Rabbit Polyclonal SGLT1 antibody. Suitable for Dot, ELISA, IP, WB and reacts with Purified native protein, Human, Rat samples. Cited in 67 publications. Immunogen corresponding to Synthetic Peptide within Human SLC5A1 aa 400-450.
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- PeerJ 11:e156472023Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianyi Li,Lingbing Meng,Dishan Wu,Hongxuan Xu,Xing Hu,Gaifeng Hu,Yuhui Chen,Jiapei Xu,Tao Gong,Deping LiuPubMed 37663275
- Scientific reports 13:108382023Regulatory mechanisms of glucose absorption in the mouse proximal small intestine during fasting and feeding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChisato Nakamura,Noriko Ishizuka,Kanako Yokoyama,Yuyu Yazaki,Fumiya Tatsumi,Naotaka Ikumi,Wendy Hempstock,Akira Ikari,Yuta Yoshino,Hisayoshi HayashiPubMed 37407613
- Biology 12:2023Alleviative Effect of Probiotic Ferment on Infection in Piglets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Xu,Yong Guo,Yuanyuan Zhang,Kai Cao,Xinchen Zhou,Mengqi Qian,Xinyan HanPubMed 37372164
- Cardiovascular diabetology 22:242023Targeting high glucose-induced epigenetic modifications at cardiac level: the role of SGLT2 and SGLT2 inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucia Scisciola,Fatemeh Taktaz,Rosaria Anna Fontanella,Ada Pesapane, Surina,Vittoria Cataldo,Puja Ghosh,Martina Franzese,Armando Puocci,Pasquale Paolisso,Concetta Rafaniello,Raffaele Marfella,Maria Rosaria Rizzo,Emanuele Barbato,Marc Vanderheyden,Michelangela BarbieriPubMed 36732760
- iScience 26:1060572023Oral metformin transiently lowers post-prandial glucose response by reducing the apical expression of sodium-glucose co-transporter 1 in enterocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorea Zubiaga,Olivier Briand,Florent Auger,Veronique Touche,Thomas Hubert,Julien Thevenet,Camille Marciniak,Audrey Quenon,Caroline Bonner,Simon Peschard,Violeta Raverdy,Mehdi Daoudi,Julie Kerr-Conte,Gianni Pasquetti,Hermann Koepsell,Daniela Zdzieblo,Markus Mühlemann,Bernard Thorens,Nathalie D Delzenne,Laure B Bindels,Benoit Deprez,Marie C Vantyghem,Blandine Laferrère,Bart Staels,Damien Huglo,Sophie Lestavel,François PattouPubMed 36942050
- Nutrients 14:2022γ-Conglutin: New Findings about Its Action at the Intestinal Barrier and a Critical Analysis of the State of the Art on Its Postprandial Glycaemic Regulating Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuditta C Heinzl,Marco Tretola,Stefano De Benedetti,Paolo Silacci,Alessio ScarafoniPubMed 36079925
- Journal of clinical medicine 11:2022Loss of Serum Glucocorticoid-Inducible Kinase 1 SGK1 Worsens Malabsorption and Diarrhea in Microvillus Inclusion Disease (MVID).Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Kaimul Ahsan,Diego Carlos Dos Reis,Andrea Barbieri,Kaelyn D Sumigray,Timothy Nottoli,Pedro J Salas,Nadia A AmeenPubMed 35887942
- Pharmaceutical biology 60:1237-12542022Comparative study on the gastrointestinal- and immune- regulation functions of Hedysari Radix Paeparata Cum Melle and Astragali Radix Praeparata cum Melle in rats with spleen-qi deficiency, based on fuzzy matter-element analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYugui Zhang,Jiangtao Niu,Shujuan Zhang,Xinlei Si,Tian-Tian Bian,Hongwei Wu,Donghui Li,Yujing Sun,Jing Jia,Erdan Xin,Xingke Yan,Yuefeng LiPubMed 35763552
- Endocrinology 163:2022Enterocyte HKDC1 Modulates Intestinal Glucose Absorption in Male Mice Fed a High-fat Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoseph L Zapater,Barton Wicksteed,Brian T LaydenPubMed 35435980
- Molecular and cellular biochemistry 477:1865-18722022Empagliflozin inhibits angiotensin II-induced hypertrophy in H9c2 cardiomyoblasts through inhibition of NHE1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNabeel Abdulrahman,Meram Ibrahim,Jensa Mariam Joseph,Hanan Mahmoud Elkoubatry,Al-Anood Al-Shamasi,Menatallah Rayan,Alain Pierre Gadeau,Rashid Ahmed,Hussein Eldassouki,Anwarul Hasan,Fatima MraichePubMed 35334035
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: HEPES, Tris glycine, 30% Glycerol (glycerin, glycerine), 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLC5A1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P13866
Reactivity data
Select an application| Dot | ELISA | IP | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Predicted | Expected | Expected | Tested | Not recommended |
| Rat | Predicted | Expected | Expected | Tested | Not recommended |
| Purified native protein | Expected | Not recommended | Not recommended | Not recommended | Not recommended |
Dot
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Purified native protein | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose or D-galactose at the plasma membrane, with a Na(+) to sugar coupling ratio of 2:1. Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed:20980548, PubMed:34880492, PubMed:35077764, PubMed:8563765, PubMed:37217492). Has a primary role in the transport of dietary monosaccharides from enterocytes to blood. Responsible for the absorption of D-glucose or D-galactose across the apical brush-border membrane of enterocytes, whereas basolateral exit is provided by GLUT2. Additionally, functions as a D-glucose sensor in enteroendocrine cells, triggering the secretion of the incretins GCG and GIP that control food intake and energy homeostasis (By similarity) (PubMed:8563765). Together with SGLT2, functions in reabsorption of D-glucose from glomerular filtrate, playing a nonredundant role in the S3 segment of the proximal tubules (By similarity). Transports D-glucose into endometrial epithelial cells, controlling glycogen synthesis and nutritional support for the embryo as well as the decidual transformation of endometrium prior to conception (PubMed:28974690). Acts as a water channel enabling passive water transport across the plasma membrane in response to the osmotic gradient created upon sugar and Na(+) uptake. Has high water conductivity, comparable to aquaporins, and therefore is expected to play an important role in transepithelial water permeability, especially in the small intestine.
Alternative names
NAGT, SGLT1, SLC5A1, Sodium/glucose cotransporter 1, Na(+)/glucose cotransporter 1, High affinity sodium-glucose cotransporter, Solute carrier family 5 member 1
Recommended products
Rabbit Polyclonal SGLT1 antibody. Suitable for Dot, ELISA, IP, WB and reacts with Purified native protein, Human, Rat samples. Cited in 67 publications. Immunogen corresponding to Synthetic Peptide within Human SLC5A1 aa 400-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: HEPES, Tris glycine, 30% Glycerol (glycerin, glycerine), 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLC5A1 aa 400-450. The exact immunogen used to generate this antibody is proprietary information. Database link P13866
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified on antigen sepharose affinity column
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
A blocking peptide for this product is available as ab190911.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGLT1 also known as sodium/glucose cotransporter 1 or SLC5A1 is a membrane protein actively responsible for glucose and galactose uptake in the small intestine and renal tubules. It has a molecular mass of approximately 73 kDa. Expressed mainly in the brush border of the enterocytes in the small intestinal lining and to a lesser degree in the kidney SGLT1 functions by coupling the transport of glucose to sodium ions' electrochemical gradient enabling effective nutrient absorption.
- Biological function summary
SGLT1's main role is in facilitating nutrient absorption in the gastrointestinal tract. It operates as part of the larger SGLT protein family but does not form a complex with other proteins. The protein is essential for the transport of monosaccharides from the gut into circulation. By ensuring glucose and galactose absorption during digestive processes SGLT1 provides necessary carbohydrates for cellular metabolism and systemic energy homeostasis.
- Pathways
The transport protein SGLT1 is integral to the glucose uptake pathway. This pathway intersects with the insulin signaling pathway because the glucose absorbed by SGLT1 later enters systemic circulation. SGLT1 also complements the GLUT family of transporters especially GLUT2 which function downstream by facilitating glucose transport across the intestinal basolateral membrane into the bloodstream. The interplay between these transport mechanisms maintains glucose levels post-ingestion.
- Associated diseases and disorders
SGLT1 dysfunction is linked to glucose-galactose malabsorption a genetic disorder causing severe dehydration and diarrhea due to glucose and galactose accumulation in the lumen. Additionally research investigates SGLT1's contribution to diabetes management given its role in glucose absorption. The protein's activity along with related proteins like GLUT2 and GLUT4 could influence insulin resistance and glucose homeostasis in metabolic syndromes. Understanding SGLT1 in these contexts enables potential therapeutic interventions for conditions involving disordered glucose metabolism.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SGLT1 antibody (ab14686)
Tissue extracts were made in Solobuffer with protease inhibitors. The samples were solubilized in SDS-PAGE sample buffer and reduced with 2.5% BME.
Total 40-50ug protein was applied per lane on 10% SDS PAGE gels.
The proteins were transferred to nitrocellulose F and blocked with 5% milk and blotted with 1:500 diluted ab14686.The image was obtained with high sensitivity ECL assay and Image was captured with a CCD cooled camera for 30 sec.
All lanes: Western blot - Anti-SGLT1 antibody (ab14686) at 1/500 dilution
Lane 1: Rat enterocytes
Lane 2: Rat heart tissue
Lane 3: Human skeletal muscle tissue
Predicted band size: 73 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-SGLT1 antibody (ab14686)
SGLT1 western blot using anti-SGLT1 antibody ab14686. Publication image and figure legend from Wang, J., Wang, Z., et al., 2020, Aging (Albany NY), PubMed 32357142.
ab14686 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab14686 please see the product overview.
Evaluation of the effects of ACSL4 on O-GlcNAc-mediated HCC growth. Huh-7 and SK-HEP-1 cells were transfected with OE-ACSL4 or si-ACSL4, and then the cells were harvested for the western blot assay to detect the expression of the following proteins. (A) OGT and O-GlcNAc in Huh-7 cells. (B) UAP1, GLUT1, GLUT2, GUCY1A3, CANT1 and SGLT1 in Huh-7 cells. (C, D) OGT, O-GlcNAc, UAP1, GLUT1, GLUT2, GUCY1A3, CANT1 and SGLT1 in SK-HEP-1 cells. (E) IP assay used to detect the interaction between O-GlcNAc and ACSL4 with an antibody against O-GlcNAc or ACSL4. IgG served as a negative control. Then, the si-ACSL4-transfected or untransfected Huh-7 and SK-HEP-1 cells were treated with PUGNAc, GlcNAc or nothing, and the following assays were carried out. (F, G) The levels of ACSL4, O-GlcNAc, mTOR and p-mTOR were determined by using a western blot assay. (H, I) The protein stability was determined by western blot after incubation with CHX (100 μg/ml) for 0, 1, 2, 4, 8 or 24 hours. (J, K) Cell proliferation was detected by CCK-8 assay. (L) Cell apoptosis was assessed by flow cytometry assay. (A–D) *P<0.05, si-ACSL4/OE-ACSL4 group compared with control group; (E–L)*P<0.05, PUGNAc/GlcNAc group compared with control group; #P<0.05, PUGNAc + si-ACSL4/GlcNAc + si-ACSL4 group compared with PUGNAc/GlcNAc group).
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