AB58298

Anti-SGLT2 antibody [3G8]

Name: Anti-SGLT2 antibody [3G8] (ab58298) | Abcam
Brand: Abcam Limited
SKU: AB58298
Price: 570 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Mouse Monoclonal SGLT2 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC5A2 aa 200-300.

View Alternative Names

SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2

2 Images

Flow Cytometry - Anti-SGLT2 antibody [3G8] (AB58298)

Flow Cyt

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Flow Cytometry - Anti-SGLT2 antibody [3G8] (AB58298)

Overlay histogram showing HEK293 cells stained with ab58298 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58298, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

This image was generated using the ascites version of the product.

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Western blot - Anti-SGLT2 antibody [3G8] (AB58298)

Western blot against tagged recombinant protein immunogen using ab58298 SGLT2 antibody at 1ug/ml. Predicted band size of immunogen is 32 kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-SGLT2 antibody [3G8] (ab58298)

Predicted band size: 73 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G8

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human SLC5A2 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

P31639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGLT2 also known as sodium-glucose co-transporter 2 is a protein responsible for reabsorbing glucose in the renal proximal convoluted tubule. It carries glucose along with sodium ions across cell membranes enabling glucose reabsorption from the glomerular filtrate back into the bloodstream a process essential for the maintenance of glucose homeostasis. This protein has a molecular weight of approximately 75 kDa. SGLT2 is mostly expressed in the renal epithelial cells located within the kidney's proximal tubule.

Biological function summary

Sodium-glucose co-transporter 2 plays a central role in renal glucose reabsorption. It operates as part of a vital mechanism in the kidney where it engages in the active transport of glucose against its concentration gradient facilitated by the sodium gradient produced by the Na⁺/K⁺-ATPase pump. This transporter ensures minimal loss of glucose in urine maintaining energy balance and blood glucose levels.

Pathways

SGLT2 is involved in glucose and sodium transport pathways critical for glycolytic and gluconeogenic processes. These pathways are key for energy production and glucose homeostasis. It works closely with proteins like GLUT2 which operates in the basolateral membrane of renal tubular cells to further transfer reabsorbed glucose into the bloodstream. Together these proteins form a tightly regulated network that maintains normal blood glucose levels.

SGLT2 has a significant impact on diabetes and cardiovascular disease. Inhibitors of SGLT2 are effective treatments for type 2 diabetes by preventing glucose reabsorption leading to increased glucose excretion through urine and improved glycemic control. The glucose-lowering effect also contributes to reducing risks associated with cardiovascular diseases. In this context SGLT2 interacts with protein targets like insulin where its modulation offers therapeutic efficacy in managing hyperglycemia.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1 : 1 (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493). Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity (PubMed : 37217492). Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (By similarity).

See full target information SLC5A2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular imaging and biology 27:558-569 PubMed40659959

2025

Preclinical Evaluation of Novel SGLT2-Targeted Near-Infrared Optical Imaging Agent for Early-Stage Pulmonary Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Ortmeyer Welch,Kelly Anne McGovern,Lydia Chen,Ryan Krouse,Kevin Guo,Jeffrey Huang,Michael Brown,Jake Mlakar,Venu Bandi,David Holt,Paul Zhang,Sunil Singhal

Bioscience reports 44: PubMed38747277

2024

Co-localization of the sodium-glucose co-transporter-2 channel (SGLT-2) with endothelin ETA and ETB receptors in human cardiorenal tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas L Williams,Rhoda E Kuc,Anna L Paterson,George R Abraham,Anna L Pullinger,Janet J Maguire,Sanjay Sinha,Peter J Greasley,Philip Ambery,Anthony P Davenport

Cell proliferation 55:e13190 PubMed35102634

2022

Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system.

Applications

Unspecified application

Species

Unspecified reactive species

Thao Thi Thanh Ngo,Bella Rossbach,Isabelle Sébastien,Julia C Neubauer,Andreas Kurtz,Krithika Hariharan

Endocrinology 161: PubMed32428240

2020

Dapagliflozin Does Not Directly Affect Human α or β Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chunhua Dai,John T Walker,Alena Shostak,Yasir Bouchi,Greg Poffenberger,Nathaniel J Hart,David A Jacobson,M Wade Calcutt,Rita Bottino,Dale L Greiner,Leonard D Shultz,Owen P McGuinness,E Danielle Dean,Alvin C Powers

Medical science monitor : international medical jo 23:3737-3745 PubMed28763435

2017

Therapeutic Effect of Sodium Glucose Co-Transporter 2 Inhibitor Dapagliflozin on Renal Cell Carcinoma.

Applications

Species

Human

Haoyu Kuang,Liya Liao,Hongtao Chen,Qian Kang,Xiaochun Shu,Yanan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGLT2 antibody [3G8]

Flow Cytometry - Anti-SGLT2 antibody [3G8] (AB58298)

Western blot - Anti-SGLT2 antibody [3G8] (AB58298)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise