AB172971

Anti-SFXN5 antibody [EPR9532]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal SFXN5 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

Sideroflexin-5, SFXN5

1 Images

Supplier Data

Western blot - Anti-SFXN5 antibody [EPR9532] (AB172971)

All lanes:

Western blot - Anti-SFXN5 antibody [EPR9532] (ab172971) at 1/10000 dilution

Lane 1:

Human fetal kidney lysates at 10 µg

Lane 2:

Human fetal brain lysates at 10 µg

Lane 3:

SH-SY5Y lysates at 10 µg

Lane 4:

BxPC-3 lysates at 10 µg

Predicted band size: 37 kDa

false

Carrier free

Anti-SFXN5 antibody [EPR9532] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9532

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sideroflexin 5 commonly known as SFXN5 is a protein that acts mechanically as an inner mitochondrial membrane transporter. It belongs to the sideroflexin family of proteins and has a molecular weight of approximately 35 kDa. SFXN5 shows expression in various tissues including the heart brain and skeletal muscle reflecting its important function in cellular metabolism and energy production.

Biological function summary

SFXN5 contributes to the regulation of mitochondrial iron homeostasis and amino acid metabolism. This protein integrates into a larger complex involved in the translocation of specific substrates across the mitochondrial membrane. By modulating iron levels within mitochondria SFXN5 assists in maintaining cellular iron balance which is necessary for proper mitochondrial function and energy bioavailability.

Pathways

SFXN5 exerts influence on the heme synthesis and iron-sulfur cluster pathways. The transportation and regulation functions of SFXN5 relate closely to these pathways supporting the synthesis of heme groups and iron-sulfur clusters that are essential for numerous enzymatic processes. SFXN5 works in connection with proteins like frataxin and mitoferrin which also play roles in iron regulation and mitochondrial function.

SFXN5 has relevance to conditions like neurodegenerative diseases and anemia. Abnormalities in iron transport and homeostasis within mitochondria can contribute to the pathogenesis of these disorders. Proteins involved in related pathways such as ferritin and transferrin further connect with SFXN5 and are significant in understanding disease mechanisms involving disrupted iron metabolism. Understanding SFXN5's role in these contexts could offer insights into potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mitochondrial amino-acid transporter (By similarity). Transports citrate (By similarity). Does not act as a serine transporter : not able to mediate transport of serine into mitochondria (By similarity) (PubMed : 30442778). In brown adipose tissue, plays a role in the regulation of UCP1-dependent thermogenesis probably by supporting mitochondrial glycerol-3-phosphate utilization (By similarity).

See full target information SFXN5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell 185:4654-4673.e28 PubMed36334589

2022

Architecture of the outbred brown fat proteome defines regulators of metabolic physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Haopeng Xiao,Luiz H M Bozi,Yizhi Sun,Christopher L Riley,Vivek M Philip,Mandy Chen,Jiaming Li,Tian Zhang,Evanna L Mills,Margo P Emont,Wenfei Sun,Anita Reddy,Ryan Garrity,Jiani Long,Tobias Becher,Laura Potano Vitas,Dina Laznik-Bogoslavski,Martha Ordonez,Xinyue Liu,Xiong Chen,Yun Wang,Weihai Liu,Nhien Tran,Yitong Liu,Yang Zhang,Aaron M Cypess,Andrew P White,Yuchen He,Rebecca Deng,Heiko Schöder,Joao A Paulo,Mark P Jedrychowski,Alexander S Banks,Yu-Hua Tseng,Paul Cohen,Linus T Tsai,Evan D Rosen,Samuel Klein,Maria Chondronikola,Fiona E McAllister,Nick Van Bruggen,Edward L Huttlin,Bruce M Spiegelman,Gary A Churchill,Steven P Gygi,Edward T Chouchani

Neurochemical research 47:3428-3439 PubMed35904699

2022

Sex Differentially Alters Secretion of Brain Extracellular Vesicles During Aging: A Potential Mechanism for Maintaining Brain Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yohan Kim,Rocío Pérez-González,Chelsea Miller,Michelle Kurz,Pasquale D'Acunzo,Chris N Goulbourne,Efrat Levy

Scientific reports 12:9241 PubMed35655086

2022

FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain.

Applications

Unspecified application

Species

Unspecified reactive species

Diane M Bogdan,Keith Studholme,Adriana DiBua,Chris Gordon,Martha P Kanjiya,Mei Yu,Michelino Puopolo,Martin Kaczocha

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 41:2546-2560 PubMed33818185

2021

A large portion of the astrocyte proteome is dedicated to perivascular endfeet, including critical components of the electron transport chain.

Applications

Unspecified application

Species

Unspecified reactive species

Jesse A Stokum,Bosung Shim,Weiliang Huang,Maureen Kane,Jesse A Smith,Volodymyr Gerzanich,J Marc Simard

Cell stem cell 28:524-534.e7 PubMed33202244

2020

CRISPR-Mediated Induction of Neuron-Enriched Mitochondrial Proteins Boosts Direct Glia-to-Neuron Conversion.

Applications

Unspecified application

Species

Unspecified reactive species

Gianluca L Russo,Giovanna Sonsalla,Poornemaa Natarajan,Christopher T Breunig,Giorgia Bulli,Juliane Merl-Pham,Sabine Schmitt,Jessica Giehrl-Schwab,Florian Giesert,Martin Jastroch,Hans Zischka,Wolfgang Wurst,Stefan H Stricker,Stefanie M Hauck,Giacomo Masserdotti,Magdalena Götz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SFXN5 antibody [EPR9532]

Western blot - Anti-SFXN5 antibody [EPR9532] (AB172971)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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