Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human lung tissue staining SFTPA1 + SFTPA2 with ab320648 at a 1/1000 dilution, ab213363 anti-CD68 used at 1/500 dilution and ab307666 anti-Uteroglobin used at a 1/2000 dilution.

Panel A : merged staining of anti-SFTPA1+SFTPA2 (magenta; Opal^™690), anti-CD68 (green; Opal^™520) and anti-Uteroglobin (gray; Opal^™570) on human lung.
Panel B : anti-SFTPA1+SFTPA2 staining alveolar type II cells in human lung.
Panel C : ant-CD68 staining macrophages in human lung.
Panel D : ant-Uteroglobin staining club cells in human lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab320648, ab211363 and ab307666 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins..

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human spleen. The section was incubated with ab320648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human kidney. The section was incubated with ab320648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human lung squamous cell carcinoma. The section was incubated with ab320648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in Type II pneumonocyte of human lung (PMID : 32049827). The section was incubated with ab320648 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. This antibody cross-reacts with overexpressed human SFTPA1 and SFTPA2. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution. In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human SFTPA1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human SFTPA2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : cerebellum, spleen, liver (PMID : 34181042). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 28 kDa,36 kDa

false

Exposure time: 180s