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Rabbit Recombinant Monoclonal Pulmonary surfactant-associated protein A1 antibody. Suitable for WB, IHC-P, mIHC and reacts with Transfected cell lysate - Human, Human samples.

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Images

Multiplex immunohistochemistry - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648), expandable thumbnail
  • Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (AB320648), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PmIHC
Human
Tested
Tested
Tested
Transfected cell lysate - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Target data

Function

In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages (By similarity). (Microbial infection) Recognition of M.tuberculosis by dendritic cells may occur partially via this molecule (PubMed:17158455, PubMed:21203928). Can recognize, bind, and opsonize pathogens to enhance their elimination by alveolar macrophages (PubMed:21123169). (Microbial infection) Binds M.pneumoniae CARDS toxin, serves as one receptor for this pathogen (PubMed:15845487, PubMed:25139904). When SFTPA1 is down-regulated by siRNA, less toxin binds to human cells and less vacuolization (a symptom of M.pneumoniae infection) is seen (PubMed:25139904).

Additional Targets

SFTPA2

Alternative names

COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA1, Pulmonary surfactant-associated protein A1, PSP-A, PSPA, SP-A, SP-A1, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein, Collectin-4

Recommended products

Rabbit Recombinant Monoclonal Pulmonary surfactant-associated protein A1 antibody. Suitable for WB, IHC-P, mIHC and reacts with Transfected cell lysate - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29098-6
Purification technique
Affinity purification Protein A
Specificity

The immunogen shares no sequence similarity with SFTPB, SFTPC or SFTPD. Based on the immunogen sequence, we do not expect this antibody to cross-react with SFTPB, SFTPC or SFTPD.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SFTPA1 and SFTPA2 also known as surfactant protein A1 and A2 are essential components of the pulmonary surfactant system. SFTPA1 and SFTPA2 are glycoproteins with a molecular mass of approximately 26 kDa. These proteins are mainly expressed in the lung alveolar type II epithelial cells. They play a critical role in reducing surface tension in the alveoli preventing lung collapse during respiration. SFTPA1 and SFTPA2 bind to lipids within the surfactant helping to maintain its structure and function.

Biological function summary

SFTPA1 and SFTPA2 are involved in the immune defense mechanisms of the lungs. Both proteins are part of the collectin family and form higher-order oligomeric structures. As a component of the surfactant protein complex they enhance phagocytosis of pathogens by alveolar macrophages and neutralize pathogens directly. Additionally they modulate inflammatory responses in the lung therefore contributing to the protection of pulmonary tissues.

Pathways

SFTPA1 and SFTPA2 are integral to the innate immune response and lipid metabolism pathways in the lungs. They interact with toll-like receptors (TLRs) and other surfactant proteins like surfactant protein D (SFTPD) to activate signaling pathways that lead to inflammation control. SFTPA1 and SFTPA2 also influence surfactant homeostasis within the lung environment contributing to the maintenance of respiratory function.

Associated diseases and disorders

SFTPA1 and SFTPA2 relate closely to respiratory distress syndrome (RDS) and pulmonary alveolar proteinosis (PAP). Mutations in SFTPA1 and SFTPA2 genes can impair surfactant function which often results in RDS particularly in premature infants. Moreover alterations in these proteins can also contribute to PAP where abnormal surfactant accumulation occurs. Their interplay with proteins like surfactant protein B (SFTPB) further links them to these pulmonary disorders as these proteins work together to ensure proper surfactant functionality.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Multiplex immunohistochemistry - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Multiplex immunohistochemistry - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human lung tissue staining SFTPA1 + SFTPA2 with ab320648 at a 1/1000 dilution, Anti-CD68 antibody [EPR20545] ab213363 anti-CD68 used at 1/500 dilution and Anti-Uteroglobin antibody [EPR27144-86] ab307666 anti-Uteroglobin used at a 1/2000 dilution.

    Panel A: merged staining of anti-SFTPA1+SFTPA2 (magenta; Opal™690), anti-CD68 (green; Opal™520) and anti-Uteroglobin (gray; Opal™570) on human lung.

    Panel B: anti-SFTPA1+SFTPA2 staining alveolar type II cells in human lung.

    Panel C: ant-CD68 staining macrophages in human lung.

    Panel D: ant-Uteroglobin staining club cells in human lung.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab320648, ab211363 and Anti-Uteroglobin antibody [EPR27144-86] ab307666 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins..

  • Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody cross-reacts with overexpressed human SFTPA1 and SFTPA2.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 2: 293T cells transfected with a human SFTPA1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Lane 3: 293T cells transfected with a human SFTPA2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 35 kDa, 36 kDa

    Exposure time: 59s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human kidney.
    The section was incubated with ab320648 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in human spleen.
    The section was incubated with ab320648 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in human lung squamous cell carcinoma.
    The section was incubated with ab320648 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling SFTPA1 + SFTPA2 with ab320648 at 1/1000 (0.511 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in Type II pneumonocyte of human lung (PMID:32049827).
    The section was incubated with ab320648 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648), expandable thumbnail

    Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: cerebellum, spleen, liver (PMID: 34181042).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control  (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-SFTPA1 + SFTPA2 antibody [EPR29098-6] (ab320648) at 1/1000 dilution

    Lane 1: Human lung tissue lysate at 20 µg

    Lane 2: Human cerebellum tissue lysate at 20 µg

    Lane 3: Human spleen tissue lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 28 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

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