Rabbit Recombinant Monoclonal SGLT2 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435222.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435224.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435221.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (AB320087)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | WB | mIHC | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Target data
Function
Electrogenic Na(+)-coupled sugar symporter that actively transports D-glucose at the plasma membrane, with a Na(+) to sugar coupling ratio of 1:1 (PubMed:20980548, PubMed:28592437, PubMed:34880493, PubMed:37217492, PubMed:38057552). Transporter activity is driven by a transmembrane Na(+) electrochemical gradient set by the Na(+)/K(+) pump (PubMed:20980548, PubMed:28592437, PubMed:34880493). Unlike SLC5A1/SGLT1, requires the auxiliary protein PDZK1IP1/MAP17 for full transporter activity (PubMed:37217492). Has a primary role in D-glucose reabsorption from glomerular filtrate across the brush border of the early proximal tubules of the kidney (By similarity).
Alternative names
SGLT2, SLC5A2, Sodium/glucose cotransporter 2, Na(+)/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2
Recommended products
Rabbit Recombinant Monoclonal SGLT2 antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29167-46
- Purification technique
- Affinity purification Protein A
- Specificity
The immunogen shares no sequence similarity with SLC5A1. Based on the immunogen sequence, we do not expect this antibody to cross-react with SLC5A1. No cross-reactivity testing has been performed.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab320087 is the carrier-free version of Anti-SGLT2 antibody [EPR29167-46] ab320086.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SGLT2 also known as sodium-glucose co-transporter 2 is a protein responsible for reabsorbing glucose in the renal proximal convoluted tubule. It carries glucose along with sodium ions across cell membranes enabling glucose reabsorption from the glomerular filtrate back into the bloodstream a process essential for the maintenance of glucose homeostasis. This protein has a molecular weight of approximately 75 kDa. SGLT2 is mostly expressed in the renal epithelial cells located within the kidney's proximal tubule.
- Biological function summary
Sodium-glucose co-transporter 2 plays a central role in renal glucose reabsorption. It operates as part of a vital mechanism in the kidney where it engages in the active transport of glucose against its concentration gradient facilitated by the sodium gradient produced by the Na⁺/K⁺-ATPase pump. This transporter ensures minimal loss of glucose in urine maintaining energy balance and blood glucose levels.
- Pathways
SGLT2 is involved in glucose and sodium transport pathways critical for glycolytic and gluconeogenic processes. These pathways are key for energy production and glucose homeostasis. It works closely with proteins like GLUT2 which operates in the basolateral membrane of renal tubular cells to further transfer reabsorbed glucose into the bloodstream. Together these proteins form a tightly regulated network that maintains normal blood glucose levels.
- Associated diseases and disorders
SGLT2 has a significant impact on diabetes and cardiovascular disease. Inhibitors of SGLT2 are effective treatments for type 2 diabetes by preventing glucose reabsorption leading to increased glucose excretion through urine and improved glycemic control. The glucose-lowering effect also contributes to reducing risks associated with cardiovascular diseases. In this context SGLT2 interacts with protein targets like insulin where its modulation offers therapeutic efficacy in managing hyperglycemia.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435222.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)
This data was developed using Anti-SGLT2 antibody [EPR29167-46] ab320086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SGLT2 with Anti-SGLT2 antibody [EPR29167-46] ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on human cerebrum.
The section was incubated with Anti-SGLT2 antibody [EPR29167-46] ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435224.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)
This data was developed using Anti-SGLT2 antibody [EPR29167-46] ab320086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a SLC5A2 expression vector containing a his tag and (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling SGLT2 with Anti-SGLT2 antibody [EPR29167-46] ab320086 at 1/2000 (0.254 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T transfected with a SLC5A2 expression vector containing a his tag. No staining on (B) HEK-293T transfected with empty vector containing a his tag.
The section was incubated with Anti-SGLT2 antibody [EPR29167-46] ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)
This data was developed using Anti-SGLT2 antibody [EPR29167-46] ab320086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling SGLT2 with Anti-SGLT2 antibody [EPR29167-46] ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: No staining on human pancreas.
The section was incubated with Anti-SGLT2 antibody [EPR29167-46] ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087), expandable thumbnail](https://content.abcam.com/products/images/sglt2-antibody-epr29167-46-bsa-and-azide-free-ab320087--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img435221.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SGLT2 antibody [EPR29167-46] - BSA and Azide free (ab320087)
This data was developed using Anti-SGLT2 antibody [EPR29167-46] ab320086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SGLT2 with Anti-SGLT2 antibody [EPR29167-46] ab320086 at 1/1000 (0.508 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney (PMID: 32488949).
The section was incubated with Anti-SGLT2 antibody [EPR29167-46] ab320086 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com