse model of centrifugation-induced hypergravity.

Applications

Unspecified application

Species

Unspecified reactive species

David Dubayle,Jean-Luc Morel,Sighild Lemarchant

Frontiers in genetics 15:1464471 PubMed39935693

2025

Machine learning potential predictor of idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chenchun Ding,Quan Liao,Renjie Zuo,Shichao Zhang,Zhenzhen Guo,Junjie He,Ziwei Ye,Weibin Chen,Sunkui Ke

American journal of physiology. Lung cellular and molecular physiology 326:L524-L538 PubMed38375572

2024

Multivalent, calcium-independent binding of surfactant protein A and D to sulfated glycosaminoglycans of the alveolar epithelial glycocalyx.

Applications

Unspecified application

Species

Unspecified reactive species

Rabia Avcibas,Anna Vermul,Vladimir Gluhovic,Nico Boback,Raquel Arroyo,Paul Kingma,Miriam Isasi-Campillo,Lucia Garcia-Ortega,Matthias Griese,Wolfgang M Kuebler,Matthias Ochs,Daniel Lauster,Elena Lopez-Rodriguez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SFTPA1 + SFTPA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody (AB190087)

Western blot - Anti-SFTPA1 + SFTPA2 antibody (AB190087)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (3)

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