Rabbit Polyclonal SGK3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SGK3 aa 1-300.
Images
Publications
Filter by
- Reproductive biology and endocrinology : RB&E 19:1672021Circ_0043532 regulates miR-182/SGK3 axis to promote granulosa cell progression in polycystic ovary syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLishuang Xu,Fang Xiong,Yinyang Bai,Juxia Xiao,Yun Zhang,Jie Chen,Qiuping LiPubMed 34740363
- Brazilian journal of otorhinolaryngology 85:705-7152018Serum- and glucocorticoid-inducible kinase 3 is a potential oncogene in nasopharyngeal carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Chen,Hai Liang Li,Bo Bo Li,Wei Li,Dong Ma,Yong He Li,Tao LiuPubMed 30108027
- Experimental and therapeutic medicine 12:1741-17492016Lovastatin exerts protective effects on endothelial cells via upregulation of PTK2B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Chu,Lili Guan,Dihua Huang,Yuezhong Ren,Yan ZhouPubMed 27602089
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SGK3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BR1
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Alternative names
CISK, SGKL, SGK3, Serine/threonine-protein kinase Sgk3, Cytokine-independent survival kinase, Serum/glucocorticoid-regulated kinase 3, Serum/glucocorticoid-regulated kinase-like
Recommended products
Rabbit Polyclonal SGK3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SGK3 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SGK3 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BR1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGK3 also known as Serum/Glucocorticoid Regulated Kinase Family Member 3 or CISK is a serine/threonine protein kinase with a molecular weight of approximately 56 kDa. It plays a role in the regulation of ion channels cell growth and survival by phosphorylating other proteins. SGK3 is expressed in various tissues including the brain kidney and liver. Expression is often responsive to changes in serum and glucocorticoid levels.
- Biological function summary
SGK3 contributes to cellular growth and metabolism. This protein integrates signals involved in the response to oxidative stress and plays a part in the cellular response to environmental changes. As a member of the AGC kinase family SGK3 shares structural and functional similarities with AKT another significant kinase involved in growth regulation and apoptosis control.
- Pathways
SGK3 functions within the PI3K/AKT signaling pathway and also interacts in the mTOR signaling network. These pathways are critical for regulating cell proliferation survival and metabolism. SGK3 can phosphorylate several downstream targets including FOXO3a and NDRG1 contributing to cellular resistance to stress and promoting survival pathways.
- Associated diseases and disorders
SGK3 has been linked to cancer and metabolic disorders. Its role in the PI3K/AKT pathway means that deregulated SGK3 activity can contribute to oncogenesis often through pathways shared with proteins such as AKT and mTOR. Aberrant SGK3 expression or function is also related to insulin resistance impacting glucose metabolism and associating it with contributors to type 2 diabetes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-SGK3 antibody (ab153981)
7.5% SDS PAGE
All lanes: Western blot - Anti-SGK3 antibody (ab153981) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 57 kDa, 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SGK3 antibody (ab153981)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling SGK3 with ab153981 at 1/200 dilution. Lower panel is costained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGK3 antibody (ab153981)
Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor tissue labeling SGK3 with ab153981 at 1/500 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com