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AB237804

Anti-SFXN4 antibody

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(1 Publication)

Rabbit Polyclonal SFXN4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SFXN4.

View Alternative Names

BCRM1, SFXN4, Sideroflexin-4, Breast cancer resistance marker 1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SFXN4 antibody (AB237804)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SFXN4 antibody (AB237804)

U-251 MG (human brain glioma cell line) cells stained for SFXN4 (Green) using ab237804 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFXN4 antibody (AB237804)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFXN4 antibody (AB237804)

Paraffin-embedded human skeletal muscle tissue stained for SFXN4 using ab237804 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFXN4 antibody (AB237804)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SFXN4 antibody (AB237804)

Paraffin-embedded human thymus tissue stained for SFXN4 using ab237804 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-SFXN4 antibody (AB237804)
  • WB

Supplier Data

Western blot - Anti-SFXN4 antibody (AB237804)

All lanes:

Western blot - Anti-SFXN4 antibody (ab237804) at 1/500 dilution

Lane 1:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) whole cell lysate

Lane 3:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate

Lane 4:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SFXN4.

Q6P4A7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sideroflexin 4 (SFXN4) also known as mitochondrial iron transporter SLC56A4 is part of the sideroflexin family involved with cellular iron transport within mitochondria. This protein has a molecular mass of approximately 36 kDa. Researchers often study SFXN4 due to its integral role in mitochondrial membranes where it regulates iron homeostasis. SFXN4 shows expression across various tissues with notable presence in heart skeletal muscle and pancreas indicating a widespread role in energy-demanding tissues.
Biological function summary

The SFXN4 protein participates in managing iron import into mitochondrian important for mitochondrial function and overall cellular metabolism. SFXN4 is part of a larger mitochondrial protein complex that includes sideroflexins which together ensure proper iron distribution. This function is essential in maintaining mitochondrial respiration and energy production as mitochondria require iron to produce heme and iron-sulfur clusters necessary for electron transport chain activity.

Pathways

The SFXN4 protein plays a significant role in mitochondrial iron import and homeostasis pathways. It closely interacts with proteins involved in the electron transport chain and is related to other mitochondrial transport systems. One key pathway includes the formation and maintenance of iron-sulfur clusters necessary for electron transport function where SFXN4 cooperates with other mitochondrial proteins like frataxin and ISCU which are vital for cluster assembly.

SFXN4 has implications in disorders where iron metabolism is disrupted such as sideroblastic anemia and Friedreich's ataxia. In these conditions faulty iron transport and mitochondrial iron overload contribute to pathogenesis. Relationships between SFXN4 and proteins like frataxin become important as frataxin deficiency in Friedreich's ataxia leads to impaired mitochondrial function similar to what might be seen with SFXN4 dysfunction. The links between SFXN4 and these disorders make it a potential target for therapeutic intervention and understanding mitochondrial-linked pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial amino-acid transporter (By similarity). Does not act as a serine transporter : not able to mediate transport of serine into mitochondria (PubMed : 30442778).
See full target information SFXN4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 13:45 PubMed36879298

2023

PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Chen,Wei Dong,Menghao Zhou,Chenlu Yang,Ming Xiong,Gallina Kazobinka,Zhaohui Chen,Yifei Xing,Teng Hou
View all publications
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Product promise

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