Anti-SGLT2 antibody [EPR27112-7] 20 ul size (ab306558)

Rabbit Recombinant Monoclonal SGLT2 antibody. Suitable for IHC-P, IHC-Fr, WB and reacts with Mouse, Rat samples.

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Images

Western blot - Anti-SGLT2 antibody [EPR27112-7] (AB306558), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	IHC-P	IHC-Fr	WB
Human	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Not recommended	Tested	Tested	Tested
Rat	Not recommended	Tested	Tested	Not recommended

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/1000	Notes -
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Rat	Dilution info -	Notes -

Target data

See full target information Slc5a2

Function

Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1 (By similarity). Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (By similarity). Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity (By similarity). Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (PubMed:20616166).

Alternative names

Sglt2, Slc5a2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2

Recommended products

Rabbit Recombinant Monoclonal SGLT2 antibody. Suitable for IHC-P, IHC-Fr, WB and reacts with Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR27112-7
Purification technique: Affinity purification Protein A
Specificity: This antibody is unsuitable for rat in western blot and human in IHC-P.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

SGLT2 also known as sodium-glucose co-transporter 2 is a protein responsible for reabsorbing glucose in the renal proximal convoluted tubule. It carries glucose along with sodium ions across cell membranes enabling glucose reabsorption from the glomerular filtrate back into the bloodstream a process essential for the maintenance of glucose homeostasis. This protein has a molecular weight of approximately 75 kDa. SGLT2 is mostly expressed in the renal epithelial cells located within the kidney's proximal tubule.

Biological function summary

Sodium-glucose co-transporter 2 plays a central role in renal glucose reabsorption. It operates as part of a vital mechanism in the kidney where it engages in the active transport of glucose against its concentration gradient facilitated by the sodium gradient produced by the Na⁺/K⁺-ATPase pump. This transporter ensures minimal loss of glucose in urine maintaining energy balance and blood glucose levels.

Pathways

SGLT2 is involved in glucose and sodium transport pathways critical for glycolytic and gluconeogenic processes. These pathways are key for energy production and glucose homeostasis. It works closely with proteins like GLUT2 which operates in the basolateral membrane of renal tubular cells to further transfer reabsorbed glucose into the bloodstream. Together these proteins form a tightly regulated network that maintains normal blood glucose levels.

Associated diseases and disorders

SGLT2 has a significant impact on diabetes and cardiovascular disease. Inhibitors of SGLT2 are effective treatments for type 2 diabetes by preventing glucose reabsorption leading to increased glucose excretion through urine and improved glycemic control. The glucose-lowering effect also contributes to reducing risks associated with cardiovascular diseases. In this context SGLT2 interacts with protein targets like insulin where its modulation offers therapeutic efficacy in managing hyperglycemia.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Samples are non-boiled as boiling may cause protein aggregation.

Blocking / Diluting buffer and concentration: 5% NFDM/TBST.

Exposure time: 15 seconds.
All lanes: Western blot - Anti-SGLT2 antibody [EPR27112-7] (ab306558) at 1/1000 dilution
Lane 1: Mouse kidney membrane fraction 20 μg
Lane 2: Mouse kidney cytoplasmic fraction 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 73 kDa
Exposure time: 15s
Western blot - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Blocking / Diluting buffer and concentration: 5% NFDM/TBST

Negative control: mouse pancreas (PMID: 30416006).

Samples are non-boiled as boiling may cause protein aggregation.
All lanes: Western blot - Anti-SGLT2 antibody [EPR27112-7] (ab306558) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate 20 μg
Lane 2: Mouse pancreas tissue lysate 20 μg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 73 kDa
Exposure time: 15s
Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse kidney (fresh) tissue labeling SGLT2 with ab306558 at 1/1000 (0.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining in the proximal tubules of mouse kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab306558 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas (fresh) tissue labeling SGLT2 with ab306558 at 1/1000 (0.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control: confocal image showing no staining in mouse pancreas (PMID: 33815487). The nuclear counterstain was DAPI (Blue). The section was incubated with ab306558 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat pancreas (fresh) tissue labeling SGLT2 with ab306558 at 1/1000 (0.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Negative control: confocal image showing no staining in rat pancreas (PMID: 33815487). The nuclear counterstain was DAPI (Blue). The section was incubated with ab306558 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution.
Immunohistochemistry - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SGLT2 with ab306558 at 1/5000 (0.104 μg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).

Positive staining on the brush border of rat proximal tubules.

The section was incubated with ab306558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 min.
Immunohistochemistry - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of paraffin-embedded transfected HEK-293T cells labeling SGLT2 with ab306558 at 1/5000 (0.104 μg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on HEK-293T cells transfected with a mouse SGLT2 expression vector (Panel A); No staining on HEK-293T cells transfected with a mouse Slc5a1 expression vector (Panel B); No staining on HEK-293T cells transfected with a mouse Slc5a3 expression vector (Panel C); No staining on HEK-293T cells transfected with an empty vector (Panel D). The section was incubated with ab306558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney (fresh) tissue labeling SGLT2 with ab306558 at 1/1000 (0.52 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). Confocal image showing positive staining in the proximal tubules of rat kidney. The nuclear counterstain was DAPI (Blue). The section was incubated with ab306558 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 (2 ug/mL) dilution.
Immunohistochemistry - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling SGLT2 with ab306558 at 1/5000 (0.104 μg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).

Positive staining on the brush border of mouse proximal tubules.

The section was incubated with ab306558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Primary diluent was used instead of primary antibody, followed by anti-Mouse IgG1 antibody (Rabbit monoclonal [M1gG51-4] Anti-Mouse IgG1 H&L ab125913).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 min.
Immunohistochemistry - Anti-SGLT2 antibody [EPR27112-7] (ab306558)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue to label SGLT2 with ab306558 at 1/5000 (0.104 μg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection kit).

Negative control: no staining on mouse pancreas.

The section was incubated with ab306558 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 min.