AB37296

Anti-SGLT2 antibody

Name: Anti-SGLT2 antibody (ab37296) | Abcam
Brand: Abcam Limited
SKU: AB37296
Price: 210 USD
Availability: InStock
Rating: 4 (13 reviews)

(13 Reviews)

(51 Publications )

Anti-SGLT2 antibody (ab37296) is a rabbit polyclonal antibody detecting SGLT2 in Western Blot. Suitable for Human, Mouse, Rat.

- Over 40 publications
- Trusted since 2006

1 Images

Supplier Data

Western blot - Anti-SGLT2 antibody (AB37296)

Blocking buffer : 3% milk

All lanes:

Western blot - Anti-SGLT2 antibody (ab37296) at 1 µg/mL

Lane 1:

Jurkat at 20 µg

Lanes 2 - 3:

NIH3T3 at 20 µg

Secondary

All lanes:

Goat Polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Rat

Applications

applications

Immunogen

Synthetic Peptide within Mouse Slc5a2. The exact immunogen used to generate this antibody is proprietary information.

Q923I7

Specificity

The antibody detects 74 kDa SGLT-2 from human, mouse, and rat origins. Reactivity to other species has not been determined. A ~55 kDa and a ~45 kDa band can also be detected, representing the SGLT-2 cleavage fragments.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-SGLT2 antibody (ab37296) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of SGLT2?
Anti-SGLT2 (ab37296) specifically detects a band for SGLT2 (UniProt: Q923I7) at a molecular weight of 73kDa.

Trusted by the scientific community
Anti-SGLT2 (ab37296) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-SGLT2 (ab37296) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGLT2 also known as sodium-glucose co-transporter 2 is a protein responsible for reabsorbing glucose in the renal proximal convoluted tubule. It carries glucose along with sodium ions across cell membranes enabling glucose reabsorption from the glomerular filtrate back into the bloodstream a process essential for the maintenance of glucose homeostasis. This protein has a molecular weight of approximately 75 kDa. SGLT2 is mostly expressed in the renal epithelial cells located within the kidney's proximal tubule.

Biological function summary

Sodium-glucose co-transporter 2 plays a central role in renal glucose reabsorption. It operates as part of a vital mechanism in the kidney where it engages in the active transport of glucose against its concentration gradient facilitated by the sodium gradient produced by the Na?/K?-ATPase pump. This transporter ensures minimal loss of glucose in urine maintaining energy balance and blood glucose levels.

Pathways

SGLT2 is involved in glucose and sodium transport pathways critical for glycolytic and gluconeogenic processes. These pathways are key for energy production and glucose homeostasis. It works closely with proteins like GLUT2 which operates in the basolateral membrane of renal tubular cells to further transfer reabsorbed glucose into the bloodstream. Together these proteins form a tightly regulated network that maintains normal blood glucose levels.

SGLT2 has a significant impact on diabetes and cardiovascular disease. Inhibitors of SGLT2 are effective treatments for type 2 diabetes by preventing glucose reabsorption leading to increased glucose excretion through urine and improved glycemic control. The glucose-lowering effect also contributes to reducing risks associated with cardiovascular diseases. In this context SGLT2 interacts with protein targets like insulin where its modulation offers therapeutic efficacy in managing hyperglycemia.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1 : 1 (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493). Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity (PubMed : 37217492). Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (By similarity).

See full target information SLC5A2

Alternative Names

SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2

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Publications (51)

Recent publications for all applications. Explore the full list and refine your search

Diabetes & metabolism journal 49:194-209 PubMed39721589

2024

Kidney Gastrin/CCKBR Attenuates Type 2 Diabetes Mellitus by Inhibiting SGLT2-Mediated Glucose Reabsorption through Erk/NF-κB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Zhang,Yuhan Zhang,Yang Shi,Dou Shi,Min Niu,Xue Liu,Xing Liu,Zhiwei Yang,Xianxian Wu

Physiological reports 12:e70121 PubMed39523534

2024

Uninephrectomy and sodium-glucose cotransporter 2 inhibitor administration delay the onset of hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuri Sakai Ishizaki,Masao Kikuchi,Koichi Kaikita,Shouichi Fujimoto

Journal of cachexia, sarcopenia and muscle 14:2866-2881 PubMed37941098

2023

Improved endurance capacity of diabetic mice during SGLT2 inhibition: Role of AICARP, an AMPK activator in the soleus.

Applications

Unspecified application

Species

Unspecified reactive species

Shintaro Nakamura,Yasutaka Miyachi,Akihito Shinjo,Hisashi Yokomizo,Masatomo Takahashi,Kohta Nakatani,Yoshihiro Izumi,Hiroko Otsuka,Naoichi Sato,Ryuichi Sakamoto,Takashi Miyazawa,Takeshi Bamba,Yoshihiro Ogawa

PeerJ 11:e15647 PubMed37663275

2023

Adrenal SGLT1 or SGLT2 as predictors of atherosclerosis under chronic stress based on a computer algorithm.

Applications

Unspecified application

Species

Unspecified reactive species

Jianyi Li,Lingbing Meng,Dishan Wu,Hongxuan Xu,Xing Hu,Gaifeng Hu,Yuhui Chen,Jiapei Xu,Tao Gong,Deping Liu

BMC nephrology 24:250 PubMed37612668

2023

Knockdown of TMEM30A in renal tubular epithelial cells leads to reduced glucose absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Sipei Chen,Xinrou Song,Qiong Xiao,Li Wang,Xianjun Zhu,Yang Zou,Guisen Li

International journal of molecular sciences 24: PubMed37175762

2023

Sex Modulates Response to Renal-Tubule-Targeted Insulin Receptor Deletion in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Soha Sohail,Gabriella Akkawi,Taylor Rechter,Maurice B Fluitt,Carolyn M Ecelbarger

Nature communications 13:7918 PubMed36564419

2022

Glucose absorption drives cystogenesis in a human organoid-on-chip model of polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sienna R Li,Ramila E Gulieva,Louisa Helms,Nelly M Cruz,Thomas Vincent,Hongxia Fu,Jonathan Himmelfarb,Benjamin S Freedman

Nature communications 13:6081 PubMed36241632

2022

The gut microbe Bacteroides fragilis ameliorates renal fibrosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhou,Wen-Hui Wu,Zi-Lin Si,Hui-Ling Liu,Hanyu Wang,Hong Jiang,Ya-Fang Liu,Raphael N Alolga,Cheng Chen,Shi-Jia Liu,Xue-Yan Bian,Jin-Jun Shan,Jing Li,Ning-Hua Tan,Zhi-Hao Zhang

Frontiers in cardiovascular medicine 9:956041 PubMed36017090

2022

Empagliflozin prevents neointima formation by impairing smooth muscle cell proliferation and accelerating endothelial regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jochen Dutzmann,Lena Marie Bode,Katrin Kalies,Laura Korte,Kai Knöpp,Frederik Julius Kloss,Mirja Sirisko,Claudia Pilowski,Susanne Koch,Heiko Schenk,Jan-Marcus Daniel,Johann Bauersachs,Daniel G Sedding

Theranostics 12:5034-5050 PubMed35836807

2022

SGLT2 inhibitor dapagliflozin reduces endothelial dysfunction and microvascular damage during cardiac ischemia/reperfusion injury through normalizing the XO-SERCA2-CaMKII-coffilin pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ma,Rongjun Zou,Wanting Shi,Na Zhou,Shaoxian Chen,Hao Zhou,Xinxin Chen,Yueheng Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SGLT2 antibody

Western blot - Anti-SGLT2 antibody (AB37296)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (51)

Product promise