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AB320086

Anti-SGLT2 antibody [EPR29167-46]

  • BOND RX™ Validated
  • 20ul selling size
  • Recombinant
  • Advanced Validation
  • RabMAb
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Rabbit Recombinant Monoclonal SGLT2 antibody. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human samples.

View Alternative Names

SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SGLT2 with ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human pancreas.
The section was incubated with ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a SLC5A2 expression vector containing a his tag and (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling SGLT2 with ab320086 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a SLC5A2 expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag.
The section was incubated with ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SGLT2 with ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on human cerebrum.
The section was incubated with ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Multiplex immunohistochemistry - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab320086 anti-SLC5A2 used at 1 : 1000 (0.508 ug/ml) dilution and ab307969 anti-Aquaporin 3 used at a 1 : 5000 (0.0941 ug/ml) dilution.

Panel A : merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-Aquaporin 3 (gray; Opal™570) on human kidney.
Panel B : anti-PTPRP staining glomerulus in human kidney.
Panel C : anti-SLC5A2 staining proximal tubules in human kidney.
Panel D : anti-Aquaporin 3 staining collecting tubules in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab320086 and ab307969 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Multiplex immunohistochemistry - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human kidney tissue staining GLEPP1/PTPRO with ab322047 at a 1 : 2000 (0.261 ug/ml) dilution, ab320086 anti-SLC5A2 used at 1 : 1000 (0.508 ug/ml) dilution and ab316191 anti-SLC12A3 used at a 1 : 100 (5.08 ug/ml) dilution.

Panel A : merged staining of anti-PTPRP (magenta; Opal™690), anti-SLC5A2 (green; Opal™520) and anti-SLC12A3 (gray; Opal™570) on human kidney.
Panel B : anti-PTPRP staining glomerulus in human kidney.
Panel C : anti-SLC5A2 staining proximal tubules in human kidney.
Panel D : anti-SLC12A3 staining distal tubules in human kidney.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322047, ab320086 and ab316191 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] (AB320086)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SGLT2 with ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human kidney (PMID : 32488949).
The section was incubated with ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29167-46

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

mIHC, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen shares no sequence similarity with SLC5A1. Based on the immunogen sequence, we do not expect this antibody to cross-react with SLC5A1. No cross-reactivity testing has been performed.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/1000", "mIHC-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Transfected cell line - Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SGLT2 also known as sodium-glucose co-transporter 2 is a protein responsible for reabsorbing glucose in the renal proximal convoluted tubule. It carries glucose along with sodium ions across cell membranes enabling glucose reabsorption from the glomerular filtrate back into the bloodstream a process essential for the maintenance of glucose homeostasis. This protein has a molecular weight of approximately 75 kDa. SGLT2 is mostly expressed in the renal epithelial cells located within the kidney's proximal tubule.
Biological function summary

Sodium-glucose co-transporter 2 plays a central role in renal glucose reabsorption. It operates as part of a vital mechanism in the kidney where it engages in the active transport of glucose against its concentration gradient facilitated by the sodium gradient produced by the Na⁺/K⁺-ATPase pump. This transporter ensures minimal loss of glucose in urine maintaining energy balance and blood glucose levels.

Pathways

SGLT2 is involved in glucose and sodium transport pathways critical for glycolytic and gluconeogenic processes. These pathways are key for energy production and glucose homeostasis. It works closely with proteins like GLUT2 which operates in the basolateral membrane of renal tubular cells to further transfer reabsorbed glucose into the bloodstream. Together these proteins form a tightly regulated network that maintains normal blood glucose levels.

SGLT2 has a significant impact on diabetes and cardiovascular disease. Inhibitors of SGLT2 are effective treatments for type 2 diabetes by preventing glucose reabsorption leading to increased glucose excretion through urine and improved glycemic control. The glucose-lowering effect also contributes to reducing risks associated with cardiovascular diseases. In this context SGLT2 interacts with protein targets like insulin where its modulation offers therapeutic efficacy in managing hyperglycemia.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1 : 1 (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493, PubMed : 37217492, PubMed : 38057552). Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed : 20980548, PubMed : 28592437, PubMed : 34880493). Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity (PubMed : 37217492). Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (By similarity).
See full target information SLC5A2
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