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AB204367

Anti-SGF29 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal SGF29 antibody. N-terminal. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SGF29 aa 1-100.

View Alternative Names

CCDC101, SGF29, SAGA-associated factor 29, Coiled-coil domain-containing protein 101, SAGA complex-associated factor 29

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGF29 antibody - N-terminal (AB204367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGF29 antibody - N-terminal (AB204367)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling SGF29 with ab204367 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-SGF29 antibody - N-terminal (AB204367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SGF29 antibody - N-terminal (AB204367)

Immunofluorescence analysis of MCF7 cells labeling SGF29 with ab204367 at 4 μg/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SGF29 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96ES7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGF29 also known as SAGA-associated factor 29 is a component of the SAGA (Spt-Ada-GCN5 acetyltransferase) coactivator complex which is responsible for transcriptional regulation. The SGF29 protein has a molecular mass of approximately 60 kDa. Researchers have observed its expression in various tissues though it is more prominent in the nucleus where it exerts its functional roles. SGF29 interacts with histone H3 and recognizes the H3K4me3 mark which plays an important role in recruiting other SAGA components for transcription activation.
Biological function summary

The role of SGF29 involves it in chromatin remodeling and transcriptional activation processes. It acts as a scaffold protein binding to methylated histone H3 lysine residues and helps localize the SAGA complex to chromatin sites that are marked for transcription. By attaching to these regions SGF29 facilitates the acetylation of histones a modification that leads to the relaxation of chromatin structure more accessible to transcription machinery.

Pathways

Researchers describe SGF29 as part of the transcriptional regulation pathway and epigenetic modification processes. SGF29 is especially significant in pathways involving the acetylation of histones through the SAGA complex. It works closely with other proteins such as GCN5 and ADA2 which are also components of the SAGA complex. SGF29's interaction with these proteins ensures that acetylation proceeds efficiently influencing gene expression patterns.

SGF29 implications are evident in some cancer types and specific neurodevelopmental disorders. Scientists noticed that aberrant expression or mutations in SGF29 can correlate with tumorigenesis due to misregulated transcription processes. Additionally certain neurodevelopmental disorders can arise when there are disruptions in the pathways SGF29 is involved with potentially affecting cognitive function. These disorders sometimes link SGF29’s abnormal activity with misregulated histone modifications and the modulation of genes associated with neuronal development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin reader component of some histone acetyltransferase (HAT) SAGA-type complexes like the TFTC-HAT, ATAC or STAGA complexes (PubMed : 19103755, PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). SGF29 specifically recognizes and binds methylated 'Lys-4' of histone H3 (H3K4me), with a preference for trimethylated form (H3K4me3) (PubMed : 20850016, PubMed : 21685874, PubMed : 26421618, PubMed : 26578293). In the SAGA-type complexes, SGF29 is required to recruit complexes to H3K4me (PubMed : 20850016). Involved in the response to endoplasmic reticulum (ER) stress by recruiting the SAGA complex to H3K4me, thereby promoting histone H3 acetylation and cell survival (PubMed : 23894581). Also binds non-histone proteins that are methylated on Lys residues : specifically recognizes and binds CGAS monomethylated on 'Lys-506' (By similarity).
See full target information SGF29
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 12:e719 PubMed35092699

2022

Methionine oxidation of CLK4 promotes the metabolic switch and redox homeostasis in esophageal carcinoma via inhibiting MITF selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yaxing Shen,Heng Zhang,Shihua Yao,Feng Su,Hao Wang,Jun Yin,Yong Fang,Lijie Tan,Kaiguang Zhang,Xiangshan Fan,Ming Zhong,Qingxin Zhou,Jie He,Zhiyong Zhang
View all publications
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Product promise

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