Anti-SGLT1 antibody

• WB

Unknown

Western blot - Anti-SGLT1 antibody (AB14686)

Tissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1 : 500 diluted ab14686.

The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.

All lanes:

Western blot - Anti-SGLT1 antibody (ab14686) at 1/500 dilution

Lane 1:

Rat enterocytes

Lane 2:

Rat heart tissue

Lane 3:

Human skeletal muscle tissue

Predicted band size: 73 kDa

false

• WB

CiteAb

Western blot - Anti-SGLT1 antibody (AB14686)

SGLT1 western blot using anti-SGLT1 antibody ab14686. Publication image and figure legend from Wang, J., Wang, Z., et al., 2020, Aging (Albany NY), PubMed 32357142.

ab14686 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab14686 please see the product overview.

Evaluation of the effects of ACSL4 on O-GlcNAc-mediated HCC growth. Huh-7 and SK-HEP-1 cells were transfected with OE-ACSL4 or si-ACSL4, and then the cells were harvested for the western blot assay to detect the expression of the following proteins. (A) OGT and O-GlcNAc in Huh-7 cells. (B) UAP1, GLUT1, GLUT2, GUCY1A3, CANT1 and SGLT1 in Huh-7 cells. (C, D) OGT, O-GlcNAc, UAP1, GLUT1, GLUT2, GUCY1A3, CANT1 and SGLT1 in SK-HEP-1 cells. (E) IP assay used to detect the interaction between O-GlcNAc and ACSL4 with an antibody against O-GlcNAc or ACSL4. IgG served as a negative control. Then, the si-ACSL4-transfected or untransfected Huh-7 and SK-HEP-1 cells were treated with PUGNAc, GlcNAc or nothing, and the following assays were carried out. (F, G) The levels of ACSL4, O-GlcNAc, mTOR and p-mTOR were determined by using a western blot assay. (H, I) The protein stability was determined by western blot after incubation with CHX (100 μg/ml) for 0, 1, 2, 4, 8 or 24 hours. (J, K) Cell proliferation was detected by CCK-8 assay. (L) Cell apoptosis was assessed by flow cytometry assay. (A–D) *p<0.05, si-ACSL4/OE-ACSL4 group compared with control group; (E–L)*p<0.05, PUGNAc/GlcNAc group compared with control group; #p<0.05, PUGNAc + si-ACSL4/GlcNAc + si-ACSL4 group compared with PUGNAc/GlcNAc group).

false